In situ imaging of intracellular human telomerase RNA with molecular beacon-functionalized gold nanoparticles

2020 ◽  
Vol 12 (18) ◽  
pp. 2385-2390
Author(s):  
Tao Xie ◽  
Ziyan Fan ◽  
Ruilong Zhang ◽  
Xiaohe Tian ◽  
Guangmei Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Molecular Beacon ◽  
Live Cells ◽  
Telomerase Rna ◽  
Functionalized Gold Nanoparticles ◽  
Rna Imaging ◽  
Human Telomerase ◽  
In Situ Imaging

We develop molecular beacon-functionalized gold nanoparticles for in situ human telomerase RNA imaging in live cells.

scholarly journals Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

2019 ◽  
Vol 91 (3) ◽  
pp. 1719-1723 ◽  
Author(s):  
Yujing Zuo ◽  
Yu Zhang ◽  
Baoli Dong ◽  
Zhiming Gou ◽  
Tingxin Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
Binding Reaction ◽  
In Situ Imaging

scholarly journals An enzyme-free molecular catalytic device: dynamically self-assembled DNA dendrimers for in situ imaging of microRNAs in live cells

2019 ◽  
Vol 10 (6) ◽  
pp. 1651-1658 ◽  
Author(s):  
Shuzhen Yue ◽  
Xinyue Song ◽  
Weiling Song ◽  
Sai Bi
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Live Cells ◽  
Sensitivity And Selectivity ◽  
Catalytic Hairpin Assembly ◽  
Self Assembled ◽  
In Situ Imaging

Branched catalytic hairpin assembly is proposed for dynamic self-assembly of DNA dendrimers for signal amplification and in situ imaging of microRNAs in live cells with high sensitivity and selectivity.

scholarly journals Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal

2004 ◽  
Vol 164 (5) ◽  
pp. 647-652 ◽  
Author(s):  
Beáta E. Jády ◽  
Edouard Bertrand ◽  
Tamás Kiss
Keyword(s):  
S Phase ◽  
Cajal Body ◽  
Sequence Motif ◽  
Telomerase Rna ◽  
Dna Repeats ◽  
Telomeric Dna ◽  
Specific Localization ◽  
Localization Signal ◽  
Human Telomerase

Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here, fluorescence in situ hybridization demonstrates that in HeLa cancer cells, human telomerase RNA (hTR) accumulates in the nucleoplasmic Cajal bodies (CBs). Localization of transiently expressed hTR to CBs is supported by a short sequence motif (411-UGAG-414) that is located in the 3′-terminal box H/ACA RNA-like domain of hTR and that is structurally and functionally indistinguishable from the CB-specific localization signal of box H/ACA small CB-specific RNAs. In synchronized HeLa cells, hTR shows the most efficient accumulation in CBs during S phase, when telomeres are most likely synthesized. CBs may function in post-transcriptional maturation (e.g., cap hypermethylation of hTR), but they may also play a role in the assembly and/or function of telomerase holoenzyme.

Sticky-flares for in situ monitoring of human telomerase RNA in living cells

Nanoscale ◽  
2018 ◽  
Vol 10 (19) ◽  
pp. 9386-9392 ◽  
Author(s):  
Qilong Wu ◽  
Zhengjie Liu ◽  
Lei Su ◽  
Guangmei Han ◽  
Renyong Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Telomerase Rna ◽  
Human Telomerase

A novel DNA-conjugated AuNP probe termed sticky-flares has been designed for the in situ detection of intracellular human telomerase RNA.

In situ imaging and interfering Dicer-mediated cleavage process via a versatile molecular beacon probe

2019 ◽  
Vol 1079 ◽  
pp. 146-152 ◽  
Author(s):  
Kai Zhang ◽  
Xue-Jiao Yang ◽  
Ting-Ting Zhang ◽  
Xiang-Ling Li ◽  
Hong-Yuan Chen ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Cleavage Process ◽  
In Situ Imaging

Detection of the Human Telomerase RNA Component by in Situ Hybridization in Cells from Body Fluids

2005 ◽  
Vol 49 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Shin Nieh ◽  
Su-Feng Chen ◽  
Earl Fu ◽  
Chi-Ing Jan ◽  
Wei-Hua Lee
Keyword(s):  
In Situ Hybridization ◽  
Body Fluids ◽  
Telomerase Rna ◽  
Human Telomerase ◽  
In Cells
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