scholarly journals Elucidation of interfacial pH behaviour at the cell/substrate nanogap for in situ monitoring of cellular respiration

Nanoscale ◽  
2018 ◽  
Vol 10 (21) ◽  
pp. 10130-10136 ◽  
Author(s):  
Hiroto Satake ◽  
Akiko Saito ◽  
Toshiya Sakata
Keyword(s):  
Cellular Metabolism ◽  
Living Cells ◽  
In Situ Monitoring ◽  
Cellular Respiration ◽  
Cell Substrate

In situ monitoring of cellular metabolism is useful for elucidating dynamic functions of living cells.

Experiments in Nanomechanical Properties of Live Osteoblast Cells and Cell–Biomaterial Interface

2011 ◽  
Vol 2 (4) ◽  
Author(s):  
Rohit Khanna ◽  
Kalpana S. Katti ◽  
Dinesh R. Katti
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Structure ◽  
Living Cells ◽  
Actin Stress Fiber ◽  
Cell Culture Medium ◽  
Nanomechanical Properties ◽  
Cell Substrate ◽  
Significant Difference

Characterizing the mechanical characteristics of living cells and cell–biomaterial composite is an important area of research in bone tissue engineering. In this work, an in situ displacement-controlled nanoindentation technique (using Hysitron Triboscope) is developed to perform nanomechanical characterization of living cells (human osteoblasts) and cell–substrate constructs under physiological conditions (cell culture medium; 37 °C). In situ elastic moduli (E) of adsorbed proteins on tissue culture polystyrene (TCPS) under cell culture media were found to be ∼4 GPa as revealed by modulus mapping experiments. The TCPS substrates soaked in cell culture medium showed significant difference in surface nanomechanical properties (up to depths of ∼12 nm) as compared to properties obtained from deeper indentations. Atomic force microscopy (AFM) revealed the cytoskeleton structures such as actin stress fiber networks on flat cells which are believed to impart the structural integrity to cell structure. Load-deformation response of cell was found to be purely elastic in nature, i.e., cell recovers its shape on unloading as indicated by linear loading and unloading curves obtained at 1000 nm indentation depth. The elastic response of cells is obtained during initial cell adhesion (ECell, 1 h, 1000 nm = 4.4–12.4 MPa), cell division (ECell, 2 days, 1000 nm = 1.3–3.0 MPa), and cell spreading (ECell, 2 days, 1000 nm = 6.9–11.6 MPa). Composite nanomechanical responses of cell–TCPS constructs were obtained by indentation at depths of 2000 nm and 3000 nm on cell-seeded TCPS. Elastic properties of cell–substrate composites were mostly dominated by stiff TCPS (EBulk = 5 GPa) lying underneath the cell.

Sticky-flares for in situ monitoring of human telomerase RNA in living cells

Nanoscale ◽  
2018 ◽  
Vol 10 (19) ◽  
pp. 9386-9392 ◽  
Author(s):  
Qilong Wu ◽  
Zhengjie Liu ◽  
Lei Su ◽  
Guangmei Han ◽  
Renyong Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Telomerase Rna ◽  
Human Telomerase

A novel DNA-conjugated AuNP probe termed sticky-flares has been designed for the in situ detection of intracellular human telomerase RNA.

3D bimetallic Au/Pt nanoflowers decorated needle-type microelectrode for direct in situ monitoring of ATP secreted from living cells

2020 ◽  
Vol 153 ◽  
pp. 112019 ◽  
Author(s):  
Qin Zhu ◽  
Bo Liang ◽  
Yitao Liang ◽  
Lin Ji ◽  
Yu Cai ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Needle Type

Real-Time and In-Situ Monitoring of H2O2 Release from Living Cells by a Stretchable Electrochemical Biosensor Based on Vertically Aligned Gold Nanowires

2019 ◽  
Vol 91 (21) ◽  
pp. 13521-13527 ◽  
Author(s):  
Quanxia Lyu ◽  
Qingfeng Zhai ◽  
Jennifer Dyson ◽  
Shu Gong ◽  
Yunmeng Zhao ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Biosensor ◽  
Living Cells ◽  
In Situ Monitoring ◽  
Gold Nanowires ◽  
Vertically Aligned

An activatable AIEgen probe for in-situ monitoring and long-term tracking of ferrous ions in living cells

2021 ◽  
pp. 109271
Author(s):  
Liu Yang ◽  
Qingxin Chen ◽  
Shenglong Gan ◽  
Qiang Guo ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
In Situ Monitoring ◽  
Ferrous Ions
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