Real-time effects of Cd(ii) on the cellular membrane permeability

The Analyst ◽  
2021 ◽  
Vol 146 (19) ◽  
pp. 5973-5979
Author(s):  
Biao Zhang ◽  
Na Pan ◽  
Xiaoyin Fan ◽  
Liping Lu ◽  
Xiayan Wang
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Real Time ◽  
Cellular Membrane ◽  
Cell Membrane Permeability ◽  
Time Effects

Using SECM to determine the cell membrane permeability has the advantages of being real-time, in situ and sensitive. Compared with x-scan study, DPV technology shows a higher performance in dectecting changes in the membrane permeability.

Quantitative and real-time effects of carbon quantum dots on single living HeLa cell membrane permeability

Nanoscale ◽  
2014 ◽  
Vol 6 (10) ◽  
pp. 5116 ◽  
Author(s):  
Weiqian Kong ◽  
Juan Liu ◽  
Ruihua Liu ◽  
Hao Li ◽  
Yang Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Membrane ◽  
Hela Cell ◽  
Membrane Permeability ◽  
Real Time ◽  
Carbon Quantum Dots ◽  
Cell Membrane Permeability ◽  
Time Effects ◽  
Single Living

Size-dependent and real-time effect of SiO2 nanoparticles on a single living HeLa Cell's membrane permeability

2015 ◽  
Vol 3 (7) ◽  
pp. 1198-1203 ◽  
Author(s):  
Weiqian Kong ◽  
Hao Li ◽  
Juan Liu ◽  
SijieGuo SijieGuo ◽  
Yuzhi Han ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Real Time ◽  
Sio2 Nanoparticles ◽  
Time Effect ◽  
Cell Membrane Permeability ◽  
Size Dependent ◽  
Low Toxic ◽  
Single Living

Low toxic and biocompatible SiO2 NPs of different sizes show different effects on cell membrane permeability.

scholarly journals Biophysical insight into mechanisms of sonoporation

2016 ◽  
Vol 113 (36) ◽  
pp. 9983-9988 ◽  
Author(s):  
Brandon Helfield ◽  
Xucai Chen ◽  
Simon C. Watkins ◽  
Flordeliza S. Villanueva
Keyword(s):  
Shear Stress ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Real Time ◽  
Cellular Membrane ◽  
Ultrasound Frequency ◽  
Root Relation ◽  
Stress Threshold ◽  
Membrane Reorganization ◽  
Insight Into

This study presents a unique approach to understanding the biophysical mechanisms of ultrasound-triggered cell membrane disruption (i.e., sonoporation). We report direct correlations between ultrasound-stimulated encapsulated microbubble oscillation physics and the resulting cellular membrane permeability by simultaneous microscopy of these two processes over their intrinsic physical timescales (microseconds for microbubble dynamics and seconds to minutes for local macromolecule uptake and cell membrane reorganization). We show that there exists a microbubble oscillation-induced shear-stress threshold, on the order of kilopascals, beyond which endothelial cellular membrane permeability increases. The shear-stress threshold exhibits an inverse square-root relation to the number of oscillation cycles and an approximately linear dependence on ultrasound frequency from 0.5 to 2 MHz. Further, via real-time 3D confocal microscopy measurements, our data provide evidence that a sonoporation event directly results in the immediate generation of membrane pores through both apical and basal cell membrane layers that reseal along their lateral area (resealing time of ∼<2 min). Finally, we demonstrate the potential for sonoporation to indirectly initiate prolonged, intercellular gaps between adjacent, confluent cells (∼>30–60 min). This real-time microscopic approach has provided insight into both the physical, cavitation-based mechanisms of sonoporation and the biophysical, cell-membrane–based mechanisms by which microbubble acoustic behaviors cause acute and sustained enhancement of cellular and vascular permeability.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Synthesis of novel cationic spermine-conjugated phosphotriester oligonucleotide for improvement of cell membrane permeability

2015 ◽  
Vol 25 (17) ◽  
pp. 3610-3615 ◽  
Author(s):  
Junsuke Hayashi ◽  
Tomoko Hamada ◽  
Ikumi Sasaki ◽  
Osamu Nakagawa ◽  
Shun-ichi Wada ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability

scholarly journals Red Cell Membrane Permeability Deduced from Bulk Diffusion Coefficients

1974 ◽  
Vol 64 (6) ◽  
pp. 706-729 ◽  
Author(s):  
W. R. Redwood ◽  
E. Rall ◽  
W. Perl
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Diffusion Coefficients ◽  
Bulk Diffusion ◽  
Red Cells ◽  
Cell Membrane Permeability ◽  
Red Cell ◽  
Cell Column ◽  
Red Cell Membrane ◽  
One Dimensional

The permeability coefficients of dog red cell membrane to tritiated water and to a series of[14C]amides have been deduced from bulk diffusion measurements through a "tissue" composed of packed red cells. Red cells were packed by centrifugation inside polyethylene tubing. The red cell column was pulsed at one end with radiolabeled solute and diffusion was allowed to proceed for several hours. The distribution of radioactivity along the red cell column was measured by sequential slicing and counting, and the diffusion coefficient was determined by a simple plotting technique, assuming a one-dimensional diffusional model. In order to derive the red cell membrane permeability coefficient from the bulk diffusion coefficient, the red cells were assumed to be packed in a regular manner approximating closely spaced parallelopipeds. The local steady-state diffusional flux was idealized as a one-dimensional intracellular pathway in parallel with a one-dimensional extracellular pathway with solute exchange occurring within the series pathway and between the pathways. The diffusion coefficients in the intracellular and extracellular pathways were estimated from bulk diffusion measurements through concentrated hemoglobin solutions and plasma, respectively; while the volume of the extracellular pathway was determined using radiolabeled sucrose. The membrane permeability coefficients were in satisfactory agreement with the data of Sha'afi, R. I., C. M. Gary-Bobo, and A. K. Solomon (1971. J. Gen. Physiol. 58:238) obtained by a rapid-reaction technique. The method is simple and particularly well suited for rapidly permeating solutes.

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