scholarly journals A label-free immunosensor based on PHEMA/graphene oxide nanocomposite for simultaneous electrochemical determination of alpha fetoprotein

RSC Advances ◽  
2019 ◽  
Vol 9 (30) ◽  
pp. 17187-17193 ◽  
Author(s):  
Ying Liang ◽  
Xiaoqing Zhao ◽  
Na Wang ◽  
Jing Wang ◽  
Hou Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Determination ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Ultrasensitive Detection ◽  
Hydroxyethyl Methacrylate ◽  
Label Free

An electrochemical immunosensor based on poly(2-hydroxyethyl methacrylate) (PHEMA)/graphene oxide (GO) nanocomposite was designed in a simple way for the ultrasensitive detection of tumor makers (alpha-fetoprotein, AFP as a model).

Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

scholarly journals A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 24 ◽  
Author(s):  
Shanshan Wei ◽  
Haolin Xiao ◽  
Liangli Cao ◽  
Zhencheng Chen
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Prussian Blue ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Determination ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

Polydopamine and graphene oxide synergistically modified Prussian blue electrochemical immunosensor for the detection of alpha-fetoprotein with enhanced stability and sensibility

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38176-38182 ◽  
Author(s):  
Ren-zhong Zhang ◽  
Bo-guang Pan ◽  
Hai-ning Wang ◽  
Jian-ming Dan ◽  
Cheng-lin Hong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Prussian Blue ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Amperometric Immunosensor ◽  
Highly Sensitive ◽  
Enhanced Stability

In this paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein based on multi-functional gold nanoparticles–polydopamine-Prussian blue–graphene oxide nanocomposites (Au–PDA-PB–GO) was fabricated.

scholarly journals Correction to: A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (6) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Beta Amyloid ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene

Construction of electrochemical immunosensor based on redox hydrogel for ultrasensitive detection of carcinoembryonic antigen

2021 ◽  
Author(s):  
Xiangrong Huang ◽  
Na Wu ◽  
Wenxiu Liu ◽  
Yazhuo Shang ◽  
Honglai Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Detection ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free

In this work, a novel redox hydrogel was proposed for ultrasensitive label-free electrochemical detection of carcinoembryonic antigen (CEA). The redox hydrogel composed by cellulose nanocrystalline (CNC), methylene blue (MB), multi-walled...

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