Hydrogen-producing hyperthermophilic bacteria synthesized size-controllable fine gold nanoparticles with excellence for eradicating biofilm and antibacterial applications

2018 ◽  
Vol 6 (28) ◽  
pp. 4602-4609 ◽  
Author(s):  
Wei Bing ◽  
Hanjun Sun ◽  
Faming Wang ◽  
Yanqiu Song ◽  
Jinsong Ren
Keyword(s):  
Gold Nanoparticles ◽  
Peroxidase Activity ◽  
Environment Friendly ◽  
Hyperthermophilic Bacteria ◽  
Ph Range ◽  
Controllable Preparation ◽  
Fine Gold

An environment-friendly strategy for the controllable preparation of AuNPs is presented, which exhibited high peroxidase activity over a broad pH range.

Preparation of Highly Stable Oligo(ethylene glycol) Derivatives-Functionalized Gold Nanoparticles and Their Application in LSPR-Based Detection of PSA/ACT Complex

2007 ◽  
Vol 7 (11) ◽  
pp. 3754-3757 ◽  
Author(s):  
Cuong Cao ◽  
Sang Jun Sim
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Particles ◽  
Sandwich Immunoassay ◽  
Immunoassay Technique ◽  
Gold Nanoparticle Aggregation ◽  
Wide Ph Range ◽  
Complex Detection ◽  
Ethylene Glycol Derivatives ◽  
Ph Range ◽  
First Time

A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

scholarly journals Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate

2012 ◽  
Vol 14 (4) ◽  
pp. 57-60
Author(s):  
Peng Zhang ◽  
Chunli Liu ◽  
Shanshan Song ◽  
Chifang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Enzyme Activity ◽  
High Activity ◽  
Immobilized Enzyme ◽  
Absorption Method ◽  
Horse Radish Peroxidase ◽  
Highly Active ◽  
Peroxidase Enzyme ◽  
Novel Method ◽  
Controllable Preparation

Abstract A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme.

Ultrasensitive and selective colorimetric detection of acetamiprid pesticide based on the enhanced peroxidase-like activity of gold nanoparticles

2017 ◽  
Vol 9 (37) ◽  
pp. 5484-5493 ◽  
Author(s):  
Wenping Yang ◽  
Yuangen Wu ◽  
Han Tao ◽  
Jing Zhao ◽  
Huayun Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Peroxidase Activity ◽  
Colorimetric Detection ◽  
Ultrasensitive Detection ◽  
Colorimetric Aptasensor

A colorimetric aptasensor based on the peroxidase activity of AuNPs has been proposed for the rapid and ultrasensitive detection of acetamiprid.

Release of peroxidase from soybean hypocotyl cell walls by Sclerotium rolfsii culture filtrates

1974 ◽  
Vol 52 (1) ◽  
pp. 265-271 ◽  
Author(s):  
Neal M. Barnett
Keyword(s):  
Culture Filtrate ◽  
Peroxidase Activity ◽  
Cell Walls ◽  
Hydrolytic Enzymes ◽  
Sclerotium Rolfsii ◽  
Release Reaction ◽  
Culture Filtrates ◽  
Ph Range

Up to 24% of the peroxidase of purified cell walls of soybean hypocotyls was released by incubation of cell walls with hydrolytic enzymes secreted by the fungus Sclerotium rolfsii. This estimate is based on comparison of peroxidase activity recovered in the medium with peroxidase activity in unincubated cell walls, estimated by a new assay. The peroxidase-release reaction occurs at 0 °C at half the rate at 30 °C. The peroxidase-release reaction occurs almost equally fast in the pH range of 3.5 to 8.0. The release of peroxidase from cell walls cannot be attributed solely to arabanase, polygalacturonase, or cellulase in the culture filtrate, although on Sephadex G-75 chromatography these activities overlap the peroxidase-releasing activity. Culture filtrate released less than 5% of the hydroxyproline protein of the cell walls.

scholarly journals Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2144 ◽  
Author(s):  
Yan-Mei Sheng ◽  
Jian Liang ◽  
Jing Xie
Keyword(s):  
Gold Nanoparticles ◽  
Food Safety ◽  
Bovine Serum Albumin ◽  
Peroxidase Activity ◽  
Limit Of Detection ◽  
High Accuracy ◽  
Important Food ◽  
Bovine Serum Albumin Conjugate ◽  
Aptamer Assay

Tetracycline residue in honey has become an increasingly important food safety problem. In this work, an ultrasensitive gold nanoparticles (AuNPs)-linked aptamer assay was developed to determine the tetracycline residue in honey. First, a tetracycline–bovine serum albumin conjugate coating was applied to a microplate. Then, with the incubation of AuNPs-linked aptamer, the fixed tetracycline in the microplate competed for the limited aptamer with the free tetracycline in the sample. Higher amounts of free tetracycline in the sample were associated with more competitive binding of aptamer-AuNPs, and the aptamer-AuNPs binding with tetracycline-BSA was lower. Finally, as a kind of nanozyme, AuNPs exhibited peroxidase activity and oxidized 3,3′,5,5′-tetramethylbenzidine, transforming it from colorless to blue, and achieving the measurement at 652 nm. The analytical performance—including linearity, limit of detection, selectivity, precision, repeatability, and accuracy—has been investigated. It was successfully applied to the determination of tetracycline in honey samples with high accuracy and sensitivity.

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