Gold nanoparticles and reduced graphene oxide-amplified label-free DNA biosensor for dasatinib detection

2018 ◽  
Vol 42 (19) ◽  
pp. 16378-16383 ◽  
Author(s):  
Fahimeh Tahernejad-Javazmi ◽  
Mehdi Shabani-Nooshabadi ◽  
Hassan Karimi-Maleh
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Adverse Effects ◽  
Reduced Graphene Oxide ◽  
The Other ◽  
Myelogenous Leukemia ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

Dasatinib or sprycel is an anticancer drug for treatment of chronic myelogenous leukemia, prostate cancer, and some of the other cancers with several adverse effects.

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing

2014 ◽  
Vol 60 ◽  
pp. 161-166 ◽  
Author(s):  
Zhao Zhang ◽  
Liqiang Luo ◽  
Guifang Chen ◽  
Yaping Ding ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

scholarly journals Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 87
Author(s):  
Yasmin Alhamoud ◽  
Yingying Li ◽  
Haibo Zhou ◽  
Ragwa Al-Wazer ◽  
Yiying Gong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Ochratoxin A ◽  
Fetal Malformation ◽  
Label Free ◽  
One Pot ◽  
Au Nps ◽  
Reduced Graphene ◽  
Relative Standard

Ochratoxin A (OTA) primarily obtained by the genera aspergillus and penicillium, is one of the toxic substances for different organs and systems of the human body such as the kidney, liver, neurons and the immune system. Moreover, it is considered to cause tumors and fetal malformation even at a very low concentration. Fast and sensitive assay for detection of OTA at ultralow levels in foods and agricultural products has been an increasing demand. In this study, a new label-free electrochemical biosensor based on three-dimensional reduced graphene oxide/gold nanoparticles/aptamer for OTA detection was constructed. The 3D-rGO/Au NPs nanocomposites were firstly synthesized using a one-pot hydrothermal process under optimized experimental conditions. The 3D-rGO/Au NPs with considerable particular surface area and outstanding electrical conductivity was then coated on a glass carbon electrode to provide tremendous binding sites for -SH modified aptamer via the distinctive Au–S linkage. The presence of OTA was specifically captured by aptamer and resulted in electrochemical impedance spectroscopy (EIS) signal response accordingly. The constructed impedimetric aptasensor obtained a broad linear response from 1 pg/mL to 10 ng/mL with an LOD of 0.34 pg/mL toward OTA detection, highlighting the excellent sensitivity. Satisfactory reproducibility was also achieved with the relative standard deviation (RSD) of 1.393%. Moreover, the proposed aptasensor obtained a good recovery of OTA detection in red wine samples within the range of 93.14 to 112.75% along with a low LOD of 0.023 ng/mL, indicating its applicability for OTA detection in real samples along with economical, specific, susceptible, fast, easy, and transportable merits.

scholarly journals Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection

2015 ◽  
Vol 72 ◽  
pp. 313-319 ◽  
Author(s):  
Bing Li ◽  
Genhua Pan ◽  
Neil D. Avent ◽  
Roy B. Lowry ◽  
Tracey E. Madgett ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Dna Detection ◽  
Label Free ◽  
Graphene Electrode ◽  
Free Dna ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide
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