A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

2018 ◽  
Vol 10 (45) ◽  
pp. 5364-5371 ◽  
Author(s):  
Zhilin Hong ◽  
Genwang Chen ◽  
Shaoyang Yu ◽  
Rongfu Huang ◽  
Chunmei Fan
Keyword(s):  
Graphene Oxide ◽  
Carcinoembryonic Antigen ◽  
Modified Electrode ◽  
Signal Amplification ◽  
Dnase I ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Target Recycling

A label-free potentiometric aptasensing platform was designed for detection of carcinoembryonic antigen on a graphene oxide-modified electrode coupling with target recycling-assisted signal amplification.

Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres

2015 ◽  
Vol 51 (11) ◽  
pp. 2156-2158 ◽  
Author(s):  
Yi Xie ◽  
Xiaoyan Lin ◽  
Yishun Huang ◽  
Rujun Pan ◽  
Zhi Zhu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dnase I ◽  
Dna Probes ◽  
Selective Detection ◽  
Protective Properties ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Nuclease Digestion

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA.

Gold nanoparticle decorated polypyrrole/graphene oxide nanosheets as a modified electrode for simultaneous determination of ascorbic acid, dopamine and uric acid

2020 ◽  
Vol 44 (12) ◽  
pp. 4916-4926 ◽  
Author(s):  
Chaoli Tan ◽  
Jie Zhao ◽  
Peng Sun ◽  
Wei Zheng ◽  
Guofeng Cui
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Electrochemical Performance ◽  
Simultaneous Determination ◽  
Gold Nanoparticle ◽  
Modified Electrode ◽  
Graphene Oxide Nanosheets

AuNPs, GO and PPy are combined effectively to form a novel composite showing a huge enhancement in electrochemical performance compared to the single materials.

Label-free and dual-amplified electrochemical detection of Hg2+ based on self-assembled DNA nanostructures and target-triggered exonuclease cleavage activity

2016 ◽  
Vol 40 (8) ◽  
pp. 6686-6691 ◽  
Author(s):  
Ting Bao ◽  
Wei Wen ◽  
Lei Shu ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Electrochemical Detection ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Dna Nanostructures ◽  
Label Free ◽  
Target Recycling ◽  
Cleavage Activity ◽  
Self Assembled ◽  
Exo Iii

An electrochemical biosensor for Hg2+ detection via HCR and Hg2+-triggered Exo III-assisted target recycling for signal amplification.

scholarly journals New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1256
Author(s):  
Pingping Xu ◽  
Wenpo Feng ◽  
Mei Wang ◽  
Ling Zhang ◽  
Gaofeng Liang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Carcinoembryonic Antigen ◽  
Au Nanoparticle ◽  
Functionalized Graphene ◽  
Label Free ◽  
Functionalized Graphene Oxide ◽  
Amine Functionalized ◽  
Label Free Detection ◽  
Sandwich Type ◽  
Fan Blade

The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH2-GO) were the label of secondary antibodies (Ab2), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH2-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab2) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab2/PdAuCu NPs/NH2-GO catalyzed the reduction of H2O2 in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL−1 and a linear detection range from 0.1 pg mL−1 to 200 ng mL−1. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.

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