Two-photon microscopy of living cells by simultaneously exciting multiple endogenous fluorophores and fluorescent proteins

2010 ◽  
Author(s):  
Wei Zheng ◽  
Dong Li ◽  
Jianan Y. Qu
Keyword(s):  
Fluorescent Proteins ◽  
Living Cells ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Endogenous Fluorophores

A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

2020 ◽  
Vol 44 (26) ◽  
pp. 11342-11348 ◽  
Author(s):  
Ming-Xuan Hou ◽  
Liu-Yi Liu ◽  
Kang-Nan Wang ◽  
Xi-Juan Chao ◽  
Rong-Xue Liu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Molecular Rotor ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon fluorescent probe was developed for detecting mitochondrial viscosity during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) with good selectivity and highly biocompatible.

scholarly journals A new approach to dual-color two-photon microscopy with fluorescent proteins

2010 ◽  
Vol 10 (1) ◽  
pp. 6 ◽  
Author(s):  
Shane E Tillo ◽  
Thomas E Hughes ◽  
Nikolay S Makarov ◽  
Aleks Rebane ◽  
Mikhail Drobizhev
Keyword(s):  
Fluorescent Proteins ◽  
New Approach ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dual Color

Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy

2011 ◽  
Vol 156 (1) ◽  
pp. 410-415 ◽  
Author(s):  
Lin Xue ◽  
Zhangjian Fang ◽  
Guoping Li ◽  
Huanhuan Wang ◽  
Hua Jiang
Keyword(s):  
Aqueous Solution ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Zinc Ions ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High throughput instrument to screen fluorescent proteins under two-photon excitation

2020 ◽  
Author(s):  
Rosana S. Molina ◽  
Jonathan King ◽  
Jacob Franklin ◽  
Nathan Clack ◽  
Christopher McRaven ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Agar Plate ◽  
Focal Volume ◽  
E Coli ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

AbstractTwo-photon microscopy together with fluorescent proteins and fluorescent protein-based biosensors are commonly used tools in neuroscience. To enhance their experimental scope, it is important to optimize fluorescent proteins for two-photon excitation. Directed evolution of fluorescent proteins under one-photon excitation is common, but many one-photon properties do not correlate with two-photon properties. A simple system for expressing fluorescent protein mutants is E. coli colonies on an agar plate. The small focal volume of two-photon excitation makes creating a high throughput screen in this system a challenge for a conventional point-scanning approach. We present an instrument and accompanying software that solves this challenge by selectively scanning each colony based on a colony map captured under one-photon excitation. This instrument, called the GIZMO, can measure the two-photon excited fluorescence of 10,000 E. coli colonies in 7 hours. We show that the GIZMO can be used to evolve a fluorescent protein under two-photon excitation.

A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells

2018 ◽  
Vol 42 (18) ◽  
pp. 14725-14728 ◽  
Author(s):  
Ling Hu ◽  
Sajid Hussain ◽  
Tianyan Liu ◽  
Yuanzhen Yue ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Molecular Probes ◽  
Living Systems ◽  
Molecular Probe ◽  
Imidazole Derivatives ◽  
Two Photon Microscopy ◽  
Two Photon

Multi-functional florescent dyes capable of acting as molecular probes in living systems under two-photon microscopy, as well as super-resolution nanoscopy, are of great interest.

scholarly journals Bridging scales in scattering tissues via multifocal two-photon microscopy

2020 ◽  
Author(s):  
David Chen ◽  
Fabian Segovia-Miranda ◽  
Noreen Walker ◽  
Jose I. Valenzuela ◽  
Marino Zerial ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Large Scale ◽  
Fluorescent Proteins ◽  
Morphological Changes ◽  
Optical Techniques ◽  
Tissue Clearing ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Subcellular Resolution

Imaging biological systems at subcellular resolution and across scales is essential to under-standing how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue-clearing methods that cause significant morphological changes, compromise the integrity of cell membranes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that enables imaging mesoscopic scattering samples in their native tissue environment at high resolution and high speed.

scholarly journals Visualizing lipid structure and raft domains in living cells with two-photon microscopy

2003 ◽  
Vol 100 (26) ◽  
pp. 15554-15559 ◽  
Author(s):  
K. Gaus ◽  
E. Gratton ◽  
E. P. W. Kable ◽  
A. S. Jones ◽  
I. Gelissen ◽  
...  
Keyword(s):  
Living Cells ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lipid Structure ◽  
Raft Domains

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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