scholarly journals Scalable production of double emulsion drops with thin shells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1936-1942 ◽  
Author(s):  
A. Vian ◽  
B. Reuse ◽  
E. Amstad
Keyword(s):  
High Throughput ◽  
Shell Thickness ◽  
Double Emulsion ◽  
Thin Shells ◽  
Double Emulsions ◽  
Emulsion Drops ◽  
Scalable Production

The microfluidic aspiration device reduces the shell thickness of double emulsions down to 240 nm at a high throughput.

scholarly journals Parallelizable microfluidic dropmakers with multilayer geometry for the generation of double emulsions

Lab on a Chip ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Saraf Nawar ◽  
Joshuah K. Stolaroff ◽  
Congwang Ye ◽  
Huayin Wu ◽  
Du Thai Nguyen ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Microfluidic Devices ◽  
Surface Wettability ◽  
Double Emulsions ◽  
Channel Surface ◽  
Emulsion Drops ◽  
Scalable Production

We present a multilayer dropmaker geometry that enables the modular fabrication of microfluidic devices containing precisely patterned channel surface wettability. The platform is used for the scalable production of uniform double emulsion drops.

scholarly journals Generation of Ultra-Thin-Shell Microcapsules Using Osmolarity-Controlled Swelling Method

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 444 ◽  
Author(s):  
Jianhua Guo ◽  
Lihua Hou ◽  
Junpeng Hou ◽  
Jiali Yu ◽  
Qingming Hu
Keyword(s):  
Thin Shell ◽  
Shell Thickness ◽  
Double Emulsion ◽  
Spherical Shape ◽  
Thin Shells ◽  
Biomolecular Sensing ◽  
Oil In Water ◽  
Emulsion Drops ◽  
Long Term Observation

Microcapsules are attractive core-shell configurations for studies of controlled release, biomolecular sensing, artificial microbial environments, and spherical film buckling. However, the production of microcapsules with ultra-thin shells remains a challenge. Here we develop a simple and practical osmolarity-controlled swelling method for the mass production of monodisperse microcapsules with ultra-thin shells via water-in-oil-in-water (W/O/W) double-emulsion drops templating. The size and shell thickness of the double-emulsion drops are precisely tuned by changing the osmotic pressure between the inner cores and the suspending medium, indicating the practicability and effectiveness of this swelling method in tuning the shell thickness of double-emulsion drops and the resultant microcapsules. This method enables the production of microcapsules even with an ultra-thin shell less than hundreds of nanometers, which overcomes the difficulty in producing ultra-thin-shell microcapsules using the classic microfluidic emulsion technologies. In addition, the ultra-thin-shell microcapsules can maintain their intact spherical shape for up to 1 year without rupturing in our long-term observation. We believe that the osmolarity-controlled swelling method will be useful in generating ultra-thin-shell polydimethylsiloxane (PDMS) microcapsules for long-term encapsulation, and for thin film folding, buckling and rupturing investigation.

Double-emulsion drops with ultra-thin shells for capsule templates

Lab on a Chip ◽  
2011 ◽  
Vol 11 (18) ◽  
pp. 3162-3166 ◽  
Author(s):  
Shin-Hyun Kim ◽  
Jin Woong Kim ◽  
Jun-Cheol Cho ◽  
David A. Weitz
Keyword(s):  
Double Emulsion ◽  
Thin Shells ◽  
Emulsion Drops

scholarly journals Synchronized Reagent Delivery in Double Emulsions for Triggering Chemical Reactions and Gene Expression

2021 ◽  
Author(s):  
Ariane Stucki ◽  
Petra Jusková ◽  
Nicola Nuti ◽  
Steven Schmitt ◽  
Petra Dittrich
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
High Throughput Screening ◽  
Double Emulsion ◽  
Lipid Vesicles ◽  
Enzyme Engineering ◽  
Droplet Microfluidics ◽  
Enzymatic Reactions ◽  
High Throughput Analysis ◽  
Double Emulsions

Microfluidic methods to form single emulsion and double emulsion (DE) droplets have greatly enhanced the toolbox for high throughput screening for cell or enzyme engineering and drug discovery. However, remaining challenges in the supply of reagents into these enclosed nanoliter compartments limit the applicability of droplet microfluidics. Here, we introduce a strategy for on-demand delivery of reactants in DEs. We use lipid vesicles as transport carriers, which are co-encapsulated in double emulsions and release their cargo upon addition of an external trigger, here the anionic surfactant SDS. The reagent present inside the lipid vesicles stays isolated from the remaining content of the DE vessel until SDS enters the DE lumen and solubilizes the lipid bilayer. We demonstrate the versatility of the method with two critical applications, chosen as representative assays for high throughput screening. First, we trigger enzymatic reactions after releasing a reactant and second, we encapsulate bacteria and induce gene expression at a delayed time. The presented technique is compatible with the high throughput analysis of individual DE droplets using conventional flow cytometry as well as with microfluidic time-resolved studies. The possibility of delaying and controlling reagent delivery in current high throughput compartmentalization systems will significantly extend their range of applications e.g. for directed evolution, and further improve their compatibility with biological systems.

scholarly journals Synchronized Reagent Delivery in Double Emulsions for Triggering Chemical Reactions and Gene Expression

2021 ◽  
Author(s):  
Ariane Stucki ◽  
Petra Jusková ◽  
Nicola Nuti ◽  
Steven Schmitt ◽  
Petra Dittrich
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
High Throughput Screening ◽  
Double Emulsion ◽  
Lipid Vesicles ◽  
Enzyme Engineering ◽  
Droplet Microfluidics ◽  
Enzymatic Reactions ◽  
High Throughput Analysis ◽  
Double Emulsions

Microfluidic methods to form single emulsion and double emulsion (DE) droplets have greatly enhanced the toolbox for high throughput screening for cell or enzyme engineering and drug discovery. However, remaining challenges in the supply of reagents into these enclosed nanoliter compartments limit the applicability of droplet microfluidics. Here, we introduce a strategy for on-demand delivery of reactants in DEs. We use lipid vesicles as transport carriers, which are co-encapsulated in double emulsions and release their cargo upon addition of an external trigger, here the anionic surfactant SDS. The reagent present inside the lipid vesicles stays isolated from the remaining content of the DE vessel until SDS enters the DE lumen and solubilizes the lipid bilayer. We demonstrate the versatility of the method with two critical applications, chosen as representative assays for high throughput screening. First, we trigger enzymatic reactions after releasing a reactant and second, we encapsulate bacteria and induce gene expression at a delayed time. The presented technique is compatible with the high throughput analysis of individual DE droplets using conventional flow cytometry as well as with microfluidic time-resolved studies. The possibility of delaying and controlling reagent delivery in current high throughput compartmentalization systems will significantly extend their range of applications e.g. for directed evolution, and further improve their compatibility with biological systems.

scholarly journals Correction: Double-emulsion drops with ultra-thin shells for capsule templates

Lab on a Chip ◽  
2017 ◽  
Vol 17 (3) ◽  
pp. 567-567 ◽  
Author(s):  
Shin-Hyun Kim ◽  
Jin Woong Kim ◽  
Jun-Cheol Cho ◽  
David A. Weitz
Keyword(s):  
Double Emulsion ◽  
Thin Shells ◽  
Emulsion Drops

Correction for ‘Double-emulsion drops with ultra-thin shells for capsule templates’ by Shin-Hyun Kim et al., Lab Chip, 2011, 11, 3162–3166.

scholarly journals Single-Round Remodeling of the Active Site of an Artificial Metalloenzyme using an Ultrahigh-Throughput Double Emulsion Screening Assay

2021 ◽  
Author(s):  
Jaicy Vallapurackal ◽  
Ariane Stucki ◽  
Alexandria Deliz Liang ◽  
Juliane Klehr ◽  
Petra S Dittrich ◽  
...  
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Double Emulsion ◽  
Cell Encapsulation ◽  
Optimization Procedure ◽  
Droplet Microfluidics ◽  
Screening Assay ◽  
Fluorescent Intensity ◽  
Double Emulsions ◽  
Artificial Metalloenzyme

The potential of high-throughput compartmentalization renders droplet microfluidics an attractive tool for directed evolution of enzymes as it permits maintenance of the phenotype-genotype linkage throughout the entire optimization procedure. In particular, water-in-oil-in-water double emulsions droplets (DEs) produced by microfluidics enable the analysis of reaction compartments at ultra-high-throughput using commercially available fluorescence-activated cell sorting (FACS) devices. Here we report a streamlined method applicable for the ultrahigh-throughput screening of an artificial metalloenzyme (ArM), an artificial deallylase (ADAse), in double emulsions. The DE-protocol was validated by screening a four hundred member, double-mutant streptavidin library for the CpRu-catalyzed uncaging of aminocoumarin. The most active variants, identified by next-generation sequencing of the sorted DE droplets with highest fluorescent intensity, are in good agreement with 96-well plate screening hits. These findings, thus, pave the way towards the systematic implementation of commercially available FACS for the directed evolution of metalloenzymes making ultrahigh-throughput screening more broadly accessible. The use of microfluidics for the formation of uniform compartments with precise control over reagents and cell encapsulation further facilitates the establishment of highly reliable quantitative assays.

scholarly journals Cross-talk between emulsion drops: how are hydrophilic reagents transported across oil phases?

Lab on a Chip ◽  
2018 ◽  
Vol 18 (24) ◽  
pp. 3903-3912 ◽  
Author(s):  
Gianluca Etienne ◽  
Antoine Vian ◽  
Marjan Biočanin ◽  
Bart Deplancke ◽  
Esther Amstad
Keyword(s):  
Cross Talk ◽  
Shell Thickness ◽  
Cross Contamination ◽  
Double Emulsions ◽  
Emulsion Drops

We demonstrate that small aqueous drops form in surfactant-containing oils that are in contact with aqueous phases. These drops transport even large reagents across oil shells of double emulsions, resulting in cross-contamination. This can be reduced by using appropriate surfactants or by reducing the shell thickness below 1 μm.

scholarly journals High-Throughput Production of Micrometer Sized Double Emulsions and Microgel Capsules in Parallelized 3D Printed Microfluidic Devices

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1887 ◽  
Author(s):  
Alexander Jans ◽  
Jonas Lölsberg ◽  
Abdolrahman Omidinia-Anarkoli ◽  
Robin Viermann ◽  
Martin Möller ◽  
...  
Keyword(s):  
Surface Modification ◽  
High Throughput ◽  
Hollow Sphere ◽  
Double Emulsion ◽  
Continuous Phase ◽  
Flow Focusing ◽  
Double Emulsions ◽  
Emulsion Droplets ◽  
3D Printed ◽  
Double Emulsion Droplets

Double emulsions are useful geometries as templates for core-shell particles, hollow sphere capsules, and for the production of biomedical delivery vehicles. In microfluidics, two approaches are currently being pursued for the preparation of microfluidic double emulsion devices. The first approach utilizes soft lithography, where many identical double-flow-focusing channel geometries are produced in a hydrophobic silicone matrix. This technique requires selective surface modification of the respective channel sections to facilitate alternating wetting conditions of the channel walls to obtain monodisperse double emulsion droplets. The second technique relies on tapered glass capillaries, which are coaxially aligned, so that double emulsions are produced after flow focusing of two co-flowing streams. This technique does not require surface modification of the capillaries, as only the continuous phase is in contact with the emulsifying orifice; however, these devices cannot be fabricated in a reproducible manner, which results in polydisperse double emulsion droplets, if these capillary devices were to be parallelized. Here, we present 3D printing as a means to generate four identical and parallelized capillary device architectures, which produce monodisperse double emulsions with droplet diameters in the range of 500 µm. We demonstrate high throughput synthesis of W/O/W and O/W/O double emulsions, without the need for time-consuming surface treatment of the 3D printed microfluidic device architecture. Finally, we show that we can apply this device platform to generate hollow sphere microgels.

Sign in / Sign up

Export Citation Format

Share Document