Lactobacillus rhamnosus from human breast milk shows therapeutic function against foodborne infection by multi-drug resistant Escherichia coli in mice

2020 ◽  
Vol 11 (1) ◽  
pp. 435-447 ◽  
Author(s):  
Na Li ◽  
Bing Pang ◽  
Guanwen Liu ◽  
Xixi Zhao ◽  
Xiaoguang Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Breast Milk ◽  
Therapeutic Efficacy ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Drug Resistant ◽  
Human Breast ◽  
E Coli ◽  
Therapeutic Function ◽  
Foodborne Infection

Lactobacillus rhamnosus shows higher therapeutic efficacy than antibiotic to treat drug-resistant E. coli infection in aspects of fast reducing coliform counts, increasing Lactobacillus amounts, and diminishing inflammation.

Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk

2019 ◽  
Vol 123 ◽  
pp. 286-297 ◽  
Author(s):  
Muhammad Shahid Riaz Rajoka ◽  
Haobin Zhao ◽  
Hafiza Mahreen Mehwish ◽  
Na Li ◽  
Yao Lu ◽  
...  
Keyword(s):  
Breast Milk ◽  
Culture Supernatant ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Cell Free Culture Supernatant

Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk

LWT ◽  
2018 ◽  
Vol 89 ◽  
pp. 638-647 ◽  
Author(s):  
Muhammad Shahid Riaz Rajoka ◽  
Mingliang Jin ◽  
Zhao Haobin ◽  
Qi Li ◽  
Dongyan Shao ◽  
...  
Keyword(s):  
Breast Milk ◽  
Functional Characterization ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Biotechnological Potential

Mechanisms for Lactobacillus rhamnosus treatment of intestinal infection by drug-resistant Escherichia coli

2020 ◽  
Vol 11 (5) ◽  
pp. 4428-4445 ◽  
Author(s):  
Na Li ◽  
Bing Pang ◽  
Junjun Li ◽  
Guanwen Liu ◽  
Xiaoguang Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Rhamnosus ◽  
Multiple Drug ◽  
Therapeutic Effects ◽  
Drug Resistant ◽  
Intestinal Infection ◽  
E Coli ◽  
Intestinal Integrity ◽  
Multiple Drug Resistant

Reducing the viability of pathogens may also play an important role for the therapeutic effects of Lactobacillus rhamnosus SHA113 against multiple-drug-resistant E. coli, as well as influencing on the intestinal integrity and functions of animals.

Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation

2021 ◽  
Author(s):  
Bing Pang ◽  
Han Jin ◽  
Ning Liao ◽  
Junjun Li ◽  
Chunmei Jiang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Breast Milk ◽  
Gut Microbiota ◽  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Gut Flora ◽  
Healthy Human

Gut microbiota imbalance is one of the major causes of ulcerative colitis (UC). L. rhamnosus SHA113 (LRS), a strain isolated from healthy human milk, influences the regulation of gut flora....

scholarly journals Sustainable, Alginate-Based Sensor for Detection of Escherichia coli in Human Breast Milk

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1145
Author(s):  
Nicholas Kikuchi ◽  
Margaret May ◽  
Matthew Zweber ◽  
Jerard Madamba ◽  
Craig Stephens ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Breast Milk ◽  
Pathogen Detection ◽  
Protein Extraction ◽  
Limit Of Detection ◽  
Pcr Amplification ◽  
Human Breast Milk ◽  
Blue Color ◽  
E Coli ◽  
The Cost

There are no existing affordable diagnostics for sensitive, rapid, and on-site detection of pathogens in milk. To this end, an on-site colorimetric-based sustainable assay has been developed and optimized using an L16 (54) Taguchi design to obtain results in hours without PCR amplification. To determine the level of Escherichia coli (E. coli) contamination, after induction with 150 µL of breast milk, the B-Per bacterial protein extraction kit was added to a solution containing an alginate-based microcapsule assay. Within this 3 mm spherical novel sensor design, X-Gal (5-Bromo-4-Chloro-3-Indolyl β-d-Galactopyranoside) was entrapped at a concentration of 2 mg/mL. The outward diffusing X-Gal was cleaved by β-galactosidase from E. coli and dimerized in the solution to yield a blue color after incubation at 40 °C. Color intensity was correlated with the level of E. coli contamination using a categorical scale. After an 8 h incubation period, a continuous imaging scale based on intensity normalization was used to determine a binary lower limit of detection (LOD), which corresponded to 102 colony forming unit per mL (CFU/mL) and above. The cost of the overall assay was estimated to be $0.81 per sample, well under the $3 benchmark for state-of-the-art immune-based test kits for pathogen detection in biofluids. Considering the reported binary LOD cutoff of 102 CFU/mL and above, this proposed hydrogel-based assay is suited to meet global requirements for screening breast milk or milk for pathogenic organisms of 104 CFU/mL, with a percentage of false positives to be determined in future efforts.

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