A rapid method for the assessment of the macromycota. The fungal community of an evergreen cloud forest as an example

1998 ◽  
Vol 76 (4) ◽  
pp. 596-601
Author(s):  
R Guevara ◽  
R Dirzo
Keyword(s):  
Rapid Method ◽  
Fungal Community ◽  
Fungal Diversity ◽  
Cloud Forest ◽  
Simple Method

The macromycota of an evergreen cloud forest was described using a simple method, intended to minimize taxonomic work but still provide an accurate account of diversity. The method showed that the fungal community in the area is spatially structured and that area sampled limited the recording of fungal richness in this study. Parameters derived from the Clench equation suggest that an area of 1 ha will maximize the proportion of recorded taxa and minimize sampling effort.Key words: fungal diversity, macrofungal communities, tropical mycoecology, El Triunfo.

scholarly journals Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota

2020 ◽  
Author(s):  
Shashank Gupta ◽  
Mathis H. Hjelmsø ◽  
Jenni Lehtimäki ◽  
Xuanji Li ◽  
Martin Steen Mortensen ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Fungal Community ◽  
Fungal Diversity ◽  
Environmental Exposures ◽  
Amplicon Sequencing ◽  
Living Environment ◽  
Crucial Importance ◽  
Healthy Development ◽  
Airway Microbiome ◽  
Sampling Season

Abstract Background From early life children are exposed to a multitude of environmental exposures, which may be of crucial importance for a healthy development. Here, the environmental microbiota may be of particular interest as it represents the interface between environmental factors and the child. As infants in modern societies spend a considerable amount of time indoors, we hypothesize that the indoor bed dust microbiota might be an important factor for the child and for the colonization of the early airway microbiome. To explore this hypothesis, we analysed the influence of environmental exposures on 577 dust samples from children’s beds (age 6 months) together with 542 airway samples (age 3 months) from the Copenhagen Prospective Studies on Asthma in Childhood 2010 cohort. Results The bed dust and airway microbiota were both profiled with bacterial amplicon sequencing while also fungal community was profiled from bed dust. Bacterial and fungal diversity in the dust was positively correlated. We observed that bacterial bed dust microbiota was influenced by multiple environmental factors, such as type of home (house or apartment), rural or urban living environment and pets (cat and/or dog), whereas fungal bed dust microbiota was majorly influenced by the sampling season. We further observed minor associations between bed dust and airway microbiota compositions among infants, but no evidence of transfer of individual taxa between the two departments. Conclusions Our finding demonstrate that bed dust microbiota is influenced by environmental exposures and could represent an interface between environment and child.

scholarly journals Diversity and Structure of Soil Fungal Communities across Experimental Everglades Tree Islands

Diversity ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 324
Author(s):  
Brianna K. Almeida ◽  
Michael S. Ross ◽  
Susana L. Stoffella ◽  
Jay P. Sah ◽  
Eric Cline ◽  
...  
Keyword(s):  
Water Level ◽  
Fungal Community ◽  
Plant Pathogen ◽  
Fungal Diversity ◽  
Fungal Communities ◽  
Tree Islands ◽  
Fungal Community Composition ◽  
Everglades Tree Islands ◽  
And Function ◽  
Early Restoration

Fungi play prominent roles in ecosystem services (e.g., nutrient cycling, decomposition) and thus have increasingly garnered attention in restoration ecology. However, it is unclear how most management decisions impact fungal communities, making it difficult to protect fungal diversity and utilize fungi to improve restoration success. To understand the effects of restoration decisions and environmental variation on fungal communities, we sequenced soil fungal microbiomes from 96 sites across eight experimental Everglades tree islands approximately 15 years after restoration occurred. We found that early restoration decisions can have enduring consequences for fungal communities. Factors experimentally manipulated in 2003–2007 (e.g., type of island core) had significant legacy effects on fungal community composition. Our results also emphasized the role of water regime in fungal diversity, composition, and function. As the relative water level decreased, so did fungal diversity, with an approximately 25% decline in the driest sites. Further, as the water level decreased, the abundance of the plant pathogen–saprotroph guild increased, suggesting that low water may increase plant-pathogen interactions. Our results indicate that early restoration decisions can have long-term consequences for fungal community composition and function and suggest that a drier future in the Everglades could reduce fungal diversity on imperiled tree islands.

scholarly journals A Simple Method for Separating Serum Lipoproteins by Electrophoresis on Cellulose Acetate

1970 ◽  
Vol 16 (5) ◽  
pp. 362-365 ◽  
Author(s):  
Margaret J Fletcher ◽  
Maria H Styliou
Keyword(s):  
Lipid Metabolism ◽  
Cellulose Acetate ◽  
Rapid Method ◽  
Serum Lipoproteins ◽  
Simple Method

Abstract A simple, rapid method is described for separating the serum lipoproteins into clear, discrete, and reproducible bands by electrophoresis on cellulose acetate. Sera of patients with primary or secondary hyperlipidemias show patterns reflecting changes in lipid metabolism. The method would be appropriate for use in screening surveys.

scholarly journals Estimation of Fungal Diversity and Identification of Major Abiotic Drivers Influencing Fungal Richness and Communities in Northern Temperate and Boreal Quebec Forests

Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1096 ◽  
Author(s):  
Laperriere Genevieve ◽  
Chagnon Pierre-Luc ◽  
Giguère-Tremblay Roxanne ◽  
Morneault Amélie ◽  
Bisson Danny ◽  
...  
Keyword(s):  
Soil Ph ◽  
Fungal Community ◽  
Boreal Forests ◽  
Fungal Diversity ◽  
Linear Models ◽  
Species Conservation ◽  
Environmental Filtering ◽  
Operational Taxonomic Unit ◽  
Stand Age ◽  
High Turnover

Fungi play important roles in forest ecosystems and understanding fungal diversity is crucial to address essential questions about species conservation and ecosystems management. Changes in fungal diversity can have severe impacts on ecosystem functionality. Unfortunately, little is known about fungal diversity in northern temperate and boreal forests, and we have yet to understand how abiotic variables shape fungal richness and composition. Our objectives were to make an overview of the fungal richness and the community composition in the region and identify their major abiotic drivers. We sampled 262 stands across the northern temperate and boreal Quebec forest located in the region of Abitibi-Témiscamingue, Mauricie, and Haute-Mauricie. At each site, we characterized fungal composition using Illumina sequencing, as well as several potential abiotic drivers (e.g., humus thickness, soil pH, vegetation cover, etc.). We tested effects of abiotic drivers on species richness using generalized linear models, while difference in fungal composition between stands was analyzed with permutational multivariate analysis of variance and beta-diversity partitioning analyses. Fungi from the order Agaricales, Helotiales, and Russulales were the most frequent and sites from the north of Abitibi-Témiscamingue showed the highest OTUs (Operational Taxonomic Unit) richness. Stand age and moss cover were the best predictors of fungal richness. On the other hand, the strongest drivers of fungal community structure were soil pH, average cumulative precipitation, and stand age, although much of community variance was left unexplained in our models. Overall, our regional metacommunity was characterized by high turnover rate, even when rare OTUs were removed. This may indicate strong environmental filtering by several unmeasured abiotic filters, or stronger than expected dispersal limitations in soil fungal communities. Our results show how difficult it can be to predict fungal community assembly even with high replication and efforts to include several biologically relevant explanatory variables.

scholarly journals Responses of Rhizosphere Fungal Communities to the Sewage Sludge Application into the Soil

2019 ◽  
Vol 7 (11) ◽  
pp. 505 ◽  
Author(s):  
Katarína Ondreičková ◽  
Marcela Gubišová ◽  
Michaela Piliarová ◽  
Miroslav Horník ◽  
Pavel Matušinský ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Fungal Community ◽  
18S Rdna ◽  
Mycorrhizal Fungi ◽  
Fungal Diversity ◽  
Municipal Wastewater ◽  
Environmental Changes ◽  
Community Diversity ◽  
Rdna Sequencing ◽  
Sludge Application

Due to the increasing sewage sludge production in the world and problems with its disposal, an application of sludge to the soil appears to be a suitable solution considering its fertilizer properties and ability to improve the soil physical conditions. On the other hand, the sludge may also contain undesirable and toxic substances. Since soil microorganisms are sensitive to environmental changes, they can be used as indicators of soil quality. In this study, we used sewage sludge (SS) from two municipal wastewater treatment plants (SS-A and SS-B) in the dose of 5 t/ha and 15 t/ha in order to determine possible changes in the fungal community diversity, especially arbuscular mycorrhizal fungi (AMF), in the rhizosphere of Arundo donax L. Rhizosphere samples were collected in summer and autumn for two consecutive years and the fungal diversity was examined using terminal restriction fragment length polymorphism and 18S rDNA sequencing. Fungal alpha diversity was more affected by SS-A than SS-B probably due to the higher heavy metal content. However, based on principal component analysis and ANOSIM, significant changes in overall fungal diversity were not observed. Simultaneously, 18S rDNA sequencing showed that more various fungal taxa were detected in the sample with sewage sludge than in the control. Glomus sp. as a representative of AMF was the most represented. Moreover, Funneliformis in both samples and Rhizophagus in control with Septoglomus in the sludge sample were other representatives of AMF. Our results indicate that the short-term sewage sludge application into the soil does not cause a shift in the fungal community composition.

Simple Method for Concentration of Cerebrospinal Fluid for Protein Electrophoresis

1965 ◽  
Vol 11 (12) ◽  
pp. 1068-1069 ◽  
Author(s):  
Stanley Burrows
Keyword(s):  
Cerebrospinal Fluid ◽  
Rapid Method ◽  
Protein Electrophoresis ◽  
Protein Solutions ◽  
Simple Method ◽  
Dextran Solution

Abstract A rapid method for concentrating protein solutions is described. It utilizes dialysis against a dextran solution under vacuum.

scholarly journals Temporal and Cultivar-Specific Effects on Potato Root- and Rhizosphere Fungal Diversity

2020 ◽  
Author(s):  
Kaire Loit ◽  
Liina Soonvald ◽  
Alar Astover ◽  
Eve Runno-Paurson ◽  
Maarja Öpik ◽  
...  
Keyword(s):  
Community Composition ◽  
Fungal Community ◽  
Fungal Diversity ◽  
High Throughput Sequencing ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Substantial Effect ◽  
Sampling Time ◽  
Fungal Communities ◽  
Fungal Community Composition

The rhizosphere fungal community can play an important role in determining plant growth and health. In this study, using high-throughput sequencing, we investigated the fungal diversity and community composition in the roots and rhizosphere soil of 21 potato (Solanum tuberosum L.) cultivars. The samples were collected at three different sampling points. Furthermore, we assessed the differences in both diversity and composition of pathogen and saprotroph communities. In soil and roots, the fungal richness and relative abundance of pathogens and saprotrophs were mainly affected by sampling time. However, root fungal communities were also significantly affected by cultivar. The most substantial effect of cultivar was on root pathogen diversity. Moreover, the occurrence of most pathogens strongly varied among cultivars. Soil fungal community composition was primarily determined by sampling time; whereas in roots, the primary determinant was cultivar. Our results demonstrate changes in fungal communities over the potato growing season, as well as highlight the importance of potato cultivar on root fungal communities, and emphasise their importance in plant breeding.

scholarly journals Fungal community and functional responses to soil warming are greater than for soil nitrogen enrichment

Elem Sci Anth ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
M. A. Anthony ◽  
M. Knorr ◽  
J. A. M. Moore ◽  
M. Simpson ◽  
S. D. Frey
Keyword(s):  
Global Change ◽  
Soil Carbon ◽  
Carbon Storage ◽  
Fungal Community ◽  
Fungal Diversity ◽  
Hydrolytic Enzyme ◽  
Nitrogen Addition ◽  
Soil Warming ◽  
Soil Carbon Content ◽  
Encoding Genes

Soil fungi are key regulators of forest carbon cycling and their responses to global change have effects that ripple throughout ecosystems. Global changes are expected to push many fungi beyond their environmental niches, but there are relatively few studies involving multiple, simultaneous global change factors. Here, we studied soil fungal diversity, community composition, co-occurrence patterns, and decomposition gene responses to 10 years of soil warming and nitrogen addition, alone and in combination. We specifically examined whether there were fungal community characteristics that could explain changes in soil carbon storage and organic matter chemistry in chronically warmed and fertilized soil. We found that fungal communities in warmed soils are less diverse and shift in composition. Warming also favored hyperdominance by a few mycorrhizal fungal species and lowered manganese peroxidase but increased hydrolytic enzyme encoding gene potentials. Nitrogen addition did not significantly affect fungal community composition but, like warming, did reduce fungal diversity and favored overdominance by a unique set of mycorrhizal taxa. Warming alone and in combination with nitrogen addition also reduced negative but increased positive fungal co-occurrence probabilities, promoting species coexistence. Negative fungal co-occurrence was positively correlated to soil carbon content, while the proportion of fungal hydrolytic enzyme encoding genes was negatively correlated with soil carbon content. This may reflect fungal life history trade-offs between competition (e.g., reduced negative co-occurrence) and resource acquisition (e.g., higher abundance of hydrolytic enzyme encoding genes) with implications for carbon storage.

scholarly journals Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration

2020 ◽  
Vol 5 (04) ◽  
pp. 54-59
Author(s):  
Nazia Begum ◽  
Kandavalli Manipriya ◽  
Rahathunnisa Begum ◽  
Veeresh B
Keyword(s):  
Estrous Cycle ◽  
Rapid Method ◽  
Crystal Violet ◽  
Vaginal Smear ◽  
Simple Method ◽  
Ovarian Morphology ◽  
Female Wistar Rats ◽  
Vaginal Cytology ◽  
Histomorphological Changes ◽  
Hormonal Levels

Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology and reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination in rats using crystal violet. With this technique, the identification of stages can be done even on the next day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the estrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works related to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the estrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet and to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.

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