Real-Time Imaging of Single-Molecule Fluorescence with a Zero-Mode Waveguide for the Analysis of Protein−Protein Interaction

2008 ◽  
Vol 80 (15) ◽  
pp. 6018-6022 ◽  
Author(s):  
Takeo Miyake ◽  
Takashi Tanii ◽  
Hironori Sonobe ◽  
Rena Akahori ◽  
Naonobu Shimamoto ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Protein Interaction ◽  
Zero Mode ◽  
Single Molecule Fluorescence ◽  
Real Time Imaging ◽  
Protein Protein Interaction

scholarly journals Mechanism of Coordination of the Bacteriophage T4 DNA Packaging Motor Analyzed by Real-Time Single Molecule Fluorescence Assay

2016 ◽  
Vol 110 (3) ◽  
pp. 46a ◽  
Author(s):  
Li Dai ◽  
Digvijay Singh ◽  
Reza Vafabakhsh ◽  
Marthandan Mahalingam ◽  
Vishal Kottadiel ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Fluorescence Assay ◽  
Single Molecule Fluorescence ◽  
Dna Packaging Motor

scholarly journals Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2826 ◽  
Author(s):  
Shangguo Hou ◽  
Courtney Johnson ◽  
Kevin Welsher
Keyword(s):  
Fluorescence Spectroscopy ◽  
Real Time ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Active Feedback

Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements. However, the act of tethering a molecule requires that the molecule be removed from its environment. This is especially perturbative when measuring biomolecules such as enzymes, which may rely on the non-equilibrium and crowded cellular environment for normal function. A method which may be able to un-tether single molecule fluorescence spectroscopy is real-time 3D single particle tracking (RT-3D-SPT). RT-3D-SPT uses active feedback to effectively lock-on to freely diffusing particles so they can be measured continuously with up to photon-limited temporal resolution over large axial ranges. This review gives an overview of the various active feedback 3D single particle tracking methods, highlighting specialized detection and excitation schemes which enable high-speed real-time tracking. Furthermore, the combination of these active feedback methods with simultaneous live-cell imaging is discussed. Finally, the successes in real-time 3D single molecule tracking (RT-3D-SMT) thus far and the roadmap going forward for this promising family of techniques are discussed.

scholarly journals Analyzing DNA Packaging Initiation of Bacteriophage T4 by a Real-Time Single Molecule Fluorescence Assay

2012 ◽  
Vol 102 (3) ◽  
pp. 643a
Author(s):  
Reza Vafabakhsh ◽  
Kiran Kondabagil ◽  
Li Dai ◽  
Zhihong Zhang ◽  
Venigalla B. Rao ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Fluorescence Assay ◽  
Single Molecule Fluorescence

scholarly journals Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)

2013 ◽  
Vol 21 (1) ◽  
pp. 1189 ◽  
Author(s):  
Mary Williard Elting ◽  
Sabrina R. Leslie ◽  
L. Stirling Churchman ◽  
Jonas Korlach ◽  
Christopher M. J. McFaul ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Zero Mode ◽  
Background Suppression ◽  
Single Molecule Fluorescence ◽  
Convex Lens

Single-molecule fluorescence imaging of kinesin using linear zero-mode waveguides

2016 ◽  
Author(s):  
Yuki Morita ◽  
Kazuya Fujimoto ◽  
Ryota Iino ◽  
Michio Tomishige ◽  
Hirofumi Shintaku ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Zero Mode ◽  
Single Molecule Fluorescence

scholarly journals 2P-126 Real time imaging of tRNA dynamics with a zero-mode waveguides during translation(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S94-S95
Author(s):  
Sotaro Uemura ◽  
Yoshihiro Shimizu ◽  
Ryo Iizuka ◽  
Takuya Ueda ◽  
Rena Akahori ◽  
...  
Keyword(s):  
Real Time ◽  
Annual Meeting ◽  
Zero Mode ◽  
Real Time Imaging ◽  
Biophysical Society

Solid-state nanopore analysis on conformation change of p53TAD–MDM2 fusion protein induced by protein–protein interaction

Nanoscale ◽  
2018 ◽  
Vol 10 (36) ◽  
pp. 17227-17235 ◽  
Author(s):  
Hongsik Chae ◽  
Dong-Kyu Kwak ◽  
Mi-Kyung Lee ◽  
Seung-Wook Chi ◽  
Ki-Bum Kim
Keyword(s):  
Solid State ◽  
Fusion Protein ◽  
Single Molecule ◽  
Protein Interaction ◽  
Conformation Change ◽  
Protein Protein Interaction ◽  
Translocation Event

Nutlin-3-induced conformation change of p53TAD–MDM2 fusion protein were probed by single molecule translocation event using solid-state nanopores.

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