Preparation of Highly Stable Oligo(ethylene glycol) Derivatives-Functionalized Gold Nanoparticles and Their Application in LSPR-Based Detection of PSA/ACT Complex

2007 ◽  
Vol 7 (11) ◽  
pp. 3754-3757 ◽  
Author(s):  
Cuong Cao ◽  
Sang Jun Sim
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Particles ◽  
Sandwich Immunoassay ◽  
Immunoassay Technique ◽  
Gold Nanoparticle Aggregation ◽  
Wide Ph Range ◽  
Complex Detection ◽  
Ethylene Glycol Derivatives ◽  
Ph Range ◽  
First Time

A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

Preparation of Highly Stable Oligo(ethylene glycol) Derivatives-Functionalized Gold Nanoparticles and Their Application in LSPR-Based Detection of PSA/ACT Complex

2007 ◽  
Vol 7 (11) ◽  
pp. 3754-3757 ◽  
Author(s):  
Cuong Cao ◽  
Sang Jun Sim
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Particles ◽  
Sandwich Immunoassay ◽  
Immunoassay Technique ◽  
Gold Nanoparticle Aggregation ◽  
Wide Ph Range ◽  
Complex Detection ◽  
Ethylene Glycol Derivatives ◽  
Ph Range ◽  
First Time

A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

Peroxidase-like activity of gold nanoparticles stabilized by hyperbranched polyglycidol derivatives over a wide pH range

2015 ◽  
Vol 26 (49) ◽  
pp. 495101 ◽  
Author(s):  
Marcin Drozd ◽  
Mariusz Pietrzak ◽  
Paweł Parzuchowski ◽  
Marta Mazurkiewicz-Pawlicka ◽  
Elżbieta Malinowska
Keyword(s):  
Gold Nanoparticles ◽  
Wide Ph Range ◽  
Ph Range

scholarly journals Partially hydroxylated ultrathin iridium nanosheets as efficient electrocatalysts for water splitting

2020 ◽  
Vol 7 (8) ◽  
pp. 1340-1348
Author(s):  
Zifang Cheng ◽  
Bolong Huang ◽  
Yecan Pi ◽  
Leigang Li ◽  
Qi Shao ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Water Splitting ◽  
Density Functional ◽  
Bond Cleavage ◽  
Critical Role ◽  
Density Functional Theory Calculations ◽  
Wide Ph Range ◽  
Initial Activation ◽  
Ph Range ◽  
First Time

Abstract Ultrathin two-dimensional (2D) materials have attracted considerable attention for their unique physicochemical properties and promising applications; however, preparation of freestanding ultrathin 2D noble metal remains a significant challenge. Here, for the first time, we report use of a wet-chemical method to synthesize partially hydroxylated ultrathin Ir nanosheets (Ir-NSs) of only five to six atomic layers’ thickness. Detailed analysis indicates that the growth confinement effect of carbon monoxide and the partially hydroxylated surface play a critical role in formation of the ultrathin structure. The ultrathin Ir-NSs exhibit excellent performance for both the hydrogen evolution reaction and oxygen evolution reaction in a wide pH range, outperforming the state-of-the-art Pt/C and IrO2, respectively. Density-functional theory calculations reveal that the partial hydroxylation not only enhances the surface electron transfer between Ir-sites and intermediate O-species, but also guarantees efficient initial activation of bond cleavage of H-O-H for first-step H2O splitting. This, ultimately, breaks through barriers to full water splitting, with efficient electron transfer essentially maintained.

AuNP based selective colorimetric sensor for cysteine at a wide pH range: investigation of capping molecule structure on the colorimetric sensing and catalytic properties

RSC Advances ◽  
2014 ◽  
Vol 4 (35) ◽  
pp. 18467-18472 ◽  
Author(s):  
V. Vinod Kumar ◽  
Savarimuthu Philip Anthony
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Properties ◽  
Colorimetric Sensor ◽  
Colorimetric Sensing ◽  
Molecule Structure ◽  
Wide Ph Range ◽  
Ph Range

Gold nanoparticles (AuNPs) stabilized with different surfactants, SDS, PEG, PVA, PVP, PSS and T-80, were synthesized and explored for cysteine colorimetric sensing and catalytic properties.

Synthesis of a silymarin-gold nanoparticle conjugate and analysis of its liver-protecting activity

2021 ◽  
Vol 22 ◽  
Author(s):  
Sergey Staroverov ◽  
Sergey Kozlov ◽  
Alexander Fomin ◽  
Konstantib Gabalov ◽  
Alexey Volkov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Glutathione Depletion ◽  
Laboratory Animals ◽  
Experimental Hepatitis ◽  
Acute Liver Disease ◽  
The Mean ◽  
Extent Of Damage ◽  
First Time

Background: The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction: Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold nanoparticles (AuNPs) as reagents. The study considered whether it was possible to use the silymarin-AuNP conjugate as a potential liver-protecting drug. Method: AuNPs were conjugated to Sil and examine the liver-protecting activity of the conjugate. Experimental hepatitis and hepatocyte cytolysis after carbon tetrachloride actionwere used as a model system, and the experiments were conducted on laboratory animals. Result: For the first time, silymarin was conjugated to colloidal gold nanoparticles (AuNPs). Electron microscopy showed that the resultant preparations were monodisperse and that the mean conjugate diameter was 18–30 nm ± 0.5 nm (mean diameter of the native nanoparticles, 15 ± 0.5 nm). In experimental hepatitis in mice, conjugate administration interfered with glutathione depletion in hepatocytes in response to carbon tetrachloride was conducive to an increase in energy metabolism, and stimulated the monocyte–macrophage function of the liver. The results were confirmed by the high respiratory activity of the hepatocytes in cell culture. Conclusion: We conclude that the silymarin-AuNP conjugate holds promise as a liver-protecting agent in acute liver disease caused by carbon tetrachloride poisoning.

Polarographic and spectrophotometric behaviour of some N-p-phenyl substituted benzamidines

1991 ◽  
Vol 56 (12) ◽  
pp. 2791-2799 ◽  
Author(s):  
Juan A. Squella ◽  
Luis J. Nuñez-Vergara ◽  
Hernan Rodríguez ◽  
Amelia Márquez ◽  
Jose M. Rodríguez-Mellado ◽  
...  
Keyword(s):  
Spectrophotometric Study ◽  
Absorption Bands ◽  
Electrochemical Parameters ◽  
Wide Ph Range ◽  
Group A ◽  
Substituent Constants ◽  
Ph Range ◽  
The Waves

Five N-p-phenyl substituted benzamidines were studied by DC and DP polarography in a wide pH range. Coulometric results show that the overall processes are four-electron reductions. Logarithmic analysis of the waves indicate that the process are irreversible. The influence of the pH on the polarographic parameters was also studied. A UV spectrophotometric study was performed in the pH range 2-13. In basic media some variations in the absorption bands were observed due to the dissociation of the amidine group. A determination of the pK values was made by deconvolution of the spectra. Correlations of both the electrochemical parameters and spectrophotometric pK values with the Hammett substituent constants were obtained.

High degradation efficiency of sulfamethazine with the dual-reaction-center Fe-Mn-SiO2 Fenton-like nanocatalyst in a wide pH range

2021 ◽  
Author(s):  
Manoj Kumar Panjwani ◽  
Qing Wang ◽  
Yueming Ma ◽  
Yuxuan Lin ◽  
Feng Xiao ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Reaction Center ◽  
Degradation Efficiency ◽  
Heterogeneous Fenton ◽  
Wide Ph Range ◽  
Ph Range

The development of a heterogeneous Fenton-like catalyst, possessing high degradation efficiency in a wide pH range, is crucial for wastewater treatment. The Fe-Mn-SiO2 catalyst was designed, and prepared by a...

scholarly journals Phosphatidylserine-Gold Nanoparticles (PS-AuNP) Induce Prostate and Breast Cancer Cell Apoptosis

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1094
Author(s):  
Allan Radaic ◽  
Nam E. Joo ◽  
Soo-Hwan Jeong ◽  
Seong-II Yoo ◽  
Nicholas Kotov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Biomedical Applications ◽  
Therapeutic Potential ◽  
Control Treatment ◽  
Track Record ◽  
Males And Females ◽  
Breast And Prostate Cancer ◽  
First Time

Prostate and breast cancer are the current leading causes of new cancer cases in males and females, respectively. Phosphatidylserine (PS) is an essential lipid that mediates macrophage efferocytosis and is dysregulated in tumors. Therefore, developing therapies that selectively restore PS may be a potential therapeutic approach for carcinogenesis. Among the nanomedicine strategies for delivering PS, biocompatible gold nanoparticles (AuNPs) have an extensive track record in biomedical applications. In this study, we synthesized biomimetic phosphatidylserine-caped gold nanoparticles (PS-AuNPs) and tested their anticancer potential in breast and prostate cancer cells in vitro. We found that both cell lines exhibited changes in cell morphology indicative of apoptosis. After evaluating for histone-associated DNA fragments, a hallmark of apoptosis, we found significant increases in DNA fragmentation upon PS-AuNP treatment compared to the control treatment. These findings demonstrate the use of phosphatidylserine coupled with gold nanoparticles as a potential treatment for prostate and breast cancer. To the best of our knowledge, this is the first time that a phosphatidylserine-capped AuNP has been examined for its therapeutic potential in cancer therapy.

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