scholarly journals Deformability-induced lift force in spiral microchannels for cell separation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 614-625 ◽  
Author(s):  
Ewa Guzniczak ◽  
Oliver Otto ◽  
Graeme Whyte ◽  
Nicholas Willoughby ◽  
Melanie Jimenez ◽  
...  
Keyword(s):  
Cell Separation ◽  
Mammalian Cells ◽  
Lift Force ◽  
Label Free ◽  
Dean Flow ◽  
Spiral Microchannel

We introduce a novel combination of deformability-induced lift force (FD) and secondary Dean flow in spiral microchannel translated into a label-free purification approach applicable to mammalian cells, processing of millions of cells per min, up to high purities and recovery.

scholarly journals Double spiral microchannel for label-free tumor cell separation and enrichment

Lab on a Chip ◽  
2012 ◽  
Vol 12 (20) ◽  
pp. 3952 ◽  
Author(s):  
Jiashu Sun ◽  
Mengmeng Li ◽  
Chao Liu ◽  
Yi Zhang ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Separation ◽  
Label Free ◽  
Double Spiral ◽  
Spiral Microchannel

scholarly journals Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

2019 ◽  
Vol 2 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Colin Chung ◽  
Martin Waterfall ◽  
Steve Pells ◽  
Anoop Menachery ◽  
Stewart Smith ◽  
...  
Keyword(s):  
Dielectric Properties ◽  
Cell Separation ◽  
Mammalian Cells ◽  
Uniform Electric Field ◽  
Cell Diameter ◽  
Label Free ◽  
Myeloma Cells ◽  
A Value ◽  
A Cell

Abstract Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many studies have focused on the DEP cross-over frequency fxo1, where cells in a non-uniform electric field undergo a transition from negative to positive DEP. Determination of fxo1 provides a value for the membrane capacitance from the cell diameter, the means to monitor changes in cell morphology and viability, and the information required when devising DEP cell separation protocols. In this paper we describe the first systematic measurements of the second DEP cross-over frequency fxo2 that occurs at much higher frequencies. Theory indicates that fxo2 is sensitive to the internal dielectric properties of a cell, and our experiments on murine myeloma cells reveal that these properties exhibit temporal changes that are sensitive to both the osmolality and temperature of the cell suspending medium.

scholarly journals xCELLanalyzer: A Framework for the Analysis of Cellular Impedance Measurements for Mode of Action Discovery

2019 ◽  
Vol 24 (3) ◽  
pp. 213-223 ◽  
Author(s):  
Raimo Franke ◽  
Bettina Hinkelmann ◽  
Verena Fetz ◽  
Theresia Stradal ◽  
Florenz Sasse ◽  
...  
Keyword(s):  
Data Analysis ◽  
Bioactive Compounds ◽  
Mode Of Action ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Label Free ◽  
Synthesis Inhibitor ◽  
Analysis Pipeline ◽  
Bioactive Natural Products ◽  
Data Analysis Pipeline

Mode of action (MoA) identification of bioactive compounds is very often a challenging and time-consuming task. We used a label-free kinetic profiling method based on an impedance readout to monitor the time-dependent cellular response profiles for the interaction of bioactive natural products and other small molecules with mammalian cells. Such approaches have been rarely used so far due to the lack of data mining tools to properly capture the characteristics of the impedance curves. We developed a data analysis pipeline for the xCELLigence Real-Time Cell Analysis detection platform to process the data, assess and score their reproducibility, and provide rank-based MoA predictions for a reference set of 60 bioactive compounds. The method can reveal additional, previously unknown targets, as exemplified by the identification of tubulin-destabilizing activities of the RNA synthesis inhibitor actinomycin D and the effects on DNA replication of vioprolide A. The data analysis pipeline is based on the statistical programming language R and is available to the scientific community through a GitHub repository.

Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50–1000 Mammalian Cells

2021 ◽  
Author(s):  
Kendall Martin ◽  
Tong Zhang ◽  
Tai-Tu Lin ◽  
Amber N. Habowski ◽  
Rui Zhao ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Label Free ◽  
One Pot ◽  
Proteome Profiling

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux

PLoS Biology ◽  
2019 ◽  
Vol 17 (12) ◽  
pp. e3000553 ◽  
Author(s):  
Patrick A. Sandoz ◽  
Christopher Tremblay ◽  
F. Gisou van der Goot ◽  
Mathieu Frechin
Keyword(s):  
Refractive Index ◽  
Mass Flux ◽  
Mammalian Cells ◽  
Dry Mass ◽  
Label Free ◽  
Index Maps

scholarly journals Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3445-3460
Author(s):  
Kerwin Kwek Zeming ◽  
Yuko Sato ◽  
Lu Yin ◽  
Nai-Jia Huang ◽  
Lan Hiong Wong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Closed Loop ◽  
Lateral Displacement ◽  
Label Free ◽  
Hematopoietic Stem ◽  
Deterministic Lateral Displacement ◽  
Dean Flow ◽  
Flow Fractionation ◽  
Human Rbcs

Developments in Dean flow fractionation (DFF) and deterministic lateral displacement (DLD) for label-free purification of cultured RBCs from human hematopoietic stem cells. An advancement in sorting and closed-loop manufacturing of viable human RBCs.

3D-printed smartphone-based device for label-free cell separation

2017 ◽  
Vol 1 (3) ◽  
pp. 155-164 ◽  
Author(s):  
Reza Amin ◽  
Stephanie Knowlton ◽  
Joshua Dupont ◽  
Johann S Bergholz ◽  
Ashwini Joshi ◽  
...  
Keyword(s):  
Cell Separation ◽  
Free Cell ◽  
Label Free ◽  
3D Printed
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