scholarly journals 3D printed fittings and fluidic modules for customizable droplet generators

RSC Advances ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 2822-2828 ◽  
Author(s):  
Sindhu Vijayan ◽  
Michinao Hashimoto
Keyword(s):  
Rapid Method ◽  
Simple Method ◽  
3D Printed ◽  
Droplet Generators

We developed a rapid method to prototype axisymmetric droplet generators using 3D printed fittings and commercially available components. This simple method allowed generating simple and complex emulsions of varying sizes and configurations.

scholarly journals A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher

2020 ◽  
Author(s):  
Amanda C. Daulagala ◽  
John Yost ◽  
Amirreza Yeganegi ◽  
William J. Richardson ◽  
Michael J. Yost ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mechanical Strain ◽  
Simple Method ◽  
Cell Monolayers ◽  
3D Printed ◽  
Epithelial Cell Monolayers

scholarly journals A Simple Method for Separating Serum Lipoproteins by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 362-365 ◽  
Author(s):  
Margaret J Fletcher ◽  
Maria H Styliou
Keyword(s):  
Lipid Metabolism ◽  
Cellulose Acetate ◽  
Rapid Method ◽  
Serum Lipoproteins ◽  
Simple Method

Abstract A simple, rapid method is described for separating the serum lipoproteins into clear, discrete, and reproducible bands by electrophoresis on cellulose acetate. Sera of patients with primary or secondary hyperlipidemias show patterns reflecting changes in lipid metabolism. The method would be appropriate for use in screening surveys.

scholarly journals Microfluidic droplet generation based on non-embedded co-flow-focusing using 3D printed nozzle

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Adrien Dewandre ◽  
Javier Rivero-Rodriguez ◽  
Youen Vitry ◽  
Benjamin Sobac ◽  
Benoit Scheid
Keyword(s):  
Stokes Equations ◽  
Axisymmetric Flow ◽  
Droplet Generation ◽  
Droplet Radius ◽  
Planar Geometry ◽  
Flow Focusing ◽  
Glass Capillary ◽  
New Device ◽  
3D Printed ◽  
Droplet Generators

AbstractMost commercial microfluidic droplet generators rely on the planar flow-focusing configuration implemented in polymer or glass chips. The planar geometry, however, suffers from many limitations and drawbacks, such as the need of specific coatings or the use of dedicated surfactants, depending on the fluids in play. On the contrary, and thanks to their axisymmetric geometry, glass capillary-based droplet generators are a priori not fluid-dependent. Nevertheless, they have never reached the market because their assembly requires fastidious and not scalable fabrication techniques. Here we present a new device, called Raydrop, based on the alignment of two capillaries immersed in a pressurized chamber containing the continuous phase. The dispersed phase exits one of the capillaries through a 3D-printed nozzle placed in front of the extraction capillary for collecting the droplets. This non-embedded implementation of an axisymmetric flow-focusing is referred to non-embedded co-flow-focusing configuration. Experimental results demonstrate the universality of the device in terms of the variety of fluids that can be emulsified, as well as the range of droplet radii that can be obtained, without neither the need of surfactant nor coating. Additionally, numerical computations of the Navier-Stokes equations based on the quasi-steadiness assumption allow to provide an explanation to the underlying mechanism behind the drop formation and the mechanism of the dripping to jetting transition. Excellent predictions were also obtained for the droplet radius, as well as for the dripping-jetting transition, when varying the geometrical and fluid parameters, showing the ability of this configuration to enventually enhance the dripping regime. The monodispersity ensured by the dripping regime, the robustness of the fabrication technique, the optimization capabilities from the numerical modelling and the universality of the configuration confer to the Raydrop technology a very high potential in the race towards high-throughput droplet generation processes.

Simple Method for Concentration of Cerebrospinal Fluid for Protein Electrophoresis

1965 ◽  
Vol 11 (12) ◽  
pp. 1068-1069 ◽  
Author(s):  
Stanley Burrows
Keyword(s):  
Cerebrospinal Fluid ◽  
Rapid Method ◽  
Protein Electrophoresis ◽  
Protein Solutions ◽  
Simple Method ◽  
Dextran Solution

Abstract A rapid method for concentrating protein solutions is described. It utilizes dialysis against a dextran solution under vacuum.

scholarly journals A Facile Method to Fabricate Anisotropic Extracellular Matrix with 3D Printing Topological Microfibers

Materials ◽  
2019 ◽  
Vol 12 (23) ◽  
pp. 3944 ◽  
Author(s):  
Zhen Gu ◽  
Zili Gao ◽  
Wenli Liu ◽  
Yongqiang Wen ◽  
Qi Gu
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Mechanical Characteristics ◽  
Fiber Direction ◽  
Gelatin Matrix ◽  
Simple Method ◽  
Elongation At Break ◽  
Anisotropic Mechanical Properties ◽  
The Matrix ◽  
3D Printed

Natural tissues and organs have different requirements regarding the mechanical characteristics of response. It is still a challenge to achieve biomaterials with anisotropic mechanical properties using an extracellular matrix with biological activity. We have improved the ductility and modulus of the gelatin matrix using 3D printed gelatin microfibers with different concentrations and topologies and, at the same, time achieved anisotropic mechanical properties. We successfully printed flat microfibers using partially cross-linked gelatin. We modified the 10% (w/v) gelatin matrix with microfibers consisting of a gelatin concentration of 14% (w/v), increasing the modulus to about three times and the elongation at break by 39% in parallel with the fiber direction. At the same time, it is found that the microfiber topology can effectively change the matrix ductility, and changing the modulus of the gelatin used in the microfiber can effectively change the matrix modulus. These findings provide a simple method for obtaining active biological materials that are closer to a physiological environment.

scholarly journals Selective metallic coating of 3D-printed microstructures on flexible substrates

RSC Advances ◽  
2017 ◽  
Vol 7 (81) ◽  
pp. 51663-51669 ◽  
Author(s):  
Kuan-Ming Huang ◽  
Shang-Chen Tsai ◽  
Yu-Kuan Lee ◽  
Cheng-Kai Yuan ◽  
Yu-Ching Chang ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Metallic Coating ◽  
Flexible Substrates ◽  
Pdms Substrate ◽  
Simple Method ◽  
3D Printed

In this work, a simple method was developed to fabricate micron scale three-dimensional (3D) conductive objects on a flexible PDMS substrate.

scholarly journals Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration

2020 ◽  
Vol 5 (04) ◽  
pp. 54-59
Author(s):  
Nazia Begum ◽  
Kandavalli Manipriya ◽  
Rahathunnisa Begum ◽  
Veeresh B
Keyword(s):  
Estrous Cycle ◽  
Rapid Method ◽  
Crystal Violet ◽  
Vaginal Smear ◽  
Simple Method ◽  
Ovarian Morphology ◽  
Female Wistar Rats ◽  
Vaginal Cytology ◽  
Histomorphological Changes ◽  
Hormonal Levels

Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology and reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination in rats using crystal violet. With this technique, the identification of stages can be done even on the next day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the estrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works related to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the estrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet and to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.

A Simple and Rapid Method for Reducing Radiocesium Concentrations in Wild Mushrooms (Cantharellus and Boletus) in the Course of Cooking

2016 ◽  
Vol 79 (11) ◽  
pp. 1995-1999 ◽  
Author(s):  
GEORG STEINHAUSER ◽  
VERONIKA STEINHAUSER
Keyword(s):  
Rapid Method ◽  
Radioactive Cesium ◽  
Activity Levels ◽  
Nuclear Accidents ◽  
Wild Mushrooms ◽  
Simple Method ◽  
Lower Affinity ◽  
Boletus Edulis ◽  
High Fraction ◽  
High Concentrations

ABSTRACT Many species of mushrooms are known accumulators of radioactive cesium (137Cs and 134Cs). Even years and decades after major nuclear accidents, especially those at Chernobyl and Fukushima, mushrooms exhibit high concentrations of these radionuclides. We investigated a simple method for reducing the activity of radiocesium in wild mushrooms (chanterelles, Cantharellus cibarius; and boleti, Boletus edulis) during cooking. The juice generated while cooking mushrooms contains a relatively high fraction of the total cesium. The amount of juice can be increased by washing the mushrooms with water prior to cooking. By removing the juice, up to 29% of the radiocesium can be easily removed from chanterelles. Because boleti have a lower affinity for cesium, activity levels were lower in boleti than in chanterelles. The fraction of radiocesium in the juice was lower in boleti than in chanterelles.

A rapid method for the assessment of the macromycota. The fungal community of an evergreen cloud forest as an example

1998 ◽  
Vol 76 (4) ◽  
pp. 596-601
Author(s):  
R Guevara ◽  
R Dirzo
Keyword(s):  
Rapid Method ◽  
Fungal Community ◽  
Fungal Diversity ◽  
Cloud Forest ◽  
Simple Method

The macromycota of an evergreen cloud forest was described using a simple method, intended to minimize taxonomic work but still provide an accurate account of diversity. The method showed that the fungal community in the area is spatially structured and that area sampled limited the recording of fungal richness in this study. Parameters derived from the Clench equation suggest that an area of 1 ha will maximize the proportion of recorded taxa and minimize sampling effort.Key words: fungal diversity, macrofungal communities, tropical mycoecology, El Triunfo.

A Simple Method for the Detection of Benzo[a]pyrene in Smoked Foods

1964 ◽  
Vol 47 (5) ◽  
pp. 894-897
Author(s):  
C Genest ◽  
D M Smith
Keyword(s):  
Thin Layer ◽  
Dimethyl Sulfoxide ◽  
Ultraviolet Light ◽  
Rapid Method ◽  
Small Volume ◽  
Simple Method ◽  
Thin Layer Chromatographic Plate ◽  
Chromatographic Plate ◽  
Benzene Extract

Abstract Benzo[a]pyrene can be used as an index of carcinogens in smoke. A simple and rapid method has been developed for determining this substance. The dried food was extracted with ra-hexane, the hexane was then extracted with dimethyl sulfoxide, and the polynuclear hydrocarbon was displaced from this solvent into benzene by water. The benzene extract was reduced to a small volume and an aliquot was spotted on a thin-layer chromatographic plate beside spots of a standard benzo[a]pyrene solution. The plate was chroma tographed with 2,2,4-trimethyl pentane:benzene (97:3). When observed under ultraviolet light at 3660 Å, 0.002 μg of pure benzo[a]pyrene could be found and the addition of 0.01—0.05 ppm to unsmoked foods could be detected. No smoked food examined contained this level of benzo-[a]pyrene.

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