Aminoluciferin 4-hydroxyphenyl amide enables bioluminescence detection of endogenous tyrosinase

2018 ◽  
Vol 16 (47) ◽  
pp. 9197-9203 ◽  
Author(s):  
Chunchao Tang ◽  
Lei Jin ◽  
Yuxing Lin ◽  
Jing Su ◽  
Yingai Sun ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Living Cells ◽  
Tyrosinase Activity ◽  
Cell Permeability ◽  
Sensitivity And Selectivity ◽  
Good Cell

We report a new BL probe,TyrBP-3, which not only exhibits high sensitivity and selectivity for imaging tyrosinase in vitro, and good cell-permeability for detecting tyrosinase in living cells, but can also visualize the level of tyrosinase activity in tumors of living animals.

scholarly journals Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo

Cancers ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 470 ◽  
Author(s):  
Surendra Punganuru ◽  
Hanumantha Madala ◽  
Viswanath Arutla ◽  
Kalkunte Srivenugopal
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Stokes Shift ◽  
High Sensitivity ◽  
Cell Permeability ◽  
Quinone Oxidoreductase ◽  
Brain Tumor Cells ◽  
Sensitivity And Selectivity

Human NAD(P)H quinone oxidoreductase-1 (hNQO1) is an important cancer-related biomarker, which shows significant overexpression in malignant cells. Developing an effective method for detecting NQO1 activity with high sensitivity and selectivity in tumors holds a great potential for cancer diagnosis, treatment, and management. In the present study, we report a new dicyanoisophorone (DCP) based fluorescent probe (NQ-DCP) capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model. NQ-DCP was prepared by conjugating dicyanoisophorone fluoroprobe with hNQO1 activatable quinone propionic acid (QPA), which remain non-fluorescent until activation by tumor-specific hNQO1. NQ-DCP featured a large Stokes shift (145 nm), excellent biocompatibility, cell permeability, and selectivity towards hNQO1 allowed to differentiate cancer cells from healthy cells. We have successfully employed NQ-DCP to monitor non-invasive endogenous hNQO1 activity in brain tumor cells in vitro and in xenografted tumors developed in nude mice.

scholarly journals Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2577 ◽  
Author(s):  
Yuanying Liang ◽  
Ting Guo ◽  
Lei Zhou ◽  
Andreas Offenhäusser ◽  
Dirk Mayer
Keyword(s):  
Detection Limit ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Label Free ◽  
Dopamine Detection ◽  
Electrochemical Transistor ◽  
Sensitivity And Selectivity

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.

Pyrene appended thymine derivative for selective turn-on fluorescence sensing of uric acid in live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66774-66778 ◽  
Author(s):  
Prithidipa Sahoo ◽  
Himadri Sekhar Sarkar ◽  
Sujoy Das ◽  
Kalipada Maiti ◽  
Md Raihan Uddin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
First Time

A new ‘turn-ON’ fluorescent probe, pyrene appended thymine acetamide (PTA), with high sensitivity and selectivity for the detection of uric acid (UA) was developed and first time imaging of uric acid in living cells in water was achieved.

A smart sensor for rapid detection of lethal hydrazine in human blood and drinking water

2019 ◽  
Vol 43 (8) ◽  
pp. 3303-3308 ◽  
Author(s):  
Sima Paul ◽  
Rajesh Nandi ◽  
Kakali Ghoshal ◽  
Maitree Bhattacharyya ◽  
Dilip K. Maiti
Keyword(s):  
Drinking Water ◽  
Health Hazard ◽  
Industrial Effluent ◽  
Living Cells ◽  
Cell Permeability ◽  
Smart Sensor ◽  
Naked Eye ◽  
Good Cell ◽  
Low Cytotoxicity ◽  
Naked Eye Detection

A newly designed and synthesized probe showed good cell permeability, low cytotoxicity, fast fluorogenic recognition, and “naked-eye” detection of a lethal health hazard, hydrazine, even at concentrations significantly below the TLV levels present in living cells, drinking water and industrial effluent.

scholarly journals Nanomaterials-Based Electrochemical Sensors for In Vitro and In Vivo Analyses of Neurotransmitters

2018 ◽  
Vol 8 (9) ◽  
pp. 1504 ◽  
Author(s):  
Sharmila Durairaj ◽  
Boopathi Sidhureddy ◽  
Joseph Cirone ◽  
Aicheng Chen
Keyword(s):  
Electrochemical Sensors ◽  
Point Of Care ◽  
Electrochemical Techniques ◽  
High Sensitivity ◽  
Electrochemical Analysis ◽  
Detection Techniques ◽  
Efficient Detection ◽  
Sensitivity And Selectivity

Neurotransmitters are molecules that transfer chemical signals between neurons to convey messages for any action conducted by the nervous system. All neurotransmitters are medically important; the detection and analysis of these molecules play vital roles in the diagnosis and treatment of diseases. Among analytical strategies, electrochemical techniques have been identified as simple, inexpensive, and less time-consuming processes. Electrochemical analysis is based on the redox behaviors of neurotransmitters, as well as their metabolites. A variety of electrochemical techniques are available for the detection of biomolecules. However, the development of a sensing platform with high sensitivity and selectivity is challenging, and it has been found to be a bottleneck step in the analysis of neurotransmitters. Nanomaterials-based sensor platforms are fascinating for researchers because of their ability to perform the electrochemical analysis of neurotransmitters due to their improved detection efficacy, and they have been widely reported on for their sensitive detection of epinephrine, dopamine, serotonin, glutamate, acetylcholine, nitric oxide, and purines. The advancement of electroanalytical technologies and the innovation of functional nanomaterials have been assisting greatly in in vivo and in vitro analyses of neurotransmitters, especially for point-of-care clinical applications. In this review, firstly, we focus on the most commonly employed electrochemical analysis techniques, in conjunction with their working principles and abilities for the detection of neurotransmitters. Subsequently, we concentrate on the fabrication and development of nanomaterials-based electrochemical sensors and their advantages over other detection techniques. Finally, we address the challenges and the future outlook in the development of electrochemical sensors for the efficient detection of neurotransmitters.

scholarly journals An ESIPT based naphthalimide chemosensor for visualizing endogenous ONOO− in living cells

RSC Advances ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 1826-1832 ◽  
Author(s):  
Yunshuang Fu ◽  
Hailiang Nie ◽  
Rubo Zhang ◽  
Fangyun Xin ◽  
Yong Tian ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Living Cells ◽  
Sensitivity And Selectivity

An ESIPT based naphthalimide chemosensor with high sensitivity and selectivity for visualizing endogenous ONOO− in living cells was developed.

scholarly journals Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 517 ◽  
Author(s):  
Nicolae-Bogdan Mincu ◽  
Veronica Lazar ◽  
Dana Stan ◽  
Carmen Marinela Mihailescu ◽  
Rodica Iosub ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Diagnosis ◽  
Diagnostic Purpose ◽  
Pathogenic Microorganisms ◽  
Screen Printed Electrodes ◽  
High Incidence ◽  
In Vitro Diagnostic ◽  
Sensitivity And Selectivity ◽  
Screen Printed

Due to rapidly spreading infectious diseases and the high incidence of other diseases such as cancer or metabolic syndrome, there is a continuous need for the development of rapid and accurate diagnosis methods. Screen-printed electrodes-based biosensors have been reported to offer reliable results, with high sensitivity and selectivity and, in some cases, low detection limits. There are a series of materials (carbon, gold, platinum, etc.) used for the manufacturing of working electrodes. Each version comes with advantages, as well as challenges for their functionalization. Thus, the aim is to review the most promising biosensors developed using screen-printed electrodes for the detection/quantification of proteins, biomarkers, or pathogenic microorganisms.

A fluorescence-enhanced probe for rapid detection of formaldehyde and its application for cell imaging

2017 ◽  
Vol 9 (37) ◽  
pp. 5472-5477 ◽  
Author(s):  
Feng Wu ◽  
Yue Zhang ◽  
Lei Huang ◽  
Dan Xu ◽  
Hongying Wang
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Cell Imaging ◽  
High Sensitivity ◽  
Living Cells ◽  
Sensitivity And Selectivity

An anthracene carboxyimide-based fluorescent probe was developed for rapid detection of formaldehyde in living cells with high sensitivity and selectivity.

Purification of Classical Swine Fever Virus E2 Subunit Vaccines basing on High Affinity Peptide Ligand.

2020 ◽  
Vol 27 ◽  
Author(s):  
Fangyu Wang ◽  
Qiuying Yu ◽  
Man Hu ◽  
Guangxu Xing ◽  
Dong Zhao ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Rapid Development ◽  
Classical Swine Fever ◽  
High Sensitivity ◽  
Fever Virus ◽  
Affinity Constant ◽  
Peptide Ligand ◽  
Binding Interaction ◽  
Sensitivity And Selectivity

Background: The purification of expressed proteins is the most critical part of subunit-vaccine production. Protein-purification methods such as affinity chromatography and ion exchange still have the shortcomings of being time consuming and complicated. With the rapid development of computational molecular-simulation technology, structure-based peptide-ligand design has become feasible. Objection: We aimed to apply molecular docking for a peptide ligand designed for classical swine fever virus (CSFV) E2 purification. Methods: Computational-derived peptides were synthesized, and the in vitro binding interaction with E2 was investigated. The effects of purification on E2 were also evaluated. Results: The best peptide recognizing E2 was P6, which had a sequence of KKFYWRYWEH. Based on kinetic surface plasmon resonance (SPR) analysis, the apparent affinity constant of P6 was found to be 148 nM. Importantly, P6 showed suitable binding affinity and specificity for E2 purification from transgenic rice seeds. Evaluation of immune antibodies in mice showed that the antibody-blocking rate on day 42 after inoculation reached 86.18% and 90.68%. Conclusion: The computational-designed peptide in this study has high sensitivity and selectivity and is thus useful for the purification of CSFV E2. The novel method of design provided a broad platform and powerful tool for protein-peptide screening, as well as new insights into CSFV vaccine design.

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