scholarly journals Charge injection based electrical stimulation on polypyrrole planar electrodes to regulate cellular osteogenic differentiation

RSC Advances ◽  
2018 ◽  
Vol 8 (33) ◽  
pp. 18470-18479 ◽  
Author(s):  
Zongguang Liu ◽  
Lingqing Dong ◽  
Kui Cheng ◽  
Zhongkuan Luo ◽  
Wenjian Weng
Keyword(s):  
Electrical Stimulation ◽  
Osteogenic Differentiation ◽  
Applied Voltage ◽  
Charge Injection ◽  
Planar Electrodes

This study reveals that the Qinj on electrodes is a more significant factor than applied voltage for electrical stimulation to regulate cellular osteogenic differentiation, and the charge injection capacity can be tuned by thickness of Ppy.

THEORETICAL ANALYSIS AND EXPERIMENT OF A NOVEL DEP CHIP WITH 3-D SILICON ELECTRODES

2005 ◽  
Vol 15 (02) ◽  
pp. 231-236 ◽  
Author(s):  
LIMING YU ◽  
FRANCIS E. H. TAY ◽  
GUOLIN XU ◽  
CIPRIAN ILIESCU ◽  
MARIOARA AVRAM
Keyword(s):  
Theoretical Analysis ◽  
Applied Voltage ◽  
Microfluidic Channel ◽  
The Other ◽  
Yeast Cells ◽  
Applied Potential ◽  
Dielectrophoretic Force ◽  
Joule Effect ◽  
Doped Silicon ◽  
Planar Electrodes

This paper presents a novel dielectrophoresis (DEP) device where the DEP electrodes define the channel walls. This is achieved by fabricating microfluidic channel walls from highly doped silicon so that they can also function as DEP electrodes. Compared with planar electrodes, this device increases the exhibited dielectrophoretic force on the particle, therefore decreases the applied potential and reduces the heating of the solution. A DEP device with triangle electrodes has been designed and fabricated. Compared with the other two configurations, semi-circular and square, triangle electrode presents an increased force, which can decrease the applied voltage and reduce the Joule effect. Yeast cells have been used to for testing the performance of the device.

scholarly journals The Bioactive Polypyrrole/Polydopamine Nanowire Coating with Enhanced Osteogenic Differentiation Ability with Electrical Stimulation

Coatings ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1189
Author(s):  
Yuan He ◽  
Lingfeng Dai ◽  
Xiuming Zhang ◽  
Yanan Sun ◽  
Wei Shi ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Tissue Repair ◽  
Step Method ◽  
Functional Coating ◽  
Tissue Repair And Regeneration ◽  
Cell Affinity ◽  
Excellent Adhesion ◽  
Cells Cultured

Polypyrrole (PPy) is a promising conducting polymer in bone regeneration; however, due to the biological inertia of the PPy surface, it has poor cell affinity and bioactivity. Based on the excellent adhesion capacity, biocompatibility, and bioactivity of polydopamine (PDA), the PDA is used as a functional coating in tissue repair and regeneration. Herein, we used a two-step method to construct a functional conductive coating of polypyrrole/polydopamine (PPy/PDA) nanocomposite for bone regeneration. PPy nanowires (NWs) are used as the morphologic support layer, and a layer of highly bioactive PDA is introduced on the surface of PPy NWs by solution oxidation. By controlling the depositing time of PDA within 5 h, the damage of nano morphology and conductivity of the PPy NWs caused by the coverage of PDA deposition layer can be effectively avoided, and the thin PDA layer also significantly improve the hydrophilicity, adhesion, and biological activity of PPy NWs coating. The PPy/PDA NWs coating performs better biocombaitibility and bioactivity than pure PPy NWs and PDA, and has benefits for the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells cultured on the surface. In addition, PPy/PDA NWs can significantly promote the osteogenesis of MC3T3-E1 in combination with micro galvanostatic electrical stimulation (ES).

scholarly journals Corrosion Study of Implanted Tin Electrodes Using Excessive Electrical Stimulation in Minipigs

Metals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 389 ◽  
Author(s):  
Suzan Meijs ◽  
Kristian Rechendorff ◽  
Søren Sørensen ◽  
Nico Rijkhoff
Keyword(s):  
Electrical Stimulation ◽  
Tissue Damage ◽  
Charge Injection ◽  
The Other ◽  
Electrochemical Tests ◽  
Implanted Electrodes ◽  
Electrode Degradation ◽  
Lower Charge

(1) Background: Titanium nitride (TiN) electrodes have been used for implantable stimulation and sensing electrodes for decades. Nevertheless, there still is a discrepancy between the in vitro and in vivo determined safe charge injection limits. This study investigated the consequences of pulsing implanted electrodes beyond the in vivo safe charge injection limits. (2) Methods: The electrodes were implanted for a month and then pulsed at 20 mA and 50 mA and 200 Hz and 400 Hz. Afterwards, the electrodes were investigated using electrochemical and analytical methods to evaluate whether electrode degradation had occurred. (3) Results: Electrochemical tests showed that electrodes that pulsed at 20 mA and 200 Hz (lowest electrical dose) had a significantly lower charge injection capacity and higher impedance than the other used and unused electrodes. (4) Conclusions: The electrodes pulsed at the lowest electrical dose, for which no tissue damage was found, appeared to have degraded. Electrodes pulsed at higher electrical doses for which tissue damage did occur, on the other hand, show no significant degradation in electrochemical tests compared to unused implanted and not implanted electrodes. It is thus clear that the tissue surrounding the electrode has an influence on the charge injection properties of the electrodes and vice versa.

scholarly journals Pretreating mesenchymal stem cells with electrical stimulation causes sustained long-lasting pro-osteogenic effects

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4959 ◽  
Author(s):  
Maria Eischen-Loges ◽  
Karla M.C. Oliveira ◽  
Mit B. Bhavsar ◽  
John H. Barker ◽  
Liudmila Leppik
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Therapeutic Potential ◽  
Osteogenic Marker ◽  
Osteogenic Activity ◽  
Osteogenic Effect

Background Electrical stimulation (ES) has a long history of successful use in the clinical treatment of refractory, non-healing bone fractures and has recently been proposed as an adjunct to bone tissue-engineering treatments to optimize their therapeutic potential. This idea emerged from ES’s demonstrated positive effects on stem cell migration, proliferation, differentiation and adherence to scaffolds, all cell behaviors recognized to be advantageous in Bone Tissue Engineering (BTE). In previous in vitro experiments we demonstrated that direct current ES, administered daily, accelerates Mesenchymal Stem Cell (MSC) osteogenic differentiation. In the present study, we sought to define the optimal ES regimen for maximizing this pro-osteogenic effect. Methods Rat bone marrow-derived MSC were exposed to 100 mV/mm, 1 hr/day for three, seven, and 14 days, then osteogenic differentiation was assessed at Day 14 of culture by measuring collagen production, calcium deposition, alkaline phosphatase activity and osteogenic marker gene expression. Results We found that exposing MSC to ES for three days had minimal effect, while seven and 14 days resulted in increased osteogenic differentiation, as indicated by significant increases in collagen and calcium deposits, and expression of osteogenic marker genes Col1a1, Osteopontin, Osterix and Calmodulin. We also found that cells treated with ES for seven days, maintained this pro-osteogenic activity long (for at least seven days) after discontinuing ES exposure. Discussion This study showed that while three days of ES is insufficient to solicit pro-osteogenic effects, seven and 14 days significantly increases osteogenic differentiation. Importantly, we found that cells treated with ES for only seven days, maintained this pro-osteogenic activity long after discontinuing ES exposure. This sustained positive osteogenic effect is likely due to the enhanced expression of RunX2 and Calmodulin we observed. This prolonged positive osteogenic effect, long after discontinuing ES treatment, if incorporated into BTE treatment protocols, could potentially improve outcomes and in doing so help BTE achieve its full therapeutic potential.

Roles of electrical stimulation in promoting osteogenic differentiation of BMSCs on conductive fibers

2019 ◽  
Vol 107 (7) ◽  
pp. 1443-1454 ◽  
Author(s):  
Wei Jing ◽  
Yiqian Huang ◽  
Pengfei Wei ◽  
Qing Cai ◽  
Xiaoping Yang ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Osteogenic Differentiation ◽  
Conductive Fibers
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