scholarly journals A “weak acid and weak base” type fluorescent probe for sensing pH: mechanism and application in living cells

RSC Advances ◽  
2019 ◽  
Vol 9 (36) ◽  
pp. 20982-20988 ◽  
Author(s):  
Chunpeng Jiao ◽  
Jingxiang Pang ◽  
Li Shen ◽  
Wenjuan Lu ◽  
Pingping Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Living Cells ◽  
Weak Acid ◽  
Weak Base

When the probe was in the protonation process, the fluorescence intensity gradually decreased, whereas when the probe was in the deprotonation process, the fluorescence intensity gradually increased.

scholarly journals A Rare Natural Benzo[k,l]xanthene as a Turn-Off Fluorescent Sensor for Cu2+ Ion

2020 ◽  
Vol 21 (18) ◽  
pp. 6933
Author(s):  
Giuseppe Floresta ◽  
Nunzio Cardullo ◽  
Carmela Spatafora ◽  
Antonio Rescifina ◽  
Corrado Tringali
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Copper Ion ◽  
Living Cells ◽  
A Value ◽  
Wide Range ◽  
Catechol Group

Rapid and efficient analyses of copper ions are crucial to providing key information for Cu2+ in living cells because of their biological importance. In this study, we reported one new turn-off fluorescent sensor for Cu2+ with a benzo[k,l]xanthene core, which served as an efficient cation sensor for copper ion over a wide range of other cations (Na+, K+, Ag+, Hg2+, Cd2+, Co2+, Ni2+, Zn2+, Mg2+, and Fe3+) owing to the catechol group in the aromatic core. The sensor showed selectivity for Cu2+ over other ions; the logKβ for Cu2+ binding to compound 1 had a value of 13.265. In the presence of Cu2+, sensor 1 provided significant fluorescence decrement; Co2+, and Ni2+ caused a fluorescence decrement when employed at a higher concentration than Cu2+, while Na+, K+, Hg2+, Cd2+, Zn2+, and Mg2+ metal ions produced only minor changes in fluorescence intensity. Fluorescence experiments demonstrate that compound 1 may have an application as a fluorescent probe for detecting Cu2+ with a limit of detection of 0.574 µM.

scholarly journals Rational design of a selective and sensitive “turn-on” fluorescent probe for monitoring and imaging hydrogen peroxide in living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (56) ◽  
pp. 35093-35098
Author(s):  
Jing Lu ◽  
Liang Ji ◽  
Yanyan Yu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Rational Design ◽  
Living Cells ◽  
Turn On

A fluorescent probe N-Py-BO was well designed and synthesized and its ability for detecting H2O2 by fluorescence intensity was evaluated.

Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  
Keyword(s):  
Microwave Heating ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Heating Process ◽  
X Ray ◽  
Doped Carbon Dots ◽  
Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

scholarly journals Structure modulation on fluorescent probes for biothiols and the reversible imaging of glutathione in living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 21116-21126
Author(s):  
Yu Li ◽  
Li Chen ◽  
Yan Zhu ◽  
Liming Chen ◽  
Xianglin Yu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Structure Modulation

A reversible fluorescent probe for GSH was obtained through structure modulation, by which the intracellular GSH fluctuation was imaged.

A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

2021 ◽  
Author(s):  
Bochao Chen ◽  
Shumei Mao ◽  
Yanyan Sun ◽  
Liyuan Sun ◽  
Ning Ding ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Diabetic Mouse ◽  
Living Cells ◽  
Pancreatic Tissue ◽  
Diabetic Mice ◽  
Mice Model ◽  
Viscosity Changes

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which was applied to detect the intracellular viscosity and imagine pancreatic tissue in diabetic mouse model.

A smart mitochondria-targeting TP-NIR fluorescent probe for selective and sensitive sensing H2S in living cells and mice

2021 ◽  
Author(s):  
Qiaomei Yang ◽  
Liyi Zhou ◽  
Longpeng Peng ◽  
Gangqiang Yuan ◽  
Haiyuan Ding ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Signal Molecules ◽  
Two Photon ◽  
Mitochondria Targeting

Hydrogen sulfide (H2S) is one of the important gaseous signal molecules and plays key roles in various biologically crucial processes. In this work, we report a novel two-photon near-infrared (TP-NIR)...

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