scholarly journals A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 24 ◽  
Author(s):  
Shanshan Wei ◽  
Haolin Xiao ◽  
Liangli Cao ◽  
Zhencheng Chen
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Prussian Blue ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Determination ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

scholarly journals A highly sensitive biosensor based on methacrylated graphene oxide-grafted polyaniline for ascorbic acid determination

2020 ◽  
Vol 9 (1) ◽  
pp. 760-767 ◽  
Author(s):  
Seyed Morteza Naghib ◽  
Farahnaz Behzad ◽  
Mehdi Rahmanian ◽  
Yasser Zare ◽  
Kyong Yop Rhee
Keyword(s):  
Ascorbic Acid ◽  
Graphene Oxide ◽  
Current Response ◽  
Functionalized Graphene ◽  
Serum Samples ◽  
Promising Candidate ◽  
Graphene Nanocomposites ◽  
Selective Determination ◽  
Highly Sensitive

AbstractFunctionalized graphene-based nanocomposites have opened new windows to address some challenges for increasing the sensitivity, accuracy and functionality of biosensors. Polyaniline (PANI) is one of the most potentially promising and technologically important conducting polymers, which brings together the electrical features of metals with intriguing properties of plastics including facile processing and controllable chemical and physical properties. PANI/graphene nanocomposites have attracted intense interest in various fields due to unique physicochemical properties including high conductivity, facile preparation and intriguing redox behavior. In this article, a functionalized graphene-grafted nanostructured PANI nanocomposite was applied for determining the ascorbic acid (AA) level. A significant current response was observed after treating the electrode surface with methacrylated graphene oxide (MeGO)/PANI nanocomposite. The amperometric responses showed a robust linear range of 8–5,000 µM and detection limit of 2 µM (N = 5). Excellent sensor selectivity was demonstrated in the presence of electroactive components interfering species, commonly found in real serum samples. This sensor is a promising candidate for rapid and selective determination of AA.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

3D graphene oxide-cobalt oxide polyhedrons for highly sensitive non-enzymatic electrochemical determination of hydrogen peroxide

2017 ◽  
Vol 253 ◽  
pp. 773-783 ◽  
Author(s):  
Sakthivel Kogularasu ◽  
Mani Govindasamy ◽  
Shen-Ming Chen ◽  
Muthumariappan Akilarasan ◽  
Veerappan Mani
Keyword(s):  
Hydrogen Peroxide ◽  
Graphene Oxide ◽  
Cobalt Oxide ◽  
Electrochemical Determination ◽  
3D Graphene ◽  
Highly Sensitive

Highly sensitive simultaneous electrochemical determination of hydroquinone, catechol and resorcinol based on carbon dot/reduced graphene oxide composite modified electrodes

2015 ◽  
Vol 7 (15) ◽  
pp. 6089-6094 ◽  
Author(s):  
Wuxiang Zhang ◽  
Jianzhong Zheng ◽  
Zhongqiu Lin ◽  
Ling Zhong ◽  
Jiangu Shi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Modified Electrodes ◽  
Electrochemical Determination ◽  
Schematic Diagram ◽  
Carbon Dot ◽  
Oxide Composite ◽  
Reduced Graphene ◽  
Highly Sensitive

A schematic diagram of the preparation processes of CD/r-GO and the CD/r-GO electrode used for the detection of dihydroxybenzene isomers.

scholarly journals A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of PSA as a Specific Diagnostic Marker of Prostate Cancer

2020 ◽  
Vol 23 ◽  
pp. 243-258 ◽  
Author(s):  
Shokoufeh Hassani ◽  
Armin Salek Maghsoudi ◽  
Milad Rezaei Akmal ◽  
Soheila Rahmani Rahmani ◽  
Pouria Sarihi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Differential Pulse ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive ◽  
Set Up

Purpose: The current project aimed to design a simple, highly sensitive, and economical label-free electrochemical aptasensor for determination of prostate-specific antigen (PSA), as the gold standard biomarker for prostate cancer diagnosis. The aptasensor was set up using a screen-printed carbon electrode (SPCE) modified by gold nanoparticles (Au NPs) conjugated to thiolated aptamers. Methods: Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were implemented for electrochemical (EC) characterization of the aptasensor. The determination of PSA was also performed through differential pulse voltammetry (DPV) in [Fe (CN) 6]3-/4- electrolyte solution. Results: The present aptasensor was shown an outstanding linear response in the concentration range of 1 pg/mL - 200 ng/mL with a remarkably lower limit of detection of 0.077 pg/mL. The optimum concentration for PSA separation and the optimum incubation time for antigen-aptamer binding were determined by observing and electing the highest electrochemical responses in a specified time or concentration. Conclusion: According to the results of the specificity tests, the designed aptasensor did not show any significant interactions with other analytes in real samples. Clinical functionality of the aptasensor was appraised in serum samples of healthy individuals and patients examining the PSA level through the fabricated aptasensor and the reference methods. Both methods are comparable in sensitivity. The present fabricated PSA aptasensor with substantial characteristics of ultra-sensitivity and cost-effectiveness can be conventionally built and used for the routine check-up of the men for prostate problems.

Polydopamine and graphene oxide synergistically modified Prussian blue electrochemical immunosensor for the detection of alpha-fetoprotein with enhanced stability and sensibility

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38176-38182 ◽  
Author(s):  
Ren-zhong Zhang ◽  
Bo-guang Pan ◽  
Hai-ning Wang ◽  
Jian-ming Dan ◽  
Cheng-lin Hong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Prussian Blue ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Amperometric Immunosensor ◽  
Highly Sensitive ◽  
Enhanced Stability

In this paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein based on multi-functional gold nanoparticles–polydopamine-Prussian blue–graphene oxide nanocomposites (Au–PDA-PB–GO) was fabricated.

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