Layer-by-Layer Self-Assembly of Glucose Oxidase with a Poly(allylamine)ferrocene Redox Mediator

Langmuir ◽  
1997 ◽  
Vol 13 (10) ◽  
pp. 2708-2716 ◽  
Author(s):  
Jose Hodak ◽  
Roberto Etchenique ◽  
Ernesto J. Calvo ◽  
Kavita Singhal ◽  
Philip N. Bartlett
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Redox Mediator ◽  
Layer By Layer

Layer by layer self-assembly immobilization of glucose oxidase onto chitosan-graft-polyaniline polymers

2006 ◽  
Vol 41 (10) ◽  
pp. 3147-3149 ◽  
Author(s):  
Xin Hua Xu ◽  
Guang Lei Ren ◽  
Juan Cheng ◽  
Qiang Liu ◽  
Dong Guang Li ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Layer By Layer

Layer-By-Layer Self-Assembly of Glucose Oxidase and Os(Bpy)2ClPyCH2NH−poly(Allylamine) Bioelectrode

2001 ◽  
Vol 73 (6) ◽  
pp. 1161-1168 ◽  
Author(s):  
E. J. Calvo ◽  
R. Etchenique ◽  
L. Pietrasanta ◽  
A. Wolosiuk ◽  
C. Danilowicz
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Layer By Layer

Efficient Immobilization of Glucose Oxidase on Nanocomposite via Layer-by-Layer Self-Assembly to Construct Bionanomultilayer Films

2012 ◽  
Vol 160 (1) ◽  
pp. H6-H12 ◽  
Author(s):  
Lingyan Jin ◽  
Xia Gao ◽  
Huan Chen ◽  
Lisha Wang ◽  
Qi Wu ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Layer By Layer

scholarly journals Development of an Effective and Economic Biosensor for Diabetic Blood Monitoring Based on MWCNTs, Artificial Redox Mediator Ferrocene, Nafion Polymer and a Local Extracted and Purified Glucose Oxidase Enzyme from Penicillium Notatum F-158 Fungus

2021 ◽  
Author(s):  
Hanaa El-Desoky ◽  
Ashraf Koleeb ◽  
Roqaya Bassuiny ◽  
Tarek Mohamed
Keyword(s):  
Glucose Oxidase ◽  
Redox Mediator ◽  
Enzymatic Oxidation ◽  
Layer By Layer ◽  
Plasma Samples ◽  
Penicillium Notatum ◽  
Glucose Determination ◽  
Fruit Samples ◽  
Oxidase Enzyme ◽  
Orange Fruit

Abstract Enhancement of the properties of glucose oxidase (GOx) enzyme is still receiving attention due to its extensive applications. Eight different fungal strains were isolated from soil and orange fruit samples for inexpensive GOx production. Penicillium notatum F-158 (P. notatum) strain produced a remarkable amount of GOx. Its culture condition was optimized for optimum GOx production. GOx was purified and its activity, stability and kinetic parameters were studied. An effective biosensor {GCE/[MWCNTs–Fc–GOx(FAD)–NF]} based on layer by layer immbolization of MWCNTs, aritificial ferrocene (Fc) redox mediator, extracted P. notatum GOx enzyme and nafion polymer (NF) on glassy carbon electrode (GCE) surface was developed for glucose determination. Fc acts as an electron relay between enzyme and MWCNTs/GCE. The synergy of MWCNTs and Fc enhances the electrocatalytic action of Fc to the enzymatic oxidation of glucose. Low potential (+0.3V) of Fc applied in amperometric measurements avoids interference of the main electroactive substances present in the real plasma samples. This biosensor showed broad linear ranges {2.80×10-4 to 14.99×10-3 M} and low detection limit (8.68×10-6 M) for glucose determination. The achieved glucose concentrations in six plasma samples are consistent with normal values in human blood indicating that such biosensor could be used clinically.

scholarly journals Construction of Chitosan/Alginate Nano-Drug Delivery System for Improving Dextran Sodium Sulfate-Induced Colitis in Mice

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1884
Author(s):  
Mengfei Jin ◽  
Shangyong Li ◽  
Yanhong Wu ◽  
Dandan Li ◽  
Yantao Han
Keyword(s):  
Self Assembly ◽  
Average Diameter ◽  
Mucosal Healing ◽  
Plga Nanoparticles ◽  
Narrow Particle Size Distribution ◽  
Layer By Layer ◽  
Systemic Side Effects ◽  
Assembly Technology ◽  
The Fourier Transform ◽  
Polyelectrolyte Film

(1) Background: In the treatment of ulcerative colitis (UC), accurate delivery and release of anti-inflammatory drugs to the site of inflammation can reduce systemic side effects. (2) Methods: We took advantage of this goal to prepare resveratrol-loaded PLGA nanoparticles (RES-PCAC-NPs) by emulsification solvent volatilization. After layer-by-layer self-assembly technology, we deposited chitosan and alginate to form a three-layer polyelectrolyte film. (3) Results: It can transport nanoparticles through the gastric environment to target inflammation sites and slowly release drugs at a specific pH. The resulting RES-PCAC-NPs have an ideal average diameter (~255 nm), a narrow particle size distribution and a positively charged surface charge (~13.5 mV). The Fourier transform infrared spectroscopy showed that resveratrol was successfully encapsulated into PCAC nanoparticles, and the encapsulation efficiency reached 87.26%. In addition, fluorescence imaging showed that RES-PCAC-NPs with positive charges on the surface can effectively target and accumulate in the inflammation site while continuing to penetrate downward to promote mucosal healing. Importantly, oral RES-PCAC-NPs treatment in DSS-induced mice was superior to other results in significantly improved inflammatory markers of UC. (4) Conclusions: Our results strongly prove that RES-PCAC-NPs can target the inflamed colon for maximum efficacy, and this oral pharmaceutical formulation can represent a promising formulation in the treatment of UC.

Graphene-Based Multilayers Constructed from Layer-by-Layer Self-Assembly Techniques

2014 ◽  
Vol 14 (2) ◽  
pp. 1145-1153 ◽  
Author(s):  
Bing Yu ◽  
Xiaomian Liu ◽  
Hailin Cong ◽  
Hua Yuan ◽  
Dong Wang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Layer By Layer
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