A PET-based turn-on fluorescent probe for sensitive detection of thiols and H2S and its bioimaging application in living cells, tissues and zebrafish

2019 ◽  
Vol 43 (7) ◽  
pp. 2865-2869 ◽  
Author(s):  
Kaixin Xu ◽  
Longwei He ◽  
Yunzhen Yang ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Sensitive Detection ◽  
Two Photon ◽  
Turn On

A two-photon turn-on fluorescent probe regulated by a PET mechanism has been engineered for detection of thiols and H2S in living cells, tissues and zebrafish.

A two-photon fluorescent probe for selective methylglyoxal detection and application in living cells

2015 ◽  
Vol 7 (6) ◽  
pp. 2386-2390 ◽  
Author(s):  
Tun Tang ◽  
Yimin Zhou ◽  
Yuqi Chen ◽  
Mengjun Li ◽  
Yan Feng ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Two Photon ◽  
Turn On

A novel two-photon fluorescent “turn-on” probe could detect methylglyoxal levels in living cells conveniently.

scholarly journals A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaohua Ma ◽  
Guoguang Wu ◽  
Yuehua Zhao ◽  
Zibo Yuan ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Coumarin Derivative ◽  
Living Cells ◽  
Sensitive Detection ◽  
Aqueous Environment ◽  
Test Probe ◽  
Aqueous Buffer ◽  
Turn On

We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.

Imaging nucleus viscosity and G-quadruplex DNA in living cells using a nucleus-targeting two-photon fluorescent probe

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5799-5804 ◽  
Author(s):  
Wan Sun ◽  
Jing-Xue Cui ◽  
Le-Le Ma ◽  
Zhong-Lin Lu ◽  
Bing Gong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Dna Detection ◽  
Living Cells ◽  
Cell Nuclei ◽  
Quadruplex Dna ◽  
Two Photon ◽  
Turn On ◽  
G Quadruplex

TP-2Bz, as a nucleus targeting “D–π–A” molecule, was developed as a two-photon turn-on fluorescent probe for the viscosity and GQ DNA detection in cell nuclei.

A fast-response two-photon fluorescent probe for the detection of Cys over GSH/Hcy with a large turn-on signal and its application in living tissues

2017 ◽  
Vol 5 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Jian-Yong Wang ◽  
Zhan-Rong Liu ◽  
Mingguang Ren ◽  
Xiuqi Kong ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fast Response ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Living Tissues

We developed a novel two-photon fluorescent probe for sensing Cys specifically. The two-photon fluorescent probe exhibited favorable properties and was successfully applied for imaging Cys in living cells and tissues.

A turn-on two-photon fluorescent probe for detecting lysosomal hydroxyl radicals in living cells

2019 ◽  
Vol 284 ◽  
pp. 744-750 ◽  
Author(s):  
Carlos Benitez-Martin ◽  
Juan A. Guadix ◽  
John R. Pearson ◽  
Francisco Najera ◽  
Jose M. Perez-Pomares ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hydroxyl Radicals ◽  
Living Cells ◽  
Two Photon ◽  
Turn On

A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells

2016 ◽  
Vol 8 (11) ◽  
pp. 2420-2426 ◽  
Author(s):  
Youming Shen ◽  
Xiangyang Zhang ◽  
Youyu Zhang ◽  
Chunxiang Zhang ◽  
Junling Jin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Optical Sensing ◽  
Living Cells ◽  
Sensitive Detection ◽  
Turn On ◽  
Dual Channel ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A novel and simple turn-on fluorescent probe bearing NBD and benzaldehyde fragments for the rapid optical sensing of biothiols has been designed and synthesized.

Development of a two-photon turn-on fluorescent probe for cysteine and its bio-imaging applications in living cells, tissues, and zebrafish

2018 ◽  
Vol 42 (17) ◽  
pp. 14075-14078 ◽  
Author(s):  
Shiying Gao ◽  
Yonghe Tang ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Bio Imaging ◽  
In Cells

A two-photon fluorescent probe Co-Cys for detecting cysteine has been designed to monitor cysteine in cells, tissues and zebrafish.

A turn-on near-infrared fluorescent probe with rapid response and large Stokes shift for the selective and sensitive detection of zinc(ii) and its application in living cells

2019 ◽  
Vol 11 (18) ◽  
pp. 2396-2403 ◽  
Author(s):  
Weixia Zhang ◽  
Xinxin Jin ◽  
Wei Chen ◽  
Chunhui Jiang ◽  
Hongfei Lu
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Near Infrared ◽  
Limit Of Detection ◽  
Stokes Shift ◽  
Living Cells ◽  
Rapid Response ◽  
Sensitive Detection ◽  
Large Stokes Shift ◽  
Turn On

Herein, a new near-infrared turn-on fluorescent probe, YPT, was designed and synthesized for the rapid detection of Zn2+, which exhibited low limit of detection for Zn2+ (12 nM) as well as a favorable large Stokes shift (λem − λex = 168 nm).

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