scholarly journals Phosphinate-containing rhodol and fluorescein scaffolds for the development of bioprobes

2019 ◽  
Vol 55 (42) ◽  
pp. 5962-5965 ◽  
Author(s):  
Yuan Fang ◽  
Gillian N. Good ◽  
Xinqi Zhou ◽  
Cliff I. Stains
Keyword(s):  

A series of phosphinate-containing rhodol and fluorescein dyes are disclosed.

Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.


2010 ◽  
Vol 345 (13) ◽  
pp. 1882-1890 ◽  
Author(s):  
Larisa N. Bogdanova ◽  
Nikolay O. Mchedlov-Petrossyan ◽  
Natalya A. Vodolazkaya ◽  
Alexander V. Lebed

2021 ◽  
Author(s):  
Anastasia Rogova ◽  
Marina Gerasimova ◽  
Felix Tomilin ◽  
Evgenia Slyusareva

1933 ◽  
Vol 37 (9) ◽  
pp. 1123-1133 ◽  
Author(s):  
H. F. Blum ◽  
C. R. Spealman
Keyword(s):  

2018 ◽  
Vol 9 (20) ◽  
pp. 2727-2732 ◽  
Author(s):  
Tao Tao ◽  
Ruiqing Wang ◽  
Hongjie Xu ◽  
Jie Yin ◽  
Xuesong Jiang

hPEA nanomicelles can encapsulate fluorescein dyes as a nanoreactor, leading to the fast photobleaching of dyes.


Sign in / Sign up

Export Citation Format

Share Document