scholarly journals Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement

2020 ◽  
Vol 2 (5) ◽  
pp. 1894-1903
Author(s):  
Abdullah Al Masud ◽  
W. Elliott Martin ◽  
Faruk H. Moonschi ◽  
So Min Park ◽  
Bernadeta R. Srijanto ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Zero Mode ◽  
Single Molecule Fluorescence ◽  
Mixed Metal

Mixed metal ZMWs tune single molecule fluorescence enhancement.

scholarly journals Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)

2013 ◽  
Vol 21 (1) ◽  
pp. 1189 ◽  
Author(s):  
Mary Williard Elting ◽  
Sabrina R. Leslie ◽  
L. Stirling Churchman ◽  
Jonas Korlach ◽  
Christopher M. J. McFaul ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Zero Mode ◽  
Background Suppression ◽  
Single Molecule Fluorescence ◽  
Convex Lens

Single-molecule fluorescence imaging of kinesin using linear zero-mode waveguides

2016 ◽  
Author(s):  
Yuki Morita ◽  
Kazuya Fujimoto ◽  
Ryota Iino ◽  
Michio Tomishige ◽  
Hirofumi Shintaku ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Zero Mode ◽  
Single Molecule Fluorescence

scholarly journals Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet

2020 ◽  
Vol 2 (9) ◽  
pp. 4153-4160 ◽  
Author(s):  
Mikhail Baibakov ◽  
Aleksandr Barulin ◽  
Prithu Roy ◽  
Jean-Benoît Claude ◽  
Satyajit Patra ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Zero Mode ◽  
Metallic Films ◽  
Deep Ultraviolet ◽  
Single Molecule Analysis

Nanoapertures milled in metallic films called zero-mode waveguides (ZMWs) overcome the limitations of classical confocal microscopes by enabling single molecule analysis at micromolar concentrations with improved fluorescence brightness.

scholarly journals Nanoaperture fabrication via colloidal lithography for single molecule fluorescence imaging

2019 ◽  
Author(s):  
Ryan M. Jamiolkowski ◽  
Kevin Y Chen ◽  
Shane A. Fiorenza ◽  
Alyssa M. Tate ◽  
Shawn H. Pfeil ◽  
...  
Keyword(s):  
Single Molecule ◽  
Self Assembly ◽  
Zero Mode ◽  
Thin Metal Film ◽  
Polystyrene Microspheres ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies ◽  
Observation Volume ◽  
Sophisticated Equipment ◽  
Sub Wavelength

AbstractIn single molecule fluorescence studies, background emission from labeled substrates often restricts their concentrations to non-physiological nanomolar values. One approach to address this challenge is the use of zero-mode waveguides (ZMWs), nanoscale holes in a thin metal film that physically and optically confine the observation volume allowing much higher concentrations of fluorescent substrates. Standard fabrication of ZMWs utilizes slow and costly E-beam nano-lithography. Herein, ZMWs are made using a self-assembled mask of polystyrene microspheres, enabling fabrication of thousands of ZMWs in parallel without sophisticated equipment. Polystyrene 1 μm dia. microbeads self-assemble on a glass slide into a hexagonal array, forming a mask for the deposition of metallic posts in the inter-bead interstices. The width of those interstices (and subsequent posts) is adjusted within 100-300 nm by partially fusing the beads at the polystyrene glass transition temperature. The beads are dissolved in toluene, aluminum or gold cladding is deposited around the posts, and those are dissolved, leaving behind an array ZMWs. Parameter optimization and the performance of the ZMWs are presented. By using colloidal self-assembly, typical laboratories can make use of sub-wavelength ZMW technology avoiding the availability and expense of sophisticated clean-room environments and equipment.

Combining gold nanoparticle antennas with single-molecule fluorescence resonance energy transfer (smFRET) to study DNA hairpin dynamics

Nanoscale ◽  
2018 ◽  
Vol 10 (14) ◽  
pp. 6611-6619 ◽  
Author(s):  
Jinyong Hu ◽  
Meiyan Wu ◽  
Li Jiang ◽  
Zhensheng Zhong ◽  
Zhangkai Zhou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Gold Nanoparticle ◽  
Fluorescence Resonance Energy Transfer ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Fluorescence Resonance

Gold nanoparticle antennas as a promising platform not only for fluorescence enhancement but also for the studies of single-molecule kinetics.

scholarly journals Quantum Yield Limits for the Detection of Single-Molecule Fluorescence Enhancement by a Gold Nanorod

ACS Photonics ◽  
2020 ◽  
Vol 7 (9) ◽  
pp. 2498-2505
Author(s):  
Xuxing Lu ◽  
Gang Ye ◽  
Deep Punj ◽  
Ryan C. Chiechi ◽  
Michel Orrit
Keyword(s):  
Quantum Yield ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Gold Nanorod ◽  
Single Molecule Fluorescence

Real-Time Imaging of Single-Molecule Fluorescence with a Zero-Mode Waveguide for the Analysis of Protein−Protein Interaction

2008 ◽  
Vol 80 (15) ◽  
pp. 6018-6022 ◽  
Author(s):  
Takeo Miyake ◽  
Takashi Tanii ◽  
Hironori Sonobe ◽  
Rena Akahori ◽  
Naonobu Shimamoto ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Protein Interaction ◽  
Zero Mode ◽  
Single Molecule Fluorescence ◽  
Real Time Imaging ◽  
Protein Protein Interaction

Single-molecule fluorescence enhancement of a near-infrared dye by gold nanorods using DNA transient binding

2018 ◽  
Vol 20 (31) ◽  
pp. 20468-20475 ◽  
Author(s):  
Weichun Zhang ◽  
Martín Caldarola ◽  
Xuxing Lu ◽  
Biswajit Pradhan ◽  
Michel Orrit
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Plasmonic Nanostructures ◽  
New Materials ◽  
Single Molecule Fluorescence ◽  
Molecular Fluorescence ◽  
Near Infrared Dye

Fluorescence enhancement by plasmonic nanostructures enables the optical detection of single molecules with weak fluorescence, extending the scope of molecular fluorescence imaging to new materials and systems.

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