Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation

2021 ◽  
Author(s):  
Bing Pang ◽  
Han Jin ◽  
Ning Liao ◽  
Junjun Li ◽  
Chunmei Jiang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Breast Milk ◽  
Gut Microbiota ◽  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Gut Flora ◽  
Healthy Human

Gut microbiota imbalance is one of the major causes of ulcerative colitis (UC). L. rhamnosus SHA113 (LRS), a strain isolated from healthy human milk, influences the regulation of gut flora....

Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk

2019 ◽  
Vol 123 ◽  
pp. 286-297 ◽  
Author(s):  
Muhammad Shahid Riaz Rajoka ◽  
Haobin Zhao ◽  
Hafiza Mahreen Mehwish ◽  
Na Li ◽  
Yao Lu ◽  
...  
Keyword(s):  
Breast Milk ◽  
Culture Supernatant ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Cell Free Culture Supernatant

Human Breast Milk Contains Bovine lgG. Relationship to Infant Colic?

PEDIATRICS ◽  
1991 ◽  
Vol 87 (4) ◽  
pp. 439-444
Author(s):  
Patrick S. Clyne ◽  
Anthony Kulczycki
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Human Breast Milk ◽  
Human Breast ◽  
Cow's Milk ◽  
Milk Samples ◽  
Infant Colic ◽  
Β Lactoglobulin

Previous studies have suggested that an unidentified cow's milk protein, other than β-lactoglobulin and casein, might play a pathogenetic role in infant colic. Therefore, a radioimmunoassay was used to analyze human breast milk and infant formula samples for the presence of bovine IgG. Milk samples from 88 of the 97 mothers tested contained greater than 0.1 µg/mL of bovine IgG. In a study group of 59 mothers with infants in the colic-prone 2- to 17-week age group, the 29 mothers of colicky infants had higher levels of bovine IgG in their breast milk (median 0.42 µg/mL) than the 30 mothers of noncolicky infants (median 0.32 µg/mL) (P < .02). The highest concentrations of bovine IgG observed in human milk were 8.5 and 8.2 µg/mL. Most cow's milk-based infant formulas contained 0.6 to 6.4 µg/mL of bovine IgG, a concentration comparable with levels found in many human milk samples. The results suggest that appreciable quantities of bovine IgG are commonly present in human milk, that significantly higher levels are present in milk from mothers of colicky infants, and that bovine IgG may possibly be involved in the pathogenesis of infant colic.

scholarly journals Detectable Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Human Breast Milk of a Mildly Symptomatic Patient With Coronavirus Disease 2019 (COVID-19)

2020 ◽  
Author(s):  
Patrick C K Tam ◽  
Kathleen M Ly ◽  
Max L Kernich ◽  
Nicola Spurrier ◽  
Diana Lawrence ◽  
...  
Keyword(s):  
Case Report ◽  
Breast Milk ◽  
Severe Acute Respiratory Syndrome ◽  
Human Milk ◽  
Symptomatic Patient ◽  
Clinical Samples ◽  
Human Breast Milk ◽  
Causative Pathogen ◽  
Human Breast ◽  
Novel Coronavirus

Abstract SARS-CoV-2 is a novel coronavirus and causative pathogen to the pandemic illness COVID-19. Although RNA has been detected in various clinical samples, no reports to date have documented SARS-CoV-2 in human milk. This case report describes an actively breastfeeding patient with COVID-19 infection with detectable viral RNA in human milk.

scholarly journals Inhibitors of Complement Activity in Human Breast-Milk: A Proposed Hypothesis of Their Physiological Significance

1999 ◽  
Vol 8 (2) ◽  
pp. 69-75 ◽  
Author(s):  
Michael Oladipo Ogundele
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Complement Activation ◽  
Milk Fat ◽  
Physiological Significance ◽  
Human Breast Milk ◽  
Human Breast ◽  
Complement Activity ◽  
Milk Fat Globule ◽  
Fat Globule

Several natural components abundant in the fluid phase of human breast-milk have been shown to be inhibitors of complement activationin vitro, particularly the classical pathway. These include lysozyme, lactoferrin, lactalbumin alpha and other ligand chelators, complement regulator proteins and other specific soluble inhibitors of complement activation. Their physiological significance probably resides in their ability to restrictin vivocomplement activation to specialized (compartmentalized) sites on the cellular membrane structures in human milk, represented by the abundant surface area of the milk fat globule membranes. This would serve to prevent inflammatory-induced tissue damage of the delicate immature gastrointestinal tract of the newborn as well as the mammary gland itself. A number of recognized and potential inhibitors of complement activity in human milk and other biological fluids are hereby reviewed, with a proposal of their physiological significance.Abbreviations: HBM, human breast-milk; APC, alternative complement activation pathway; MAC, membrane attack complex (C5b-9); MFGM, milk fat globule membrane

scholarly journals COVID-19 mRNA vaccine is not detected in human milk

2021 ◽  
Author(s):  
Yarden Golan ◽  
Mary Prahl ◽  
Arianna Cassidy ◽  
Christine Y. Lin ◽  
Nadav Ahituv ◽  
...  
Keyword(s):  
Clinical Trials ◽  
High Risk ◽  
Breast Milk ◽  
Human Milk ◽  
Human Breast Milk ◽  
Human Breast ◽  
Phase 3 ◽  
Milk Samples

AbstractSeveral countries have recently approved the use of mRNA vaccines against COVID-19 under an emergency use authorization. However, no pregnant or lactating individuals were included in the Phase 3 clinical trials of these vaccines despite belonging to a group at high risk for severe complications of COVID-19 infection. We show here that the mRNA from anti-COVID BNT162b2 (Pfizer) and mRNA-1273 (Moderna) vaccines is not detected in human breast milk samples collected 4-48 hours post-vaccine. These results strengthen the recommendation of ABM and WHO that lactating individuals who receive the anti-COVID-19 mRNA-based vaccine should continue to breastfeed their infants uninterrupted.

scholarly journals Architecture Insight of Bifidobacterial α-L-Fucosidases

2021 ◽  
Author(s):  
José Antonio Curiel ◽  
Ángela Peirotén ◽  
José M. Landete ◽  
Ana Ruiz de la Bastida ◽  
Susana Langa ◽  
...  
Keyword(s):  
Breast Milk ◽  
Gut Microbiota ◽  
Early Life ◽  
Computational Analysis ◽  
Glycosyl Hydrolase ◽  
Human Breast Milk ◽  
Human Breast ◽  
Physicochemical Studies ◽  
Phylogenetic Lineages ◽  
Similarity Searches

Fucosylated carbohydrates and glycoproteins from human breast milk are essential for the development of the gut microbiota in early life because they are selectively metabolized by bifidobacteria. In this regard, α-L-fucosidases play a key role in this successful bifidobacterial colonization allowing the utilization of these substrates. Although a considerable number of α-L-fucosidases from bifidobacteria have been identified by computational analysis, only a few of them have been characterized. Hitherto, α-L-fucosidases are classified into 3 families, GH29, GH95 and GH151 based on their catalytic structure. However, bifidobacterial α-L-fucosidases belonging to a particular family show significant differences in their sequence. Because this fact could underlie distinct phylogenetic evolves, here extensive similarity searches and comparative analyses of the bifidobacterial α-L-fucosidases identified were carried out with the assistance of previous physicochemical studies available. This work reveals 4 and 2 paralogue bifidobacterial fucosidase groups within GH29 and GH95 families, respectively. Moreover, Bifidobacterium logum subsp. infantis species exhibited the greatest number of phylogenetic lineages in their fucosidases clustered in every family GH29, GH95 and GH151. Since α-L-fucosidases phylogenetically descended from other glycosyl hydrolase families, we hypothesized that could exhibit additional glycosidase activities other than fucosidase, raising the possibility about their application to transfucosylate other substrates than lactose in order to synthesis novel prebiotics.

scholarly journals Nordic study on human milk fortification in extremely preterm infants: a randomised controlled trial—the N-forte trial

BMJ Open ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. e053400
Author(s):  
Georg Bach Jensen ◽  
Fredrik Ahlsson ◽  
Magnus Domellöf ◽  
Anders Elfvin ◽  
Lars Naver ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Preterm Infants ◽  
Ethical Review ◽  
Human Breast Milk ◽  
Human Breast ◽  
Preterm Formula ◽  
Extremely Preterm ◽  
Extremely Preterm Infants ◽  
Randomised Controlled

IntroductionThe mortality rate of extremely low gestational age (ELGA) (born <gestational week 28+0) infants remains high, and severe infections and necrotising enterocolitis (NEC) are common causes of death. Preterm infants receiving human milk have lower incidence of sepsis and NEC than those fed a bovine milk-based preterm formula. Despite this, fully human milk fed ELGA infants most often have a significant intake of cow’s milk protein from bovine-based protein fortifier. The aim of this study is to evaluate whether the supplementation of human milk-based, as compared with bovine-based, nutrient fortifier reduces the prevalence of NEC, sepsis and mortality in ELGA infants exclusively fed with human milk.Methods and analysisA randomised-controlled multicentre trial comparing the effect of a human breast milk-based fortifier with a standard bovine protein-based fortifier in 222–322 ELGA infants fed human breast milk (mother’s own milk and/or donor milk). The infants will be randomised to either fortifier before reaching 100 mL/kg/day in oral feeds. The intervention, stratified by centre, will continue until the target postmenstrual week 34+0. The primary outcome is a composite of NEC, sepsis or death. Infants are characterised with comprehensive clinical and nutritional data collected prospectively from birth until hospital discharge. Stool, urine, blood and breast milk samples are collected for analyses in order to study underlying mechanisms. A follow-up focusing on neurological development and growth will be performed at 2 and 5.5 years of age. Health economic analyses will be made.Ethics and disseminationThe study is conducted according to ICH/GCP guidelines and is approved by the regional ethical review board in Linköping Sweden (Dnr 2018/193-31, Dnr 2018/384-32). Results will be presented at scientific meetings and published in peer-reviewed publications.Trial registration numberThe study was registered with ClinicalTrials.gov NCT03797157, 9 January 2019.

Lactobacillus rhamnosus from human breast milk shows therapeutic function against foodborne infection by multi-drug resistant Escherichia coli in mice

2020 ◽  
Vol 11 (1) ◽  
pp. 435-447 ◽  
Author(s):  
Na Li ◽  
Bing Pang ◽  
Guanwen Liu ◽  
Xixi Zhao ◽  
Xiaoguang Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Breast Milk ◽  
Therapeutic Efficacy ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Drug Resistant ◽  
Human Breast ◽  
E Coli ◽  
Therapeutic Function ◽  
Foodborne Infection

Lactobacillus rhamnosus shows higher therapeutic efficacy than antibiotic to treat drug-resistant E. coli infection in aspects of fast reducing coliform counts, increasing Lactobacillus amounts, and diminishing inflammation.

Characteristics of Human Breast Milk Based on Infant’s Gender

2019 ◽  
Vol 5 (1) ◽  
pp. 8-10
Author(s):  
Moudah Alhindi ◽  
Hadohum Musallam ◽  
Esam Elhefian
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Physicochemical Properties ◽  
Infant Formula ◽  
Human Breast Milk ◽  
Human Breast ◽  
Milk Samples ◽  
Complex Fluid ◽  
Infant Sex ◽  
Calcium And Magnesium

Human milk is a complex fluid, which contains a number of constituents such as fats, proteins and vitamins, in addition to other compounds. These nutrients are needed for infant protection against infections and diseases. This study was undertaken to evaluate some physicochemical properties and biochemical constituents content of human milk samples (based on the gender of the breast feeding infant). This includes acidity, density and viscosity, as well as lactose, protein, calcium and magnesium contents. Results showed that the highest value of viscosity as well as the highest percentages of protein and acidity were recorded for human breast milk for a boy (2.212 cP, 1.237% and 0.02% respectively) while close values for density and other biochemical contents were recorded for both types of samples. However, only the change in viscosity was found to be significant (p?0.05). Variations in some properties of human milk depending on the infant' sex could be of benefit for developing infant formula taking infant’s gender into consideration.

Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk

LWT ◽  
2018 ◽  
Vol 89 ◽  
pp. 638-647 ◽  
Author(s):  
Muhammad Shahid Riaz Rajoka ◽  
Mingliang Jin ◽  
Zhao Haobin ◽  
Qi Li ◽  
Dongyan Shao ◽  
...  
Keyword(s):  
Breast Milk ◽  
Functional Characterization ◽  
Lactobacillus Rhamnosus ◽  
Human Breast Milk ◽  
Human Breast ◽  
Biotechnological Potential
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