scholarly journals Structural characterization of self-assemblies of new omega-3 lipids: docosahexaenoic acid and docosapentaenoic acid monoglycerides

2018 ◽  
Vol 20 (37) ◽  
pp. 23928-23941 ◽  
Author(s):  
Xianrong Shao ◽  
Gizem Bor ◽  
Sabah Al-Hosayni ◽  
Stefan Salentinig ◽  
Anan Yaghmur
Keyword(s):  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Structural Characterization ◽  
Docosapentaenoic Acid ◽  
Omega 3

Hexosomes based on omega-3 polyunsaturated fatty acid monoglycerides.

scholarly journals Two step-production of acylglycerols containing a high proportion of docosapentaenoic acid from marine omega-3 oil and their oxidative stability

2018 ◽  
Vol 4 ◽  
Author(s):  
Zhongshui Yu ◽  
Jiankang Wang ◽  
Fereidoon Shahidi
Keyword(s):  
Fatty Acid ◽  
Oxidative Stability ◽  
Polyunsaturated Fatty Acid ◽  
Time Course ◽  
Docosapentaenoic Acid ◽  
Fatty Acid Esters ◽  
Omega 3 ◽  
Seal Blubber Oil ◽  
Temperature Time ◽  
Acid Esters

The objective of this study was to concentrate polyunsaturated fatty acid (PUFA) from seal blubber oil in an innovative manner to produce a high content of docosapentaenoic acid (DPA) in the resultant product. It also aimed at investigating the use of lipases as catalysts for synthesizing acylglycerols from glycerol and polyunsaturated fatty acid concentrates. Additionally, study of the oxidative stability of acylglycerols synthesized by lipases was intended. A two-stage urea complexation process was used to concentrate PUFA from seal blubber oil, giving rise to a DPA content of up to 24.0% in the product. Enzymatic synthesis of acyglycerols directly from glycerol and fatty acid concentrate was studied. Three lipases were used as biocatalysts for esterification. Lipase SP435 from Candida antarctica showed the highest activity for esterification. Effects of reaction parameters, namely temperature, time course and mole ratio of glycerol to fatty acid were followed with all three lipases. The optimal reaction time was 24 hr at 30 °C at a mole ratio of glycerol to fatty acid of 14:1. The maximum degree of acylglycerol synthesis was > 90%. The effect of time course and mole ratio of glycerol to fatty acid on acylglycerols distribution was also determined. The oxidative stability of different samples under Schaal-oven conditions at 60 °C showed that the oxidative stability of acylglycerols was better than that of the corresponding fatty acid esters.

scholarly journals Effect of Nitrogen Sources on Omega-3 Polyunsaturated Fatty Acid Biosynthesis and Gene Expression in Thraustochytriidae sp.

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 612
Author(s):  
Siting Li ◽  
Zhangli Hu ◽  
Xuewei Yang ◽  
Yan Li
Keyword(s):  
Fatty Acid ◽  
Nitrogen Source ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Quantitative Polymerase Chain Reaction ◽  
Docosapentaenoic Acid ◽  
Nitrogen Sources ◽  
Omega 3 ◽  
Fatty Acid Elongase ◽  
Coa Ligase

The molecular mechanism that contributes to nitrogen source dependent omega-3 polyunsaturated fatty acid (n-3 PUFA) synthesis in marine oleaginous protists Thraustochytriidae sp., was explored in this study. The fatty acid (FA) synthesis was significantly influenced by the supplement of various levels of sodium nitrate (SN) (1–50 mM) or urea (1–50 mM). Compared with SN (50 mM) cultivation, cells from urea (50 mM) cultivation accumulated 1.16-fold more n-3 PUFAs (49.49% docosahexaenoic acid (DHA) (w/w, of total FAs) and 5.28% docosapentaenoic acid (DPA) (w/w, of total FAs)). Strikingly higher quantities of short chain FAs (<18 carbons) (52.22-fold of that in urea cultivation) were produced from SN cultivation. Ten candidate reference genes (RGs) were screened by using four statistical methods (geNorm, NormFinder, Bestkeeper and RefFinder). MFT (Mitochondrial folate transporter) and NUC (Nucleolin) were determined as the stable RGs to normalize the RT-qPCR (real-time quantitative polymerase chain reaction) data of essential genes related to n-3 PUFAs-synthesis. Our results elucidated that the gene transcripts of delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, enoyl-CoA hydratase, fatty acid elongase 3, long-chain fatty acid acyl-CoA ligase, and acetyl-CoA carboxylase were up-regulated under urea cultivation, contributing to the extension and unsaturated bond formation. These findings indicated that regulation of the specific genes through nitrogen source could greatly stimulate n-3 PUFA production in Thraustochytriidae sp.

scholarly journals Dietary Omega-3 Polyunsaturated Fatty Acid Deprivation Does Not Alter Seizure Thresholds but May Prevent the Anti-seizure Effects of Injected Docosahexaenoic Acid in Rats

2019 ◽  
Vol 9 ◽  
Author(s):  
Ameer Y. Taha ◽  
Marc-Olivier Trepanier ◽  
Flaviu A. Coibanu ◽  
Anjali Saxena ◽  
Melanie A. Jeffrey ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Omega 3

scholarly journals The Omega-3 Polyunsaturated Fatty Acid Docosahexaenoic Acid (DHA) Reverses Corticosterone-Induced Changes in Cortical Neurons

2015 ◽  
pp. pyv130 ◽  
Author(s):  
Matteo M. Pusceddu ◽  
Yvonne M. Nolan ◽  
Holly F. Green ◽  
Ruairi C. Robertson ◽  
Catherine Stanton ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Cortical Neurons ◽  
Omega 3 ◽  
Induced Changes

A high omega-3 fatty acid diet rapidly changes the lipid composition of cardiac tissue and results in cardioprotection

2018 ◽  
Vol 96 (9) ◽  
pp. 916-921 ◽  
Author(s):  
Mélissa Desnoyers ◽  
Kim Gilbert ◽  
Ness Madingou ◽  
Marc-André Gagné ◽  
Caroline Daneault ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Infarct Size ◽  
Lipid Composition ◽  
Cardiac Tissue ◽  
Docosapentaenoic Acid ◽  
The Other ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Omega 3 Fatty Acid

The present study was designed to ascertain the effects of 3 diets with different omega-3/6 fatty acid ratios on infarct size and the modifications that these diets induce in the lipid composition of cardiac tissue. Sprague-Dawley rats were fed omega-3/6 fatty acid diets with 1:1, 1:5, or 1:20 ratios for at least 10 days, followed by occlusion of the left anterior descending artery for 40 min and 24 h of reperfusion. Infarct size was significantly smaller in the 1:1 group than in the other groups. Significantly higher concentrations of the omega-3 fatty acids eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid were found in the 1:1 group than in the other groups. Omega-6 polyunsaturated fatty acid levels were similar between groups, although they were higher in the 1:5 and 1:20 groups than in the 1:1 group. Margaric acid concentrations were higher in the 1:1 group than in the other groups. Docosahexaenoic acid levels in cardiac tissue and infarct size were significantly correlated with no other significant links being apparent. The present study indicated that a 1:1 omega-3/6 fatty acid ratio protected against ischemia and was associated with increased omega-3 fatty acid composition of cardiac tissue.

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