Supramolecular nanoparticles based on β-CD modified hyaluronic acid for DNA encapsulation and controlled release

2018 ◽  
Vol 54 (63) ◽  
pp. 8713-8716 ◽  
Author(s):  
Yang Yang ◽  
Xin Jia ◽  
Ying-Ming Zhang ◽  
Nan Li ◽  
Yu Liu
Keyword(s):  
Hyaluronic Acid ◽  
Controlled Release ◽  
Supramolecular Nanoparticles ◽  
Dna Encapsulation ◽  
Positively Charged

Supramolecular nanoparticles composed of doubly positively charged adamantane (ADA2+) and β-CD modified hyaluronic acid (HACD) were constructed and the controlled binding and release of pDNA was realized.

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

scholarly journals Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1β-Induced Inflammation and Apoptosis in Chondrocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Bo Qiu ◽  
Ming Gong ◽  
Qi-Ting He ◽  
Pang-Hu Zhou
Keyword(s):  
Hyaluronic Acid ◽  
Controlled Release ◽  
Receptor Antagonist ◽  
Release Kinetics ◽  
Quantitative Polymerase Chain Reaction ◽  
Interleukin 1 ◽  
B Cell Lymphoma ◽  
Chondrocyte Apoptosis ◽  
Burst Release ◽  
Interleukin 1 Receptor Antagonist

This paper investigates the protective effect of interleukin-1 receptor antagonist (IL-1Ra) released from hyaluronic acid chitosan (HA-CS) microspheres in a controlled manner on IL-1β-induced inflammation and apoptosis in chondrocytes. The IL-1Ra release kinetics was characterized by an initial burst release, which was reduced to a linear release over eight days. Chondrocytes were stimulated with 10 ng/ml IL-1β and subsequently incubated with HA-CS-IL-1Ra microspheres. The cell viability was decreased by IL-1β, which was attenuated by HA-CS-IL-1Ra microspheres as indicated by an MTT assay. ELISA showed that HA-CS-IL-1Ra microspheres inhibited IL-1β-induced inflammation by attenuating increases in NO2- and prostaglandin E2 levels as well as increase in glycosaminoglycan release. A terminal deoxyribonucleotide transferase deoxyuridine triphosphate nick-end labeling assay revealed that the IL-1β-induced chondrocyte apoptosis was decreased by HA-CS-IL-1Ra microspheres. Moreover, HA-CS-IL-1Ra microspheres blocked IL-1β-induced chondrocyte apoptosis by increasing B-cell lymphoma 2 (Bcl-2) and decreasing Bcl-2-associated X protein and caspase-3 expressions at mRNA and protein levels, as indicated by reverse-transcription quantitative polymerase chain reaction and western blot analysis, respectively. The results of the present study indicated that HA-CS-IL-1Ra microspheres as a controlled release system of IL-1Ra possess potential anti-inflammatory and antiapoptotic properties in rat chondrocytes due to their ability to regulate inflammatory factors and apoptosis associated genes.

Controlled release behavior of bioactive molecules from photo-reactive hyaluronic acid-alginate scaffolds

2006 ◽  
Vol 14 (5) ◽  
pp. 530-538 ◽  
Author(s):  
Hye Sung Nam ◽  
Jeongho An ◽  
Dong June Chung ◽  
Ji-Heung Kim ◽  
Chong-Pyoung Chung
Keyword(s):  
Hyaluronic Acid ◽  
Controlled Release ◽  
Bioactive Molecules ◽  
Release Behavior

scholarly journals Development of Gelatin-Coated Microspheres for Novel Bioink Design

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3339
Author(s):  
Muskan Kanungo ◽  
Yale Wang ◽  
Noah Hutchinson ◽  
Emma Kroll ◽  
Anna DeBruine ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Flow Rate ◽  
Bioactive Molecules ◽  
Great Promise ◽  
Charged Surface ◽  
Design Expert ◽  
Gelatin Concentration ◽  
Gelatin Coating ◽  
Positively Charged

A major challenge in tissue engineering is the formation of vasculature in tissue and organs. Recent studies have shown that positively charged microspheres promote vascularization, while also supporting the controlled release of bioactive molecules. This study investigated the development of gelatin-coated pectin microspheres for incorporation into a novel bioink. Electrospray was used to produce the microspheres. The process was optimized using Design-Expert® software. Microspheres underwent gelatin coating and EDC catalysis modifications. The results showed that the concentration of pectin solution impacted roundness and uniformity primarily, while flow rate affected size most significantly. The optimal gelatin concentration for microsphere coating was determined to be 0.75%, and gelatin coating led to a positively charged surface. When incorporated into bioink, the microspheres did not significantly alter viscosity, and they distributed evenly in bioink. These microspheres show great promise for incorporation into bioink for tissue engineering applications.

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