A C-HCR assembly of branched DNA nanostructures for amplified uracil-DNA glycosylase assays

2017 ◽  
Vol 53 (96) ◽  
pp. 12878-12881 ◽  
Author(s):  
Jing Wang ◽  
Min Pan ◽  
Jie Wei ◽  
Xiaoqing Liu ◽  
Fuan Wang
Keyword(s):  
Dna Glycosylase ◽  
Hybridization Chain Reaction ◽  
Selective Detection ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Uracil Dna Glycosylase ◽  
Branched Dna

The amplified and selective detection of uracil-DNA glycosylase was enabled by a two-layered cascaded hybridization chain reaction machinery.

scholarly journals Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Feng Li ◽  
Wenting Yu ◽  
Jiaojiao Zhang ◽  
Yuhang Dong ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Steric Effect ◽  
Sirna Delivery ◽  
Dna Nanotechnology ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Hairpins ◽  
In Vivo Experiments ◽  
Dna And Rna

AbstractDNA nanostructures have been demonstrated as promising carriers for gene delivery. In the carrier design, spatiotemporally programmable assembly of DNA under nanoconfinement is important but has proven highly challenging due to the complexity–scalability–error of DNA. Herein, a DNA nanotechnology-based strategy via the cascade hybridization chain reaction (HCR) of DNA hairpins in polymeric nanoframework has been developed to achieve spatiotemporally programmable assembly of DNA under nanoconfinement for precise siRNA delivery. The nanoframework is prepared via precipitation polymerization with Acrydite-DNA as cross-linker. The potential energy stored in the loops of DNA hairpins can overcome the steric effect in the nanoframework, which can help initiate cascade HCR of DNA hairpins and achieve efficient siRNA loading. The designer tethering sequence between DNA and RNA guarantees a triphosadenine triggered siRNA release specifically in cellular cytoplasm. Nanoframework provides stability and ease of functionalization, which helps address the complexity–scalability–error of DNA. It is exemplified that the phenylboronate installation on nanoframework enhanced cellular uptake and smoothed the lysosomal escape. Cellular results show that the siRNA loaded nanoframework down-regulated the levels of relevant mRNA and protein. In vivo experiments show significant therapeutic efficacy of using siPLK1 loaded nanoframework to suppress tumor growth.

Sensitive electrochemical monitoring of nucleic acids coupling DNA nanostructures with hybridization chain reaction

2013 ◽  
Vol 783 ◽  
pp. 17-23 ◽  
Author(s):  
Junyang Zhuang ◽  
Libing Fu ◽  
Mingdi Xu ◽  
Huanghao Yang ◽  
Guonan Chen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Electrochemical Monitoring

Three-dimensional DNA nanostructures for dual-color microRNA imaging in living cells via hybridization chain reaction

2020 ◽  
Vol 56 (49) ◽  
pp. 6668-6671
Author(s):  
Meng-Mei Lv ◽  
Zhan Wu ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Nanostructure ◽  
Dual Color ◽  
Dna Tetrahedron

A well-defined 3D DNA nanostructure was developed by combination of DNA tetrahedron and Y-shaped DNA, which allowed multiplexed, signal amplified fluorescent imaging of miRNAs in living cells via hybridization chain reaction.

Thermostable Branched DNA Nanostructures as Modular Primers for Polymerase Chain Reaction

2013 ◽  
Vol 52 (33) ◽  
pp. 8699-8702 ◽  
Author(s):  
Mark R. Hartman ◽  
Dayong Yang ◽  
Thua N. N. Tran ◽  
Kwang Lee ◽  
Jason S. Kahn ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Branched Dna ◽  
Polymerase Chain

Thermostable Branched DNA Nanostructures as Modular Primers for Polymerase Chain Reaction

2013 ◽  
Vol 125 (33) ◽  
pp. 8861-8864 ◽  
Author(s):  
Mark R. Hartman ◽  
Dayong Yang ◽  
Thua N. N. Tran ◽  
Kwang Lee ◽  
Jason S. Kahn ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Branched Dna ◽  
Polymerase Chain

scholarly journals Three-dimensional DNA nanostructures to improve the hyperbranched hybridization chain reaction

2019 ◽  
Vol 10 (42) ◽  
pp. 9758-9767 ◽  
Author(s):  
Jing Wang ◽  
Dong-Xia Wang ◽  
Jia-Yi Ma ◽  
Ya-Xin Wang ◽  
De-Ming Kong
Keyword(s):  
Nucleic Acid ◽  
Three Dimensional ◽  
Nucleic Acid Amplification ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Nucleic Acid Amplification Techniques ◽  
Detection Of Biomarkers

Nonenzymatic nucleic acid amplification techniques (e.g. the hybridization chain reaction, HCR) have shown promising potential for amplified detection of biomarkers.

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