Bacterial biofilm destruction by size/surface charge-adaptive micelles

Nanoscale ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 1410-1422 ◽  
Author(s):  
Maohua Chen ◽  
Jiaojun Wei ◽  
Songzhi Xie ◽  
Xinyan Tao ◽  
Zhanlin Zhang ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Surface Charge ◽  
Pathogenic Bacteria ◽  
Bacterial Biofilm ◽  
Biofilm Destruction

Biofilms formed by pathogenic bacteria are one of the most important reasons for multidrug resistance.

Antibiotics Application Strategies to Control Biofilm Formation in Pathogenic Bacteria

2020 ◽  
Vol 21 (4) ◽  
pp. 270-286 ◽  
Author(s):  
Fazlurrahman Khan ◽  
Dung T.N. Pham ◽  
Sandra F. Oloketuyi ◽  
Young-Mog Kim
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Extracellular Polymeric Substances ◽  
Quorum Quenching ◽  
Resistance Mechanisms ◽  
Bacterial Biofilm ◽  
Bacterial Cells ◽  
High Concentration ◽  
Biofilm Inhibition ◽  
Abiotic Surfaces

Background: The establishment of a biofilm by most pathogenic bacteria has been known as one of the resistance mechanisms against antibiotics. A biofilm is a structural component where the bacterial community adheres to the biotic or abiotic surfaces by the help of Extracellular Polymeric Substances (EPS) produced by bacterial cells. The biofilm matrix possesses the ability to resist several adverse environmental factors, including the effect of antibiotics. Therefore, the resistance of bacterial biofilm-forming cells could be increased up to 1000 times than the planktonic cells, hence requiring a significantly high concentration of antibiotics for treatment. Methods: Up to the present, several methodologies employing antibiotics as an anti-biofilm, antivirulence or quorum quenching agent have been developed for biofilm inhibition and eradication of a pre-formed mature biofilm. Results: Among the anti-biofilm strategies being tested, the sub-minimal inhibitory concentration of several antibiotics either alone or in combination has been shown to inhibit biofilm formation and down-regulate the production of virulence factors. The combinatorial strategies include (1) combination of multiple antibiotics, (2) combination of antibiotics with non-antibiotic agents and (3) loading of antibiotics onto a carrier. Conclusion: The present review paper describes the role of several antibiotics as biofilm inhibitors and also the alternative strategies adopted for applications in eradicating and inhibiting the formation of biofilm by pathogenic bacteria.

scholarly journals Surface charge modulation of rifampicin-loaded PLA nanoparticles to improve antibiotic delivery in Staphylococcus aureus biofilms

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
David Da Costa ◽  
Chloé Exbrayat-Héritier ◽  
Basile Rambaud ◽  
Simon Megy ◽  
Raphaël Terreux ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Surface Charge ◽  
Treatment Efficacy ◽  
Particle Migration ◽  
Bacterial Biofilm ◽  
Poly Lactic Acid ◽  
Retention Capacity ◽  
The Impact ◽  
Positively Charged ◽  
Antibiotic Delivery

Abstract Background After the golden age of antibiotic discovery, bacterial infections still represent a major challenge for public health worldwide. The biofilm mode of growth is mostly responsible for chronic infections that current therapeutics fail to cure and it is well-established that novel strategies must be investigated. Particulate drug delivery systems are considered as a promising strategy to face issues related to antibiotic treatments in a biofilm context. Particularly, poly-lactic acid (PLA) nanoparticles present a great interest due to their ability to migrate into biofilms thanks to their submicronic size. However, questions still remain unresolved about their mode of action in biofilms depending on their surface properties. In the current study, we have investigated the impact of their surface charge, firstly on their behavior within a bacterial biofilm, and secondly on the antibiotic delivery and the treatment efficacy. Results Rifampicin-loaded PLA nanoparticles were synthetized by nanoprecipitation and characterized. A high and superficial loading of rifampicin, confirmed by an in silico simulation, enabled to deliver effective antibiotic doses with a two-phase release, appropriate for biofilm-associated treatments. These nanoparticles were functionalized with poly-l-lysine, a cationic peptide, by surface coating inducing charge reversal without altering the other physicochemical properties of these particles. Positively charged nanoparticles were able to interact stronger than negative ones with Staphylococcus aureus, under planktonic and biofilm modes of growth, leading to a slowed particle migration in the biofilm thickness and to an improved retention of these cationic particles in biofilms. While rifampicin was totally ineffective in biofilms after washing, the increased retention capacity of poly-l-lysine-coated rifampicin-loaded PLA nanoparticles has been associated with a better antibiotic efficacy than uncoated negatively charged ones. Conclusions Correlating the carrier retention capacity in biofilms with the treatment efficacy, positively charged rifampicin-loaded PLA nanoparticles are therefore proposed as an adapted and promising approach to improve antibiotic delivery in S. aureus biofilms.

scholarly journals Antimicrobial Resistance Profiles of Bacteria Isolated from the Nasal Cavity of Camels in Samburu, Nakuru, and Isiolo Counties of Kenya

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
J. M. Mutua ◽  
C. G. Gitao ◽  
L. C. Bebora ◽  
F. K. Mutua
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Nasal Cavity ◽  
Resistance Genes ◽  
Streptococcus Agalactiae ◽  
Pathogenic Bacteria ◽  
Bacterial Isolates ◽  
Human Pathogens ◽  
Normal Flora

This study was designed to determine antimicrobial resistance profiles of bacteria isolated from the nasal cavity of healthy camels. A total of 255 nasal samples (swabs) were collected in Isiolo, Samburu, and Nakuru counties, Kenya, from which 404 bacterial isolates belonging to various genera and species were recovered. The bacterial isolates included Bacillus (39.60%), coagulase-negative Staphylococcus (29.95%), Streptococcus species other than Streptococcus agalactiae (25.74%), coagulase-positive Staphylococcus (3.96%), and Streptococcus agalactiae (0.74%). Isolates were most susceptible to Gentamicin (95.8%), followed by Tetracycline (90.5%), Kanamycin and Chloramphenicol (each at 85.3%), Sulphamethoxazole (84.2%), Co-trimoxazole (82.1%), Ampicillin (78.9%), and finally Streptomycin (76.8%). This translated to low resistance levels. Multidrug resistance was also reported in 30.5% of the isolates tested. Even though the antibiotic resistance demonstrated in this study is low, the observation is significant, since the few resistant normal flora could be harboring resistance genes which can be transferred to pathogenic bacteria within the animal, to other animals’ bacteria and, most seriously, to human pathogens.

scholarly journals Ankyrin-Like Protein AnkB Interacts with CatB, Affects Catalase Activity, and Enhances Resistance ofXanthomonas oryzaepv. oryzae andXanthomonas oryzaepv. oryzicola to Phenazine-1-Carboxylic Acid

2017 ◽  
Vol 84 (4) ◽  
Author(s):  
Xiayan Pan ◽  
Shu Xu ◽  
Jian Wu ◽  
Yabing Duan ◽  
Zhitian Zheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carboxylic Acid ◽  
Catalase Activity ◽  
Pathogenic Bacteria ◽  
Adaptor Protein ◽  
Bacterial Biofilm ◽  
Xanthomonas Oryzae ◽  
Content Type ◽  
Plant Pathogenic Bacteria ◽  
Ankyrin Protein

ABSTRACTXanthomonas oryzaepv. oryzae, which causes rice bacterial leaf blight, andXanthomonas oryzaepv. oryzicola, which causes rice bacterial leaf streak, are important plant-pathogenic bacteria. A member of the adaptor protein family, ankyrin protein, has been investigated largely in humans but rarely in plant-pathogenic bacteria. In this study, a novel ankyrin-like protein, AnkB, was identified inX. oryzaepv. oryzae andX. oryzaepv. oryzicola. The expression ofankBwas significantly upregulated when these bacteria were treated with phenazine-1-carboxylic acid (PCA).ankBis located 58 bp downstream of the genecatB(which encodes a catalase) in both bacteria, and the gene expression ofcatBand catalase activity were reduced followingankBdeletion inX. oryzaepv. oryzae andX. oryzaepv. oryzicola. Furthermore, we demonstrated that AnkB directly interacts with CatB by glutathioneS-transferase (GST) pulldown assays. Deletion ofankBincreased the sensitivity ofX. oryzaepv. oryzae andX. oryzaepv. oryzicola to H2O2and PCA, decreased bacterial biofilm formation, swimming ability, and exopolysaccharide (EPS) production, and also reduced virulence on rice. Together our results indicate that the ankyrin-like protein AnkB has important and conserved roles in antioxidant systems and pathogenicity inX. oryzaepv. oryzae andX. oryzaepv. oryzicola.IMPORTANCEThis study demonstrates that the ankyrin protein AnkB directly interacts with catalase CatB inXanthomonas oryzaepv. oryzae andXanthomonas oryzaepv. oryzicola. Ankyrin protein AnkB can affect the gene expression ofcatB, catalase activity, and sensitivity to H2O2. InXanthomonasspp., the locations of genesankBandcatBand the amino acid sequence of AnkB are highly conserved. It is suggested that in prokaryotes, AnkB plays a conserved role in the defense against oxidative stress.

scholarly journals pH-triggered surface charge-switchable polymer micelles for the co-delivery of paclitaxel/disulfiram and overcoming multidrug resistance in cancer

2017 ◽  
Vol Volume 12 ◽  
pp. 8631-8647 ◽  
Author(s):  
Qiang Huo ◽  
Jianhua Zhu ◽  
Yimin Niu ◽  
Huihui Shi ◽  
Yaxiang Gong ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Surface Charge ◽  
Polymer Micelles

scholarly journals Infection in central venous catheter led by parenteral nutrition of tumor patients

2018 ◽  
Vol 6 (4) ◽  
pp. 103-107
Author(s):  
Dan Wang
Keyword(s):  
Central Venous Catheter ◽  
Parenteral Nutrition ◽  
Pathogenic Bacteria ◽  
Venous Catheter ◽  
Bacterial Biofilm ◽  
High Fat ◽  
Central Venous ◽  
Tumor Patients ◽  
Repeated Infections ◽  
And Diffusion

Abstract With the wide application of parenteral nutrition (PN) and central venous catheter (CVC) in tumor patients, the relative infection led by CVC has become a common and troublesome hospital infection in clinics. During infusion, PN fluid forms a high-sugar and high-fat microenvironment around CVC, facilitating the adhesion, growth, and diffusion of pathogenic bacteria. This condition forms a bacterial biofilm (BF) consisting of one or several kinds of pathogenic bacteria. Upon formation of the film, pathogens in the BF can resist antibacterial drugs and immune cells, causing repeated infections of bacteria or fungi and endangering the lives of patients. In this article, we summarize the applications of PN, characteristics of CVC infection in tumor patients, mixed BF, and related research methods to provide reference for studies of mixed BF infection of CVC.

The impact of the viral-bacterial consortium on occurrence and development of chronic periodontitis

2020 ◽  
Vol 25 (2) ◽  
pp. 84-89
Author(s):  
V. N. Tsarev ◽  
E. A. Yagodina ◽  
T. V. Tsareva ◽  
E. N. Nikolaeva
Keyword(s):  
Pathogenic Bacteria ◽  
Periodontal Diseases ◽  
Bacterial Consortium ◽  
Progressive Increase ◽  
Bacterial Biofilm ◽  
Herpes Simplex Viruses ◽  
Periodontal Tissues ◽  
Periodontal Inflammation ◽  
Immunological Mechanisms ◽  
The Impact

Relevance. The current theory of specific bacterial biofilm fails explain why a part of patients experiences inflammatory periodontal diseases while the absence of detected specific types of “red complex” bacteria.Purpose. To clarify the microbiological and immunological mechanisms of the influence of the viral and bacterial consortium in the etiology and pathogenesis of inflammatory periodontal diseases.Materials and methods. Articles survey with elements of metanalisis. Literature review based on discussion of research results on the topic of 48 sources including 33 foreign ones.Results. The review provides evidences of the possible participation of viruses of the Herpesviridae family in the development of chronic generalized periodontitis. Evidences for the role of herpes simplex viruses of type 1.2, Epstein-Barr virus, and cytomegalovirus in the development of periodontal inflammation are analyzed. It is proven that all herpesviruses induce the release of proinflammatory cytokines that activate osteoclasts and matrix metalloproteinases, as well as violate antibacterial immune mechanisms. In turn that leads to a progressive increase of periodontal pathogenic bacteria in both the biofilm and periodontal tissues.Conclusion. It is made a conclusion that an active herpetic infection can initiate damage to periodontal tissus and participate in the development of relapses of the disease.

ANTIBIOFILM ACTIVITY OF SELECTED PLANT SPECIES

2021 ◽  
Author(s):  
Jelena Terzić ◽  
◽  
Marina Stanković ◽  
Olgica Stefanović
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Ethanol Extract ◽  
Acetone Extract ◽  
Bacterial Biofilm ◽  
Clinical Isolates ◽  
Alternative Methods ◽  
Antibiofilm Activity ◽  
E Coli ◽  
Acetone Extracts

Bacterial biofilm is a complex community of bacterial cells enclosed in a polymer matrix and attached to a biotic or abiotic substrate. In this living form the bacteria are more resistant to antimicrobial agents than in the form of planktonic cells. Biofilm is a common cause of chronic infections in humans, so due to the growing resistance to antibiotics, alternative methods for controlling infections using medicinal plants have been proposed. In this study, the antibiofilm activity of ethanol and acetone extracts of plants Lamium album, Achillea millefolium and Agrimonia eupatoria against eight clinical isolates of human pathogenic bacteria was examined. Inhibition of biofilm formation was demonstrated using the crystal violet test and the effect on metabolic activity was confirmed by the use of resazurin dye test. Ethanol extract of L. album showed the greatest activity against P. aeruginosa (PA9) at a concentration of 20 mg/ml (> 80% of inhibition), while acetone extract acted at a concentration of 5 mg/ml (≥ 18%) against Klebsiella sp. (K9). At a concentration of 10 mg/ml, the ethanol extract of A. millefolium was effective against E. coli (E16) and P. aeruginosa (PA8) (> 70%), while the acetone extract was effective at 2.5 mg/ml (> 80%) against E. coli (E16). Ethanol and acetone extracts of A. eupatoria were effective at a concentration of 10 mg/ml (> 50%) against E. coli (E16). The antibiofilm activity of the tested plant extracts on certain clinical isolates indicates their great potential in the treatment of infections caused by biofilm-producing bacteria.

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