A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamers

Lab on a Chip ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 1633-1640 ◽  
Author(s):  
Jia-Han Wu ◽  
Chih-Hung Wang ◽  
Yu-Dong Ma ◽  
Gwo-Bin Lee
Keyword(s):  
Acinetobacter Baumannii ◽  
Composite Membrane ◽  
Microfluidic System ◽  
Magnetic Composite ◽  
Nitrocellulose Membrane ◽  
Bacterial Detection ◽  
Detection Process

A new, nitrocellulose-based microfluidic system featuring Acinetobacter baumannii (AB)-specific aptamers capable of automating the bacterial detection process was reported.

Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1210-1222 ◽  
Author(s):  
Ting-Hang Liu ◽  
Shu-Shen Cheng ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Limit Of Detection ◽  
Microfluidic System ◽  
Joint Fluid ◽  
Bacterial Detection ◽  
Detection And Identification ◽  
System P ◽  
Colony Forming Units ◽  
Synovial Fluids ◽  
Human Joint

An integrated microfluidic system was developed for detecting and identifying four bacteria in human joint fluid with the limit of detection as low as 100 colony forming units (CFUs) per milliliter (or 20 CFUs per reaction).

scholarly journals Correction: Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

The Analyst ◽  
2019 ◽  
Vol 144 (19) ◽  
pp. 5898-5898
Author(s):  
Ting-Hang Liu ◽  
Shu-Shen Cheng ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Microfluidic System ◽  
Bacterial Detection ◽  
Detection And Identification ◽  
System P ◽  
Synovial Fluids

Correction for ‘Bacterial detection and identification from human synovial fluids on an integrated microfluidic system’ by Ting-Hang Liu et al., Analyst, 2019, 144, 1210–1222.

scholarly journals Preparation and Characterization of Fe3O4/PVDF Magnetic Composite Membrane

2004 ◽  
Vol 20 (06) ◽  
pp. 598-601 ◽  
Author(s):  
Du Jun ◽  
◽  
Wu Ling ◽  
Tao Chang-Yuan ◽  
Sun Cai-Xin
Keyword(s):  
Composite Membrane ◽  
Magnetic Composite

An automatic integrated microfluidic system for allergy microarray chips

The Analyst ◽  
2018 ◽  
Vol 143 (10) ◽  
pp. 2285-2292 ◽  
Author(s):  
Wen-Yen Huang ◽  
Shang-Ta Chou ◽  
Chia-Hui Chen ◽  
Shan-Ying Chou ◽  
Jia-Han Wu ◽  
...  
Keyword(s):  
Microfluidic System ◽  
Integrated System ◽  
Detection Process ◽  
Allergen Detection ◽  
Microarray Chips

An integrated system combining microfluidic techniques and microarray chips was developed to automate the allergen detection process.

Amidolytic Detection of Prothrombin Activation Products after SDS-Gel Electrophoresis

1989 ◽  
Vol 61 (03) ◽  
pp. 386-391 ◽  
Author(s):  
Guido Tans ◽  
Truus Janssen-Claessen ◽  
Jan Rosing
Keyword(s):  
Gel Electrophoresis ◽  
Activated Protein C ◽  
Factor Xa ◽  
Coagulation Factors ◽  
Product Formation ◽  
Chromogenic Substrate ◽  
Nitrocellulose Membrane ◽  
Activation Products ◽  
Factor Xia ◽  
Prothrombin Activation

SummaryIn this paper we report a method via which enzymatically active products formed during prothrombin activation can be detected by simple photographic means after SDS-gel electrophoresis, blotting onto a nitrocellulose membrane and visualization with the chromogenic substrate, S2238. After amidolytic detection the same nitrocellulose membrane can also be used for immunologic detection of prothrombin activation products, thus allowing a complete description of product formation during prothrombin activation.The detection limit of the so-called “amidoblot” is approximately 3 ng thrombin per gel sample which is comparable to the sensitivity of immunoblotting.It is further shown that the amidoblot technique can also be applied to other coagulation factors for which a suitable chromogenic substrate is available (factor XIIa, kallikrein, factor XIa, factor Xa, plasmin and activated protein C).

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