scholarly journals Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection

2015 ◽  
Vol 72 ◽  
pp. 313-319 ◽  
Author(s):  
Bing Li ◽  
Genhua Pan ◽  
Neil D. Avent ◽  
Roy B. Lowry ◽  
Tracey E. Madgett ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Dna Detection ◽  
Label Free ◽  
Graphene Electrode ◽  
Free Dna ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A label-free and ultrasensitive DNA impedimetric sensor with enzymatic and electrical dual-amplification

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4175-4179 ◽  
Author(s):  
Shuo-Hui Cao ◽  
Lun-Hui Li ◽  
Wen-Yin Wei ◽  
Ye Feng ◽  
Wen-Long Jiang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Dna Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide ◽  
Dual Amplification

Combined with exonuclease III, electrochemically reduced graphene oxide could be a desirable platform to amplify impedimetric changes for ultrasensitive DNA detection.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing

2014 ◽  
Vol 60 ◽  
pp. 161-166 ◽  
Author(s):  
Zhao Zhang ◽  
Liqiang Luo ◽  
Guifang Chen ◽  
Yaping Ding ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

Gold nanoparticles and reduced graphene oxide-amplified label-free DNA biosensor for dasatinib detection

2018 ◽  
Vol 42 (19) ◽  
pp. 16378-16383 ◽  
Author(s):  
Fahimeh Tahernejad-Javazmi ◽  
Mehdi Shabani-Nooshabadi ◽  
Hassan Karimi-Maleh
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Adverse Effects ◽  
Reduced Graphene Oxide ◽  
The Other ◽  
Myelogenous Leukemia ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

Dasatinib or sprycel is an anticancer drug for treatment of chronic myelogenous leukemia, prostate cancer, and some of the other cancers with several adverse effects.

scholarly journals Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Gen Liu ◽  
Wei Ma ◽  
Yan Luo ◽  
Deng-ming Sun ◽  
Shuang Shao
Keyword(s):  
Methylene Blue ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Composite Film ◽  
Simultaneous Determination ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽  
2021 ◽  
Author(s):  
Xinke Liu ◽  
Lu-Yin Lin ◽  
Fu-Yen Tseng ◽  
Yu-Cheng Tan ◽  
Jian Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Matrix Metalloproteinase 1 ◽  
Reduced Graphene ◽  
P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

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