Low cost 3D microfluidic chips for multiplex protein detection based on photonic crystal beads

Lab on a Chip ◽  
2018 ◽  
Vol 18 (23) ◽  
pp. 3638-3644 ◽  
Author(s):  
Ning Chang ◽  
Jingyan Zhai ◽  
Bing Liu ◽  
Jiping Zhou ◽  
Zhaoyu Zeng ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Microfluidic Chip ◽  
Low Cost ◽  
Three Dimensional ◽  
Protein Detection ◽  
Microfluidic Chips

A low-cost three dimensional (3D) microfluidic chip was fabricated and integrated with photonic crystal beads for protein detection and multiplex bioassays.

Study on the Manufacture of Microfluidic Chip with Coated Glass

2012 ◽  
Vol 548 ◽  
pp. 254-257 ◽  
Author(s):  
Yan He ◽  
Bai Ling Huang ◽  
Yong Lai Zhang ◽  
Li Gang Niu
Keyword(s):  
Microfluidic Chip ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Critical Factor ◽  
Wet Etching ◽  
Microfluidic Chips ◽  
Etching Technique ◽  
Chip Quality ◽  
Wet Chemical ◽  
Wet Chemical Etching

In this paper, a simple and facile technique for manufacturing glass-based microfluidic chips was developed. Instead of using expensive dry etching technology, the standard UV lithography and wet chemical etching technique was used to fabricate microchannels on a K9 glass substrate. The fabrication process of microfluidic chip including vacuum evaporation, annealing, lithography, and BHF (HF-NH4F-H2O) wet etching were investigated. Through series experiments, we found that anneal was the critical factor for chip quality. As a representative example, a microfluidic channel with 20 m of depth, and 80 m of width was successfully prepared, and the channel surfaces are quite smooth. These results present a simple, low cost, flexible and easy way to fabricate glass-based microfluidic chips.

Design and Fabrication of Micro Hot Embossing Mold for Microfluidic Chip Used in Flow Cytometry

2007 ◽  
Vol 339 ◽  
pp. 246-251
Author(s):  
L.Q. Du ◽  
C. Liu ◽  
H.J. Liu ◽  
J. Qin ◽  
N. Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Chip ◽  
Low Cost ◽  
Hot Embossing ◽  
Microfluidic Chips ◽  
Metal Mold ◽  
Nickel Electroforming ◽  
Thick Photoresist ◽  
Nickel Base ◽  
Microfabrication Technology

Micro hot embossing mold of microfluidic chip used in flow cytometry is designed and microfabricated. After some kinds of microfabrication processes are tried, this paper presents a novel microfabrication technology of micro hot embossing metal mold. Micro metal mold is fabricated by low-cost UV-LIGA surface micro fabrication process using negative thick photoresist, SU-8. Different from other micro hot embossing molds, the micro mold with vertical sidewalls is fabricated by micro nickel electroforming directly on Nickel base. Based on the micro Nickel mold and automation fabrication system, high precision and mass-producing microfluidic chips have been fabricated and they have been used in flow cytometry

scholarly journals Portable all-in-one automated microfluidic system (PAMICON) with 3D-printed chip using novel fluid control mechanism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yushen Zhang ◽  
Tsun-Ming Tseng ◽  
Ulf Schlichtmann
Keyword(s):  
Active Control ◽  
Microfluidic Chip ◽  
Control Mechanism ◽  
State Of The Art ◽  
Low Cost ◽  
Microfluidic System ◽  
Microfluidic Chips ◽  
Microfluidic Systems ◽  
3D Printed ◽  
Pdms Chip

AbstractState-of-the-art microfluidic systems rely on relatively expensive and bulky off-chip infrastructures. The core of a system—the microfluidic chip—requires a clean room and dedicated skills to be fabricated. Thus, state-of-the-art microfluidic systems are barely accessible, especially for the do-it-yourself (DIY) community or enthusiasts. Recent emerging technology—3D-printing—has shown promise to fabricate microfluidic chips more simply, but the resulting chip is mainly hardened and single-layered and can hardly replace the state-of-the-art Polydimethylsiloxane (PDMS) chip. There exists no convenient fluidic control mechanism yet suitable for the hardened single-layered chip, and particularly, the hardened single-layered chip cannot replicate the pneumatic valve—an essential actuator for automatically controlled microfluidics. Instead, 3D-printable non-pneumatic or manually actuated valve designs are reported, but their application is limited. Here, we present a low-cost accessible all-in-one portable microfluidic system, which uses an easy-to-print single-layered 3D-printed microfluidic chip along with a novel active control mechanism for fluids to enable more applications. This active control mechanism is based on air or gas interception and can, e.g., block, direct, and transport fluid. As a demonstration, we show the system can automatically control the fluid in microfluidic chips, which we designed and printed with a consumer-grade 3D-printer. The system is comparably compact and can automatically perform user-programmed experiments. All operations can be done directly on the system with no additional host device required. This work could support the spread of low budget accessible microfluidic systems as portable, usable on-the-go devices and increase the application field of 3D-printed microfluidic devices.

Direct-writing colloidal photonic crystal microfluidic chips by inkjet printing for label-free protein detection

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3089 ◽  
Author(s):  
Weizhi Shen ◽  
Mingzhu Li ◽  
Changqing Ye ◽  
Lei Jiang ◽  
Yanlin Song
Keyword(s):  
Photonic Crystal ◽  
Inkjet Printing ◽  
Protein Detection ◽  
Microfluidic Chips ◽  
Label Free ◽  
Direct Writing ◽  
Free Protein

scholarly journals Assessing the Reusability of 3D-Printed Photopolymer Microfluidic Chips for Urine Processing

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 520 ◽  
Author(s):  
Eric Lepowsky ◽  
Reza Amin ◽  
Savas Tasoglu
Keyword(s):  
Protein Adsorption ◽  
Microfluidic Device ◽  
Low Cost ◽  
Three Dimensional ◽  
Device Fabrication ◽  
Microfluidic Chips ◽  
3D Printed ◽  
Printed Materials ◽  
Urine Processing ◽  
Nonspecific Protein Adsorption

Three-dimensional (3D) printing is emerging as a method for microfluidic device fabrication boasting facile and low-cost fabrication, as compared to conventional fabrication approaches, such as photolithography, for poly(dimethylsiloxane) (PDMS) counterparts. Additionally, there is an increasing trend in the development and implementation of miniaturized and automatized devices for health monitoring. While nonspecific protein adsorption by PDMS has been studied as a limitation for reusability, the protein adsorption characteristics of 3D-printed materials have not been well-studied or characterized. With these rationales in mind, we study the reusability of 3D-printed microfluidics chips. Herein, a 3D-printed cleaning chip, consisting of inlets for the sample, cleaning solution, and air, and a universal outlet, is presented to assess the reusability of a 3D-printed microfluidic device. Bovine serum albumin (BSA) was used a representative urinary protein and phosphate-buffered solution (PBS) was chosen as the cleaning agent. Using the 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) fluorescence detection method, the protein cross-contamination between samples and the protein uptake of the cleaning chip were assessed, demonstrating a feasible 3D-printed chip design and cleaning procedure to enable reusable microfluidic devices. The performance of the 3D-printed cleaning chip for real urine sample handling was then validated using a commercial dipstick assay.

scholarly journals A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 624 ◽  
Author(s):  
Trieu Nguyen ◽  
Aaydha Chidambara Vinayaka ◽  
Dang Duong Bang ◽  
Anders Wolff
Keyword(s):  
Salmonella Enteritidis ◽  
Solid Phase ◽  
Point Of Care ◽  
Low Cost ◽  
Three Dimensional ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Microfluidic Chips ◽  
Industrial Companies ◽  
Polymerase Chain

This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).

scholarly journals Photonic Crystal Stimuli-Responsive Chromatic Sensors: A Short Review

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 290 ◽  
Author(s):  
Andrea Chiappini ◽  
Lam Thi Ngoc Tran ◽  
Pablo Marco Trejo-García ◽  
Lidia Zur ◽  
Anna Lukowiak ◽  
...  
Keyword(s):  
Refractive Index ◽  
Photonic Crystal ◽  
Electromagnetic Waves ◽  
Low Cost ◽  
Physical Phenomenon ◽  
Spectral Characteristics ◽  
Three Dimensional ◽  
Short Review ◽  
Optical Measurements ◽  
Energy Harvest

Photonic crystals (PhC) are spatially ordered structures with lattice parameters comparable to the wavelength of propagating light. Their geometrical and refractive index features lead to an energy band structure for photons, which may allow or forbid the propagation of electromagnetic waves in a limited frequency range. These unique properties have attracted much attention for both theoretical and applied research. Devices such as high-reflection omnidirectional mirrors, low-loss waveguides, and high- and low-reflection coatings have been demonstrated, and several application areas have been explored, from optical communications and color displays to energy harvest and sensors. In this latter area, photonic crystal fibers (PCF) have proven to be very suitable for the development of highly performing sensors, but one-dimensional (1D), two-dimensional (2D) and three-dimensional (3D) PhCs have been successfully employed, too. The working principle of most PhC sensors is based on the fact that any physical phenomenon which affects the periodicity and the refractive index of the PhC structure induces changes in the intensity and spectral characteristics of the reflected, transmitted or diffracted light; thus, optical measurements allow one to sense, for instance, temperature, pressure, strain, chemical parameters, like pH and ionic strength, and the presence of chemical or biological elements. In the present article, after a brief general introduction, we present a review of the state of the art of PhC sensors, with particular reference to our own results in the field of mechanochromic sensors. We believe that PhC sensors based on changes of structural color and mechanochromic effect are able to provide a promising, technologically simple, low-cost platform for further developing devices and functionalities.

scholarly journals A fast and simple bonding method for low cost microfluidic chip fabrication

2018 ◽  
Vol 69 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Zhifu Yin ◽  
Helin Zou
Keyword(s):  
Microfluidic Chip ◽  
Low Cost ◽  
Adhesive Tape ◽  
Microfluidic Chips ◽  
Bonding Pressure ◽  
Gas Leakage ◽  
Microstructure Fabrication ◽  
Chip Fabrication ◽  
Commercial Applications ◽  
Bonding Method

Abstract With the development of the microstructure fabrication technique, microfluidic chips are widely used in biological and medical researchers. Future advances in their commercial applications depend on the mass bonding of microfluidic chip. In this study we are presenting a simple, low cost and fast way of bonding microfluidic chips at room temperature. The influence of the bonding pressure on the deformation of the microchannel and adhesive tape was analyzed by numerical simulation. By this method, the microfluidic chip can be fully sealed at low temperature and pressure without using any equipment. The dye water and gas leakage test indicated that the microfluidic chip can be bonded without leakage or block and its bonding strength can up to 0.84 MPa.

Fabrication of 3D Temperature Sensor Using Magnetostrictive Inkjet Printhead

2020 ◽  
Vol 64 (5) ◽  
pp. 50405-1-50405-5
Author(s):  
Young-Woo Park ◽  
Myounggyu Noh
Keyword(s):  
Flexible Electronics ◽  
Inkjet Printing ◽  
Temperature Sensor ◽  
Computer Aided Design ◽  
Low Cost ◽  
Three Dimensional ◽  
Drop On Demand ◽  
Aided Design ◽  
Nanoparticle Ink ◽  
Inkjet Printhead

Abstract Recently, the three-dimensional (3D) printing technique has attracted much attention for creating objects of arbitrary shape and manufacturing. For the first time, in this work, we present the fabrication of an inkjet printed low-cost 3D temperature sensor on a 3D-shaped thermoplastic substrate suitable for packaging, flexible electronics, and other printed applications. The design, fabrication, and testing of a 3D printed temperature sensor are presented. The sensor pattern is designed using a computer-aided design program and fabricated by drop-on-demand inkjet printing using a magnetostrictive inkjet printhead at room temperature. The sensor pattern is printed using commercially available conductive silver nanoparticle ink. A moving speed of 90 mm/min is chosen to print the sensor pattern. The inkjet printed temperature sensor is demonstrated, and it is characterized by good electrical properties, exhibiting good sensitivity and linearity. The results indicate that 3D inkjet printing technology may have great potential for applications in sensor fabrication.

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