DNA logic assembly powered by a triplex-helix molecular switch for extracellular pH imaging

2018 ◽  
Vol 54 (61) ◽  
pp. 8498-8501 ◽  
Author(s):  
Hongjie Qi ◽  
Shuzhen Yue ◽  
Sai Bi ◽  
Caifeng Ding ◽  
Weiling Song
Keyword(s):  
Self Assembly ◽  
Molecular Switch ◽  
Extracellular Ph ◽  
The Self ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Molecular Logic ◽  
Ph Imaging ◽  
Logic Operations

A strategy is rationally designed to finely control the hybridization chain reaction (HCR) for the self-assembly of DNA nanostructures by simply changing the pH, which is successfully applied to molecular logic operations and extracellular pH imaging.

Lab in a tube: a fast-assembled colorimetric sensor for highly sensitive detection of oligonucleotides based on a hybridization chain reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (55) ◽  
pp. 44714-44721 ◽  
Author(s):  
Siqi Zhang ◽  
Kun Wang ◽  
Zhenyu Li ◽  
Zhongmin Feng ◽  
Ting Sun
Keyword(s):  
Complex Formation ◽  
Dna Sequence ◽  
Self Assembly ◽  
The Self ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Colored Product ◽  
Highly Sensitive Detection

Upon adding THBV, the self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to accelerate H1–H2 complex formation. The G-quadruplex at the end of the H1–H2 complex could catalyze TMB into a colored product.

Triggered and catalyzed self-assembly of hyperbranched DNA structures for logic operations and homogeneous CRET biosensing of microRNA

2016 ◽  
Vol 52 (31) ◽  
pp. 5455-5458 ◽  
Author(s):  
Sai Bi ◽  
Shuzhen Yue ◽  
Qiang Wu ◽  
Jiayan Ye
Keyword(s):  
Self Assembly ◽  
Hybridization Chain Reaction ◽  
Strand Displacement ◽  
Chain Reaction ◽  
Dna Structures ◽  
Catalytic Hairpin Assembly ◽  
Displacement Based ◽  
Logic Operations

Toehold-mediated strand displacement-based nanocircuits are developed by integrating catalytic hairpin assembly with hybridization chain reaction, achieving self-assembly of hyperbranched DNA structures and amplified homogeneous CRET detection of microRNA.

scholarly journals Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Feng Li ◽  
Wenting Yu ◽  
Jiaojiao Zhang ◽  
Yuhang Dong ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Steric Effect ◽  
Sirna Delivery ◽  
Dna Nanotechnology ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Hairpins ◽  
In Vivo Experiments ◽  
Dna And Rna

AbstractDNA nanostructures have been demonstrated as promising carriers for gene delivery. In the carrier design, spatiotemporally programmable assembly of DNA under nanoconfinement is important but has proven highly challenging due to the complexity–scalability–error of DNA. Herein, a DNA nanotechnology-based strategy via the cascade hybridization chain reaction (HCR) of DNA hairpins in polymeric nanoframework has been developed to achieve spatiotemporally programmable assembly of DNA under nanoconfinement for precise siRNA delivery. The nanoframework is prepared via precipitation polymerization with Acrydite-DNA as cross-linker. The potential energy stored in the loops of DNA hairpins can overcome the steric effect in the nanoframework, which can help initiate cascade HCR of DNA hairpins and achieve efficient siRNA loading. The designer tethering sequence between DNA and RNA guarantees a triphosadenine triggered siRNA release specifically in cellular cytoplasm. Nanoframework provides stability and ease of functionalization, which helps address the complexity–scalability–error of DNA. It is exemplified that the phenylboronate installation on nanoframework enhanced cellular uptake and smoothed the lysosomal escape. Cellular results show that the siRNA loaded nanoframework down-regulated the levels of relevant mRNA and protein. In vivo experiments show significant therapeutic efficacy of using siPLK1 loaded nanoframework to suppress tumor growth.

Sensitive electrochemical monitoring of nucleic acids coupling DNA nanostructures with hybridization chain reaction

2013 ◽  
Vol 783 ◽  
pp. 17-23 ◽  
Author(s):  
Junyang Zhuang ◽  
Libing Fu ◽  
Mingdi Xu ◽  
Huanghao Yang ◽  
Guonan Chen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Electrochemical Monitoring

A C-HCR assembly of branched DNA nanostructures for amplified uracil-DNA glycosylase assays

2017 ◽  
Vol 53 (96) ◽  
pp. 12878-12881 ◽  
Author(s):  
Jing Wang ◽  
Min Pan ◽  
Jie Wei ◽  
Xiaoqing Liu ◽  
Fuan Wang
Keyword(s):  
Dna Glycosylase ◽  
Hybridization Chain Reaction ◽  
Selective Detection ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Uracil Dna Glycosylase ◽  
Branched Dna

The amplified and selective detection of uracil-DNA glycosylase was enabled by a two-layered cascaded hybridization chain reaction machinery.

scholarly journals DNA Origami Model for Simple Image Decoding

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Risheng Wang ◽  
Zhixiang Yin ◽  
Jianzhong Cui ◽  
Jing Yang ◽  
Zhen Tang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Molecular Beacon ◽  
Important Application ◽  
Dna Origami ◽  
Hairpin Structure ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Reaction Solution ◽  
Application Methods ◽  
Image Decoding

DNA origami is the application of self-assembly in nanotechnology. The combination of DNA origami and hybridization chain reaction is one of the important application methods of DNA origami. In this paper, DNA origami is used to design the cipher pattern on the base of origami. The cipher chain, which is put into the reaction solution, hybridizes with the molecular beacon and the hairpin structure that form the cipher pattern to build a DNA origami model that can decode the pattern. The cipher chain consists of the starting chain and the intermediate chain. When the cipher is correct, the cipher chain can open the molecular beacon and the hairpin structure to display the cipher pattern on the origami base in the solution.

Size controllable DNA nanogels from the self-assembly of DNA nanostructures through multivalent host–guest interactions

Nanoscale ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 222-230 ◽  
Author(s):  
Hari Veera Prasad Thelu ◽  
Shine K. Albert ◽  
Murali Golla ◽  
Nithiyanandan Krishnan ◽  
Divya Ram ◽  
...  
Keyword(s):  
Self Assembly ◽  
The Self ◽  
Dna Nanostructures

Design of cell permeable DNA nanogels using multivalent host–guest interactions is reported.

High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy

2008 ◽  
Vol 47 (11) ◽  
pp. 2135-2137 ◽  
Author(s):  
Barbara Saccà ◽  
Rebecca Meyer ◽  
Udo Feldkamp ◽  
Hendrik Schroeder ◽  
Christof M. Niemeyer
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Self Assembly ◽  
The Self ◽  
Dna Nanostructures ◽  
Real Time Monitoring
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