A label-free DNA reduced graphene oxide-based fluorescent sensor for highly sensitive and selective detection of hemin

2011 ◽  
Vol 47 (16) ◽  
pp. 4676 ◽  
Author(s):  
Yan Shi ◽  
Wei Tao Huang ◽  
Hong Qun Luo ◽  
Nian Bing Li
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Fluorescent Sensor ◽  
Selective Detection ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene ◽  
Highly Sensitive

scholarly journals Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4936 ◽  
Author(s):  
Eliska Sedlackova ◽  
Zuzana Bytesnikova ◽  
Eliska Birgusova ◽  
Pavel Svec ◽  
Amir M. Ashrafi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Free Dna ◽  
Methylation Assay ◽  
Reduced Graphene

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing

2014 ◽  
Vol 60 ◽  
pp. 161-166 ◽  
Author(s):  
Zhao Zhang ◽  
Liqiang Luo ◽  
Guifang Chen ◽  
Yaping Ding ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

Facile synthesis of novel reduced graphene oxide@polystyrene nanospheres for sensitive label-free electrochemical immunoassay

2020 ◽  
Vol 56 (5) ◽  
pp. 699-702 ◽  
Author(s):  
Qingchun Lan ◽  
Huifang Shen ◽  
Juan Li ◽  
Chuanli Ren ◽  
Xiaoya Hu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Immunoassay ◽  
Label Free ◽  
Facile Synthesis ◽  
Polystyrene Nanospheres ◽  
Reduced Graphene ◽  
Highly Sensitive

Nanosized reduced graphene oxide@polystyrene nanospheres were first synthesized and further exploited for highly sensitive label-free electrochemical immunoassay applications.

Highly sensitive and selective detection of Bis-phenol A based on hydroxyapatite decorated reduced graphene oxide nanocomposites

2017 ◽  
Vol 241 ◽  
pp. 353-361 ◽  
Author(s):  
Mohammad K. Alam ◽  
Mohammed M. Rahman ◽  
Amir Elzwawy ◽  
Sri Ramulu Torati ◽  
Mohammad S. Islam ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Selective Detection ◽  
Reduced Graphene ◽  
Highly Sensitive

scholarly journals Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection

2015 ◽  
Vol 72 ◽  
pp. 313-319 ◽  
Author(s):  
Bing Li ◽  
Genhua Pan ◽  
Neil D. Avent ◽  
Roy B. Lowry ◽  
Tracey E. Madgett ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Dna Detection ◽  
Label Free ◽  
Graphene Electrode ◽  
Free Dna ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A three dimensional Pt nanodendrite/graphene/MnO2 nanoflower modified electrode for the sensitive and selective detection of dopamine

2015 ◽  
Vol 3 (37) ◽  
pp. 7440-7448 ◽  
Author(s):  
Beibei Yang ◽  
Jin Wang ◽  
Duan Bin ◽  
Mingshan Zhu ◽  
Ping Yang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Sensor ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Three Dimensional ◽  
Selective Detection ◽  
Ternary Composite ◽  
Reduced Graphene ◽  
Highly Sensitive

A highly sensitive and selective electrochemical sensor: a 3D hierarchical ternary composite including a Pt nanodendrite/reduced graphene oxide/MnO2 nanoflower modified electrode.

Gold nanoparticles and reduced graphene oxide-amplified label-free DNA biosensor for dasatinib detection

2018 ◽  
Vol 42 (19) ◽  
pp. 16378-16383 ◽  
Author(s):  
Fahimeh Tahernejad-Javazmi ◽  
Mehdi Shabani-Nooshabadi ◽  
Hassan Karimi-Maleh
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Adverse Effects ◽  
Reduced Graphene Oxide ◽  
The Other ◽  
Myelogenous Leukemia ◽  
Label Free ◽  
Free Dna ◽  
Reduced Graphene

Dasatinib or sprycel is an anticancer drug for treatment of chronic myelogenous leukemia, prostate cancer, and some of the other cancers with several adverse effects.

scholarly journals Label-free impedimetric sensing platform for microRNA-21 based on ZrO2-reduced graphene oxide nanohybrids coupled with catalytic hairpin assembly amplification

RSC Advances ◽  
2018 ◽  
Vol 8 (29) ◽  
pp. 16146-16151 ◽  
Author(s):  
Keying Zhang ◽  
Na Zhang ◽  
Li Zhang ◽  
Hongyan Wang ◽  
Hongwei Shi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Sensitive Detection ◽  
Label Free ◽  
Assembly Strategy ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly ◽  
Highly Sensitive Detection

A label-free impedimetric sensor for highly sensitive detection of miRNA-21 based on ZrO2-RGO nanocomposite and a catalytic hairpin assembly strategy.

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