The effects of substrate‐mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization

2018 ◽  
Vol 107 (5) ◽  
pp. 1607-1619 ◽  
Author(s):  
Wei‐Wen Hu ◽  
Tun‐Chi Chen ◽  
Chia‐Wen Tsao ◽  
Yu‐Che Cheng
Keyword(s):  
Electrical Stimulation ◽  
Osteogenic Differentiation

scholarly journals The Bioactive Polypyrrole/Polydopamine Nanowire Coating with Enhanced Osteogenic Differentiation Ability with Electrical Stimulation

Coatings ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1189
Author(s):  
Yuan He ◽  
Lingfeng Dai ◽  
Xiuming Zhang ◽  
Yanan Sun ◽  
Wei Shi ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Tissue Repair ◽  
Step Method ◽  
Functional Coating ◽  
Tissue Repair And Regeneration ◽  
Cell Affinity ◽  
Excellent Adhesion ◽  
Cells Cultured

Polypyrrole (PPy) is a promising conducting polymer in bone regeneration; however, due to the biological inertia of the PPy surface, it has poor cell affinity and bioactivity. Based on the excellent adhesion capacity, biocompatibility, and bioactivity of polydopamine (PDA), the PDA is used as a functional coating in tissue repair and regeneration. Herein, we used a two-step method to construct a functional conductive coating of polypyrrole/polydopamine (PPy/PDA) nanocomposite for bone regeneration. PPy nanowires (NWs) are used as the morphologic support layer, and a layer of highly bioactive PDA is introduced on the surface of PPy NWs by solution oxidation. By controlling the depositing time of PDA within 5 h, the damage of nano morphology and conductivity of the PPy NWs caused by the coverage of PDA deposition layer can be effectively avoided, and the thin PDA layer also significantly improve the hydrophilicity, adhesion, and biological activity of PPy NWs coating. The PPy/PDA NWs coating performs better biocombaitibility and bioactivity than pure PPy NWs and PDA, and has benefits for the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells cultured on the surface. In addition, PPy/PDA NWs can significantly promote the osteogenesis of MC3T3-E1 in combination with micro galvanostatic electrical stimulation (ES).

scholarly journals Pretreating mesenchymal stem cells with electrical stimulation causes sustained long-lasting pro-osteogenic effects

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4959 ◽  
Author(s):  
Maria Eischen-Loges ◽  
Karla M.C. Oliveira ◽  
Mit B. Bhavsar ◽  
John H. Barker ◽  
Liudmila Leppik
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Therapeutic Potential ◽  
Osteogenic Marker ◽  
Osteogenic Activity ◽  
Osteogenic Effect

Background Electrical stimulation (ES) has a long history of successful use in the clinical treatment of refractory, non-healing bone fractures and has recently been proposed as an adjunct to bone tissue-engineering treatments to optimize their therapeutic potential. This idea emerged from ES’s demonstrated positive effects on stem cell migration, proliferation, differentiation and adherence to scaffolds, all cell behaviors recognized to be advantageous in Bone Tissue Engineering (BTE). In previous in vitro experiments we demonstrated that direct current ES, administered daily, accelerates Mesenchymal Stem Cell (MSC) osteogenic differentiation. In the present study, we sought to define the optimal ES regimen for maximizing this pro-osteogenic effect. Methods Rat bone marrow-derived MSC were exposed to 100 mV/mm, 1 hr/day for three, seven, and 14 days, then osteogenic differentiation was assessed at Day 14 of culture by measuring collagen production, calcium deposition, alkaline phosphatase activity and osteogenic marker gene expression. Results We found that exposing MSC to ES for three days had minimal effect, while seven and 14 days resulted in increased osteogenic differentiation, as indicated by significant increases in collagen and calcium deposits, and expression of osteogenic marker genes Col1a1, Osteopontin, Osterix and Calmodulin. We also found that cells treated with ES for seven days, maintained this pro-osteogenic activity long (for at least seven days) after discontinuing ES exposure. Discussion This study showed that while three days of ES is insufficient to solicit pro-osteogenic effects, seven and 14 days significantly increases osteogenic differentiation. Importantly, we found that cells treated with ES for only seven days, maintained this pro-osteogenic activity long after discontinuing ES exposure. This sustained positive osteogenic effect is likely due to the enhanced expression of RunX2 and Calmodulin we observed. This prolonged positive osteogenic effect, long after discontinuing ES treatment, if incorporated into BTE treatment protocols, could potentially improve outcomes and in doing so help BTE achieve its full therapeutic potential.

Roles of electrical stimulation in promoting osteogenic differentiation of BMSCs on conductive fibers

2019 ◽  
Vol 107 (7) ◽  
pp. 1443-1454 ◽  
Author(s):  
Wei Jing ◽  
Yiqian Huang ◽  
Pengfei Wei ◽  
Qing Cai ◽  
Xiaoping Yang ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Osteogenic Differentiation ◽  
Conductive Fibers

A microelectrode array chip for osteogenic differentiation of mesenchymal stem cells under electrical stimulation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 373-383 ◽  
Author(s):  
Tianyang Zheng ◽  
Zhizhong Zhang ◽  
Rong Zhu ◽  
Dong Sun
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Electric Field ◽  
Osteogenic Differentiation ◽  
Microelectrode Array ◽  
Uniform Electric Field

A microelectrode array chip is able to improve the osteogenic differentiation of mesenchymal stem cells by generating a non-uniform electric field.

An electrically and magnetically responsive nanocomposite of GdPO4·H2O/P3HT/PLGA with electrical stimulation for synergistically enhancing the proliferation and differentiation of pre-osteoblasts

2019 ◽  
Vol 43 (44) ◽  
pp. 17315-17326 ◽  
Author(s):  
Huanhuan Yan ◽  
Linlong Li ◽  
Yu Wang ◽  
Jing Huang ◽  
Zongliang Wang ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Osteogenic Differentiation ◽  
Proliferation And Differentiation

The electric–magnetic responsive nanocomposite GdPO4·H2O/P3HT/PLGA could enhance MRI signals, and synergistically accelerate proliferation and osteogenic differentiation upon electrical stimulation.

Conductive stretchable shape memory elastomers combining with electrical stimulation for synergistic osteogenic differentiation

2020 ◽  
Vol 90 ◽  
pp. 106672
Author(s):  
Huanhuan Yan ◽  
Linlong Li ◽  
Xincui Shi ◽  
Jui-Ming Yeh ◽  
Yen Wei ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Shape Memory ◽  
Osteogenic Differentiation
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