Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite

2016 ◽  
Vol 8 (38) ◽  
pp. 6974-6981 ◽  
Author(s):  
Kathiresan Vijayaraj ◽  
Suck Won Hong ◽  
Sung-Ho Jin ◽  
Seung-Cheol Chang ◽  
Deog-Su Park
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Electrochemical Method ◽  
Graphite Electrode ◽  
Cost Effective ◽  
Glucose Biosensor ◽  
Pencil Graphite Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

A disposable glucose biosensor has been fabricated on the surface of a cost-effective pencil graphite electrode (PGE) by an electrochemical method, using glucose oxidase (GOx) and reduced graphene oxide (rGO).

scholarly journals Electrodeposition–Assisted Assembled Multilayer Films of Gold Nanoparticles and Glucose Oxidase onto Polypyrrole-Reduced Graphene Oxide Matrix and Their Electrocatalytic Activity toward Glucose

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 993 ◽  
Author(s):  
Baoyan Wu ◽  
Shihua Hou ◽  
Yongyong Xue ◽  
Zhan Chen
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Self Assembly ◽  
Electrocatalytic Activity ◽  
Multilayer Films ◽  
Glucose Biosensor ◽  
Future Design ◽  
Reduced Graphene

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.

Direct electrochemistry of glucose oxidase immobilized on ZrO2 nanoparticles-decorated reduced graphene oxide sheets for a glucose biosensor

RSC Advances ◽  
2014 ◽  
Vol 4 (57) ◽  
pp. 30358-30367 ◽  
Author(s):  
A. T. Ezhil Vilian ◽  
Shen-Ming Chen ◽  
M. Ajmal Ali ◽  
Fahad M. A. Al-Hemaid
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Direct Electrochemistry ◽  
Glucose Biosensor ◽  
Preparation Procedure ◽  
Zro2 Nanoparticles ◽  
Schematic Representation ◽  
Reduced Graphene ◽  
Graphene Oxide Sheets

Schematic representation of the preparation procedure of GOx–PLL/RGO–ZrO2 composite.

Amperometric Glucose Biosensor Based on AuPd Modified Reduced Graphene Oxide/Polyimide Film with Glucose Oxidase

2017 ◽  
Vol 164 (6) ◽  
pp. B285-B291 ◽  
Author(s):  
Xuan Shen ◽  
Xiaohong Xia ◽  
Yongling Du ◽  
Weichun Ye ◽  
Chunming Wang
Keyword(s):  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Polyimide Film ◽  
Glucose Biosensor ◽  
Reduced Graphene
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