Polydopamine and graphene oxide synergistically modified Prussian blue electrochemical immunosensor for the detection of alpha-fetoprotein with enhanced stability and sensibility

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38176-38182 ◽  
Author(s):  
Ren-zhong Zhang ◽  
Bo-guang Pan ◽  
Hai-ning Wang ◽  
Jian-ming Dan ◽  
Cheng-lin Hong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Prussian Blue ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Amperometric Immunosensor ◽  
Highly Sensitive ◽  
Enhanced Stability

In this paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein based on multi-functional gold nanoparticles–polydopamine-Prussian blue–graphene oxide nanocomposites (Au–PDA-PB–GO) was fabricated.

scholarly journals A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 24 ◽  
Author(s):  
Shanshan Wei ◽  
Haolin Xiao ◽  
Liangli Cao ◽  
Zhencheng Chen
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Prussian Blue ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Determination ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

scholarly journals A label-free immunosensor based on PHEMA/graphene oxide nanocomposite for simultaneous electrochemical determination of alpha fetoprotein

RSC Advances ◽  
2019 ◽  
Vol 9 (30) ◽  
pp. 17187-17193 ◽  
Author(s):  
Ying Liang ◽  
Xiaoqing Zhao ◽  
Na Wang ◽  
Jing Wang ◽  
Hou Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Determination ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Ultrasensitive Detection ◽  
Hydroxyethyl Methacrylate ◽  
Label Free

An electrochemical immunosensor based on poly(2-hydroxyethyl methacrylate) (PHEMA)/graphene oxide (GO) nanocomposite was designed in a simple way for the ultrasensitive detection of tumor makers (alpha-fetoprotein, AFP as a model).

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