Live imaging of cell membrane-localized MT1-MMP activity on a microfluidic chip

2018 ◽  
Vol 54 (81) ◽  
pp. 11435-11438 ◽  
Author(s):  
Nan Li ◽  
Weifei Zhang ◽  
Ling Lin ◽  
Ziyi He ◽  
Mashooq Khan ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
Microfluidic Chip ◽  
Live Imaging ◽  
Activatable Probe

We designed an enzyme-activatable probe for real time in situ tracking of MT1-MMP activity.

Real-time effects of Cd(ii) on the cellular membrane permeability

The Analyst ◽  
2021 ◽  
Vol 146 (19) ◽  
pp. 5973-5979
Author(s):  
Biao Zhang ◽  
Na Pan ◽  
Xiaoyin Fan ◽  
Liping Lu ◽  
Xiayan Wang
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Real Time ◽  
Cellular Membrane ◽  
Cell Membrane Permeability ◽  
Time Effects

Using SECM to determine the cell membrane permeability has the advantages of being real-time, in situ and sensitive. Compared with x-scan study, DPV technology shows a higher performance in dectecting changes in the membrane permeability.

A semisynthetic fluorescent protein assembly-based FRET probe for real-time profiling of cell membrane protease functions in situ

2019 ◽  
Vol 55 (15) ◽  
pp. 2218-2221 ◽  
Author(s):  
Sujuan Sun ◽  
Yanan Liu ◽  
Julan Xia ◽  
Miao Wang ◽  
Rui Tang ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
Fluorescent Protein ◽  
Protein Assembly

A semisynthetic fluorescent protein assembly-based FRET probe (sFPAP) was proposed for cell membrane protease function assay.

In Situ and Real-Time SFG Measurements Revealing Organization and Transport of Cholesterol Analogue 6-Ketocholestanol in a Cell Membrane

2014 ◽  
Vol 5 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Sulan Ma ◽  
Hongchun Li ◽  
Kangzhen Tian ◽  
Shuji Ye ◽  
Yi Luo
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
A Cell

Amide I SFG Spectral Line Width Probes the Lipid–Peptide and Peptide–Peptide Interactions at Cell Membrane In Situ and in Real Time

Langmuir ◽  
2018 ◽  
Vol 34 (25) ◽  
pp. 7554-7560 ◽  
Author(s):  
Baixiong Zhang ◽  
Junjun Tan ◽  
Chuanzhao Li ◽  
Jiahui Zhang ◽  
Shuji Ye
Keyword(s):  
Cell Membrane ◽  
Spectral Line ◽  
Real Time ◽  
Line Width ◽  
Spectral Line Width ◽  
Peptide Interactions

Novel Innate Immune Functions Revealed by Dynamic, Real-Time Live Imaging of Bacterial Infections

2010 ◽  
Vol 30 (2) ◽  
pp. 107-117 ◽  
Author(s):  
Keira Melican ◽  
Jorrit Boekel ◽  
Monica Ryden-Aulin ◽  
Agneta Richter-Dahlfors
Keyword(s):  
Real Time ◽  
Bacterial Infections ◽  
Live Imaging ◽  
Innate Immune ◽  
Immune Functions

Probing the Dynamic Self-Assembly Behaviour of Photoswitchable Wormlike Micelles in Real Time

2018 ◽  
Author(s):  
Elaine A. Kelly ◽  
Judith E. Houston ◽  
Rachel Evans
Keyword(s):  
Real Time ◽  
Absorption Spectroscopy ◽  
Self Assembly ◽  
Uv Light ◽  
Wormlike Micelles ◽  
Tetraethylene Glycol ◽  
Light Illumination ◽  
First Time ◽  
Dependent Processes

Understanding the dynamic self-assembly behaviour of azobenzene photosurfactants (AzoPS) is crucial to advance their use in controlled release applications such as<i></i>drug delivery and micellar catalysis. Currently, their behaviour in the equilibrium <i>cis-</i>and <i>trans</i>-photostationary states is more widely understood than during the photoisomerisation process itself. Here, we investigate the time-dependent self-assembly of the different photoisomers of a model neutral AzoPS, <a>tetraethylene glycol mono(4′,4-octyloxy,octyl-azobenzene) </a>(C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>) using small-angle neutron scattering (SANS). We show that the incorporation of <i>in-situ</i>UV-Vis absorption spectroscopy with SANS allows the scattering profile, and hence micelle shape, to be correlated with the extent of photoisomerisation in real-time. It was observed that C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>could switch between wormlike micelles (<i>trans</i>native state) and fractal aggregates (under UV light), with changes in the self-assembled structure arising concurrently with changes in the absorption spectrum. Wormlike micelles could be recovered within 60 seconds of blue light illumination. To the best of our knowledge, this is the first time the degree of AzoPS photoisomerisation has been tracked <i>in</i><i>-situ</i>through combined UV-Vis absorption spectroscopy-SANS measurements. This technique could be widely used to gain mechanistic and kinetic insights into light-dependent processes that are reliant on self-assembly.

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

Flat Flexible Thin Milli-electrode Array for Real-time in situ Water Quality Monitoring in Distribution Systems

2017 ◽  
Vol 2017 (4) ◽  
pp. 5598-5617
Author(s):  
Zhiheng Xu ◽  
Wangchi Zhou ◽  
Qiuchen Dong ◽  
Yan Li ◽  
Dingyi Cai ◽  
...  
Keyword(s):  
Water Quality ◽  
Real Time ◽  
Distribution Systems ◽  
Electrode Array ◽  
Water Quality Monitoring ◽  
Quality Monitoring
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