Sticky-flares for in situ monitoring of human telomerase RNA in living cells

Nanoscale ◽  
2018 ◽  
Vol 10 (19) ◽  
pp. 9386-9392 ◽  
Author(s):  
Qilong Wu ◽  
Zhengjie Liu ◽  
Lei Su ◽  
Guangmei Han ◽  
Renyong Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Telomerase Rna ◽  
Human Telomerase

A novel DNA-conjugated AuNP probe termed sticky-flares has been designed for the in situ detection of intracellular human telomerase RNA.

scholarly journals Elucidation of interfacial pH behaviour at the cell/substrate nanogap for in situ monitoring of cellular respiration

Nanoscale ◽  
2018 ◽  
Vol 10 (21) ◽  
pp. 10130-10136 ◽  
Author(s):  
Hiroto Satake ◽  
Akiko Saito ◽  
Toshiya Sakata
Keyword(s):  
Cellular Metabolism ◽  
Living Cells ◽  
In Situ Monitoring ◽  
Cellular Respiration ◽  
Cell Substrate

In situ monitoring of cellular metabolism is useful for elucidating dynamic functions of living cells.

scholarly journals Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal

2004 ◽  
Vol 164 (5) ◽  
pp. 647-652 ◽  
Author(s):  
Beáta E. Jády ◽  
Edouard Bertrand ◽  
Tamás Kiss
Keyword(s):  
S Phase ◽  
Cajal Body ◽  
Sequence Motif ◽  
Telomerase Rna ◽  
Dna Repeats ◽  
Telomeric Dna ◽  
Specific Localization ◽  
Localization Signal ◽  
Human Telomerase

Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here, fluorescence in situ hybridization demonstrates that in HeLa cancer cells, human telomerase RNA (hTR) accumulates in the nucleoplasmic Cajal bodies (CBs). Localization of transiently expressed hTR to CBs is supported by a short sequence motif (411-UGAG-414) that is located in the 3′-terminal box H/ACA RNA-like domain of hTR and that is structurally and functionally indistinguishable from the CB-specific localization signal of box H/ACA small CB-specific RNAs. In synchronized HeLa cells, hTR shows the most efficient accumulation in CBs during S phase, when telomeres are most likely synthesized. CBs may function in post-transcriptional maturation (e.g., cap hypermethylation of hTR), but they may also play a role in the assembly and/or function of telomerase holoenzyme.

Detection of the Human Telomerase RNA Component by in Situ Hybridization in Cells from Body Fluids

2005 ◽  
Vol 49 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Shin Nieh ◽  
Su-Feng Chen ◽  
Earl Fu ◽  
Chi-Ing Jan ◽  
Wei-Hua Lee
Keyword(s):  
In Situ Hybridization ◽  
Body Fluids ◽  
Telomerase Rna ◽  
Human Telomerase ◽  
In Cells

3D bimetallic Au/Pt nanoflowers decorated needle-type microelectrode for direct in situ monitoring of ATP secreted from living cells

2020 ◽  
Vol 153 ◽  
pp. 112019 ◽  
Author(s):  
Qin Zhu ◽  
Bo Liang ◽  
Yitao Liang ◽  
Lin Ji ◽  
Yu Cai ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Needle Type

Real-Time and In-Situ Monitoring of H2O2 Release from Living Cells by a Stretchable Electrochemical Biosensor Based on Vertically Aligned Gold Nanowires

2019 ◽  
Vol 91 (21) ◽  
pp. 13521-13527 ◽  
Author(s):  
Quanxia Lyu ◽  
Qingfeng Zhai ◽  
Jennifer Dyson ◽  
Shu Gong ◽  
Yunmeng Zhao ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Biosensor ◽  
Living Cells ◽  
In Situ Monitoring ◽  
Gold Nanowires ◽  
Vertically Aligned

In situ imaging of intracellular human telomerase RNA with molecular beacon-functionalized gold nanoparticles

2020 ◽  
Vol 12 (18) ◽  
pp. 2385-2390
Author(s):  
Tao Xie ◽  
Ziyan Fan ◽  
Ruilong Zhang ◽  
Xiaohe Tian ◽  
Guangmei Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Molecular Beacon ◽  
Live Cells ◽  
Telomerase Rna ◽  
Functionalized Gold Nanoparticles ◽  
Rna Imaging ◽  
Human Telomerase ◽  
In Situ Imaging

We develop molecular beacon-functionalized gold nanoparticles for in situ human telomerase RNA imaging in live cells.

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