scholarly journals Double spiral microchannel for label-free tumor cell separation and enrichment

Lab on a Chip ◽  
2012 ◽  
Vol 12 (20) ◽  
pp. 3952 ◽  
Author(s):  
Jiashu Sun ◽  
Mengmeng Li ◽  
Chao Liu ◽  
Yi Zhang ◽  
Dingbin Liu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Separation ◽  
Label Free ◽  
Double Spiral ◽  
Spiral Microchannel

scholarly journals Enhanced Separation Efficiency and Purity of Circulating Tumor Cells Based on the Combined Effects of Double Sheath Fluids and Inertial Focusing

2021 ◽  
Vol 9 ◽  
Author(s):  
Bo-Wen Li ◽  
Kun Wei ◽  
Qi-Qi Liu ◽  
Xian-Ge Sun ◽  
Ning Su ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Purity ◽  
Separation Efficiency ◽  
Control Group ◽  
Combined Effects ◽  
Label Free ◽  
Inertial Focusing ◽  
Double Spiral ◽  
Spiral Microchannel

Circulating tumor cells (CTCs) play a crucial role in solid tumor metastasis, but obtaining high purity and viability CTCs is a challenging task due to their rarity. Although various works using spiral microchannels to isolate CTCs have been reported, the sorting purity of CTCs has not been significantly improved. Herein, we developed a novel double spiral microchannel for efficient separation and enrichment of intact and high-purity CTCs based on the combined effects of two-stage inertial focusing and particle deflection. Particle deflection relies on the second sheath to produce a deflection of the focused sample flow segment at the end of the first-stage microchannel, allowing larger particles to remain focused and entered the second-stage microchannel while smaller particles moved into the first waste channel. The deflection of the focused sample flow segment was visualized. Testing by a binary mixture of 10.4 and 16.5 μm fluorescent microspheres, it showed 16.5 μm with separation efficiency of 98% and purity of 90% under the second sheath flow rate of 700 μl min−1. In biological experiments, the average purity of spiked CTCs was 74% at a high throughput of 1.5 × 108 cells min−1, and the recovery was more than 91%. Compared to the control group, the viability of separated cells was 99%. Finally, we validated the performance of the double spiral microchannel using clinical cancer blood samples. CTCs with a concentration of 2–28 counts ml−1 were separated from all 12 patients’ peripheral blood. Thus, our device could be a robust and label-free liquid biopsy platform in inertial microfluidics for successful application in clinical trials.

Inertial Microfluidics for Separation and Detection of Tumor Cells

2013 ◽  
Author(s):  
Jiashu Sun
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Separation ◽  
Microfluidic Channel ◽  
Isothermal Amplification ◽  
Rare Tumor ◽  
Label Free ◽  
Ck19 Mrna ◽  
Hydrodynamic Effects ◽  
Mcf 7

We report on the development of a curved microfluidic channel that allows rapid and continuous size-based rare tumor cell separation from blood in a label-free manner by exploiting the hydrodynamic effects. The separated tumor cells are trapped and enriched on an integrated polycarbonate filter glued on top of the outlet reservoir of microchannels. CK19 mRNA of MCF-7 cells are detected by loop-mediated isothermal amplification (LAMP).

scholarly journals Deformability-induced lift force in spiral microchannels for cell separation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 614-625 ◽  
Author(s):  
Ewa Guzniczak ◽  
Oliver Otto ◽  
Graeme Whyte ◽  
Nicholas Willoughby ◽  
Melanie Jimenez ◽  
...  
Keyword(s):  
Cell Separation ◽  
Mammalian Cells ◽  
Lift Force ◽  
Label Free ◽  
Dean Flow ◽  
Spiral Microchannel

We introduce a novel combination of deformability-induced lift force (FD) and secondary Dean flow in spiral microchannel translated into a label-free purification approach applicable to mammalian cells, processing of millions of cells per min, up to high purities and recovery.

scholarly journals The efficiency of tumor cell purging using immunomagnetic CD34+ cell separation systems

1997 ◽  
Vol 19 (12) ◽  
pp. 1239-1246 ◽  
Author(s):  
A Roots-Weiß ◽  
C Papadimitriou ◽  
H Serve ◽  
B Hoppe ◽  
M Koenigsmann ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Separation ◽  
Separation Systems

ClearCell FX: A microfluidic system for label-free circulating tumor cell enrichment.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e22023-e22023 ◽  
Author(s):  
Andrew Wu ◽  
Ali Asgar Bhagat ◽  
Man Chun Leong ◽  
Chwee Teck Lim
Keyword(s):  
Tumor Cell ◽  
Circulating Tumor Cell ◽  
Microfluidic System ◽  
Label Free ◽  
Cell Enrichment

3D-printed smartphone-based device for label-free cell separation

2017 ◽  
Vol 1 (3) ◽  
pp. 155-164 ◽  
Author(s):  
Reza Amin ◽  
Stephanie Knowlton ◽  
Joshua Dupont ◽  
Johann S Bergholz ◽  
Ashwini Joshi ◽  
...  
Keyword(s):  
Cell Separation ◽  
Free Cell ◽  
Label Free ◽  
3D Printed
Sign in / Sign up

Export Citation Format

Share Document