Red phosphorus decorated graphene oxide nanosheets: label-free DNA detection

2020 ◽  
Vol 8 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Tapas Kumar Mandal ◽  
Yong Rok Lee ◽  
Nargish Parvin
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Intensity ◽  
Rapid Response ◽  
Label Free ◽  
Red Phosphorus ◽  
Graphene Oxide Nanosheets ◽  
Double Stranded Dna ◽  
Free Dna ◽  
Target Dna ◽  
Fluorescent Tags

f-RP@N-rGO NSs are promising structures for the selective sensing of target DNA molecules through a highly rapid response in fluorescence intensity toward single and double stranded DNA without the need of any fluorescent tags.

Label-Free Aptamer Fluorescence Determination of Trace Pb2+ Using AuPd Nanoalloy Probe as Catalyst

2013 ◽  
Vol 631-632 ◽  
pp. 18-21 ◽  
Author(s):  
Zhi Liang Jiang ◽  
Mei Ling Tang ◽  
Qing Ye Liu ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Water Samples ◽  
Catalytic Effect ◽  
Rhodamine 6G ◽  
Label Free ◽  
Double Stranded Dna ◽  
Fluorescence Determination

In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb2+ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb2+ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH2PO2, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF552nm) was linear to the concentration of Pb2+ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

An electrochemical biosensor based on a graphene oxide modified pencil graphite electrode for direct detection and discrimination of double-stranded DNA sequences

2020 ◽  
Vol 12 (37) ◽  
pp. 4541-4550
Author(s):  
Mehran Ahmadi ◽  
Fatemeh Ahour
Keyword(s):  
Graphene Oxide ◽  
Dna Sequences ◽  
Electrochemical Biosensor ◽  
Graphite Electrode ◽  
Direct Detection ◽  
Pencil Graphite Electrode ◽  
Label Free ◽  
Double Stranded Dna

A simple electrochemical biosensor using a GO modified PGE for label-free and direct detection of ds-DNA in plasmid samples without denaturation.

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing

2014 ◽  
Vol 60 ◽  
pp. 161-166 ◽  
Author(s):  
Zhao Zhang ◽  
Liqiang Luo ◽  
Guifang Chen ◽  
Yaping Ding ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

scholarly journals Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection

2015 ◽  
Vol 72 ◽  
pp. 313-319 ◽  
Author(s):  
Bing Li ◽  
Genhua Pan ◽  
Neil D. Avent ◽  
Roy B. Lowry ◽  
Tracey E. Madgett ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Dna Detection ◽  
Label Free ◽  
Graphene Electrode ◽  
Free Dna ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

2018 ◽  
Vol 10 (45) ◽  
pp. 5364-5371 ◽  
Author(s):  
Zhilin Hong ◽  
Genwang Chen ◽  
Shaoyang Yu ◽  
Rongfu Huang ◽  
Chunmei Fan
Keyword(s):  
Graphene Oxide ◽  
Carcinoembryonic Antigen ◽  
Modified Electrode ◽  
Signal Amplification ◽  
Dnase I ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Target Recycling

A label-free potentiometric aptasensing platform was designed for detection of carcinoembryonic antigen on a graphene oxide-modified electrode coupling with target recycling-assisted signal amplification.

Label-free detection of double-stranded DNA molecules with polyelectrolyte-modified capacitive field-effect sensors

2017 ◽  
Vol 84 (10) ◽  
Author(s):  
Thomas S. Bronder ◽  
Arshak Poghossian ◽  
Michael Keusgen ◽  
Michael J. Schöning
Keyword(s):  
Field Effect ◽  
Deoxyribonucleic Acid ◽  
Label Free ◽  
Electrical Detection ◽  
Dna Oligonucleotides ◽  
Double Stranded Dna ◽  
Polyelectrolyte Layer ◽  
Target Dna ◽  
Label Free Detection ◽  
Positively Charged

AbstractIn this study, polyelectrolyte-modified field-effect-based electrolyte-insulator-semiconductor (EIS) devices have been used for the label-free electrical detection of double-stranded deoxyribonucleic acid (dsDNA) molecules. The sensor-chip functionalization with a positively charged polyelectrolyte layer provides the possibility of direct adsorptive binding of negatively charged target DNA oligonucleotides onto the SiO

Sign in / Sign up

Export Citation Format

Share Document