Glucose oxidation performance is improved by the use of a supramolecular self-assembly of glucose oxidase and catalase

2019 ◽  
Vol 9 (2) ◽  
pp. 477-482 ◽  
Author(s):  
Caixia Cui ◽  
Yunming Fang ◽  
Biqiang Chen ◽  
Tianwei Tan

A multi-enzymes complex was assembled successfully via host/guest interactions, and it was used for rapid transformation of glucose.

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 993 ◽  
Author(s):  
Baoyan Wu ◽  
Shihua Hou ◽  
Yongyong Xue ◽  
Zhan Chen

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.


2006 ◽  
Vol 41 (10) ◽  
pp. 3147-3149 ◽  
Author(s):  
Xin Hua Xu ◽  
Guang Lei Ren ◽  
Juan Cheng ◽  
Qiang Liu ◽  
Dong Guang Li ◽  
...  

2019 ◽  
Vol 275 ◽  
pp. 197-205 ◽  
Author(s):  
Wei Zhuang ◽  
Jinsha Huang ◽  
Xiaojing Liu ◽  
Lei Ge ◽  
Huanqing Niu ◽  
...  

2001 ◽  
Vol 73 (6) ◽  
pp. 1161-1168 ◽  
Author(s):  
E. J. Calvo ◽  
R. Etchenique ◽  
L. Pietrasanta ◽  
A. Wolosiuk ◽  
C. Danilowicz

2015 ◽  
Vol 285 ◽  
pp. 493-498 ◽  
Author(s):  
Sidney Aquino Neto ◽  
Ross D. Milton ◽  
Laís B. Crepaldi ◽  
David P. Hickey ◽  
Adalgisa R. de Andrade ◽  
...  

2003 ◽  
Vol 8 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Yasuo Nakabayashi ◽  
Kazuyo Nakamura ◽  
Mayuko Kawachi ◽  
Takeshi Motoyama ◽  
Osamu Yamauchi

Langmuir ◽  
1997 ◽  
Vol 13 (10) ◽  
pp. 2708-2716 ◽  
Author(s):  
Jose Hodak ◽  
Roberto Etchenique ◽  
Ernesto J. Calvo ◽  
Kavita Singhal ◽  
Philip N. Bartlett

2004 ◽  
Vol 37 (8) ◽  
pp. 1012-1018 ◽  
Author(s):  
Makoto Yoshimoto ◽  
Shaoqing Wang ◽  
Yoshihiro Arimatsu ◽  
Keiji Furumoto ◽  
Kimitoshi Fukunaga ◽  
...  

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