Photochemistry of Fluorescein Dyes.

1933 ◽  
Vol 37 (9) ◽  
pp. 1123-1133 ◽  
Author(s):  
H. F. Blum ◽  
C. R. Spealman
Keyword(s):  
Fluorescein Dyes

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

The influence of β-cyclodextrin on acid–base and tautomeric equilibrium of fluorescein dyes in aqueous solution

2010 ◽  
Vol 345 (13) ◽  
pp. 1882-1890 ◽  
Author(s):  
Larisa N. Bogdanova ◽  
Nikolay O. Mchedlov-Petrossyan ◽  
Natalya A. Vodolazkaya ◽  
Alexander V. Lebed
Keyword(s):  
Aqueous Solution ◽  
Tautomeric Equilibrium ◽  
Acid Base ◽  
Fluorescein Dyes

Quantum chemical study of the spectral characteristics of fluorescein dyes bound to chitosan

2021 ◽  
Author(s):  
Anastasia Rogova ◽  
Marina Gerasimova ◽  
Felix Tomilin ◽  
Evgenia Slyusareva
Keyword(s):  
Quantum Chemical ◽  
Spectral Characteristics ◽  
Quantum Chemical Study ◽  
Chemical Study ◽  
Fluorescein Dyes

Hyperbranched poly(ether amine) nanomicelles as nanoreactors for the unexpected ultrafast photolysis of fluorescein dyes

2018 ◽  
Vol 9 (20) ◽  
pp. 2727-2732 ◽  
Author(s):  
Tao Tao ◽  
Ruiqing Wang ◽  
Hongjie Xu ◽  
Jie Yin ◽  
Xuesong Jiang
Keyword(s):  
Hyperbranched Poly ◽  
Fluorescein Dyes

hPEA nanomicelles can encapsulate fluorescein dyes as a nanoreactor, leading to the fast photobleaching of dyes.

Anomalous Kinetics of Depletion Zone Growth with One-Dimensional Geometry: Photobleaching Experiments and Simulations

2003 ◽  
Vol 790 ◽  
Author(s):  
Hailin Peng ◽  
Sung Hyun Park ◽  
Haim Taitelbaum ◽  
Raoul Kopelman
Keyword(s):  
Main Parameter ◽  
Depletion Zone ◽  
Previous Theory ◽  
Slow Increase ◽  
One Dimensional ◽  
Diffusion Limited ◽  
Simulation Results ◽  
Kinetics Of ◽  
Fluorescein Dyes ◽  
Zone Growth

ABSTRACTWe studied the kinetics of a diffusion-limited trapping reaction A + T → T in an effective one-dimensional geometry by experiments, supported by simulation calculations. The experiments are based on the photobleaching reaction of fluorescein dyes. The main parameter of interest is the θ-distance, which is related to the expansion of the depletion zone and can be measured directly from experiment. We found that θ-distance scales with t1/2 asymptotically, as predicted by previous theory. In addition, we found that the θ-distance increases faster than t1/2 inside the trap at early times, which has not been explored previously. Furthermore, there is a third, intermediate regime, with a slow increase of slope. Our experimental results are consistent with our simulation results.

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