Raman spectroscopy as a novel tool for fast characterization of the chemical composition of perivascular adipose tissue

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5999-6005 ◽  
Author(s):  
Krzysztof Czamara ◽  
Zuzanna Majka ◽  
Aleksandra Fus ◽  
Kamila Matjasik ◽  
Marta Z. Pacia ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Adipose Tissue ◽  
Chemical Composition ◽  
Therapeutic Potential ◽  
Adipose Tissues ◽  
Perivascular Adipose Tissue ◽  
New Targets ◽  
Lipid Unsaturation ◽  
Thoracic And Abdominal

One of the new targets of untapped therapeutic potential is perivascular adipose tissue (pVAT). Based on Raman spectroscopy we demonstrated that the lipid unsaturation degree was clearly distinct in various types of adipose tissues, in particular thoracic and abdominal pVATs, and was influenced by the age of animals.

A new approach to study human perivascular adipose tissue of the internal mammary artery by fiber-optic Raman spectroscopy supported by spectral modelling

The Analyst ◽  
2021 ◽  
Vol 146 (1) ◽  
pp. 270-276
Author(s):  
Zuzanna Majka ◽  
Krzysztof Czamara ◽  
Piotr Wegrzyn ◽  
Radoslaw Litwinowicz ◽  
Joanna Janus ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Adipose Tissue ◽  
Coronary Atherosclerosis ◽  
Internal Mammary Artery ◽  
Fiber Optic ◽  
Carotenoid Content ◽  
Perivascular Adipose Tissue ◽  
New Approach ◽  
Internal Mammary ◽  
Lipid Unsaturation

A Raman-based assessment of carotenoid content and lipid unsaturation degree in the perivascular adipose tissue may reflect its functional status in patients with advanced coronary atherosclerosis.

scholarly journals Regional Heterogeneity of Perivascular Adipose Tissue: Morphology, Origin, and Secretome

2021 ◽  
Vol 12 ◽  
Author(s):  
Xinzhi Li ◽  
Zhongyuan Ma ◽  
Yi Zhun Zhu
Keyword(s):  
Adipose Tissue ◽  
Developmental Trajectory ◽  
Low Grade ◽  
Regional Heterogeneity ◽  
Paracrine Factors ◽  
Adipose Tissues ◽  
Perivascular Adipose Tissue ◽  
Wide Range ◽  
Fat Depot ◽  
Low Grade Inflammation

Perivascular adipose tissue (PVAT) is a unique fat depot with local and systemic impacts. PVATs are anatomically, developmentally, and functionally different from classical adipose tissues and they are also different from each other. PVAT adipocytes originate from different progenitors and precursors. They can produce and secrete a wide range of autocrine and paracrine factors, many of which are vasoactive modulators. In the context of obesity-associated low-grade inflammation, these phenotypic and functional differences become more evident. In this review, we focus on the recent findings of PVAT’s heterogeneity by comparing commonly studied adipose tissues around the thoracic aorta (tPVAT), abdominal aorta (aPVAT), and mesenteric artery (mPVAT). Distinct origins and developmental trajectory of PVAT adipocyte potentially contribute to regional heterogeneity. Regional differences also exist in ways how PVAT communicates with its neighboring vasculature by producing specific adipokines, vascular tone regulators, and extracellular vesicles in a given microenvironment. These insights may inspire new therapeutic strategies targeting the PVAT.

Nanocrystalline Alloys of II-VI Semiconductors from Molecular Multi-Layer Templates

1999 ◽  
Vol 581 ◽  
Author(s):  
L. Cristofolini ◽  
P. Facci ◽  
M.P. Fontana
Keyword(s):  
Raman Spectroscopy ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Electronic Properties ◽  
Size Control ◽  
Fine Tuning ◽  
Synthesis And Characterization ◽  
Mixed Composition ◽  
Micro Raman Spectroscopy

ABSTRACTWe present the synthesis and characterization of nanocrystalline II-VI semiconductors of mixed composition (CdSe/ZnSe, CdS) grown in fatty acid Langmuir-Schaefer multi-layer templates. The controlled production of i) homogeneous nanocrystalline CdxZn1−xSe alloys and ii) heterogeneous mixtures of different pure composition II-VI semiconductors such as CdSe and ZnSe provides, in addition to the size control, the parameter of the chemical composition for the fine tuning of the electronic properties. The samples are characterized bty optical and quasi-resonance micro-Raman spectroscopy.

scholarly journals Distinct Chemical Changes in Abdominal but Not in Thoracic Aorta upon Atherosclerosis Studied Using Fiber Optic Raman Spectroscopy

2020 ◽  
Vol 21 (14) ◽  
pp. 4838 ◽  
Author(s):  
Krzysztof Czamara ◽  
Zuzanna Majka ◽  
Magdalena Sternak ◽  
Mateusz Koziol ◽  
Renata B. Kostogrys ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Composition ◽  
Thoracic Aorta ◽  
Cross Sections ◽  
Fiber Optic ◽  
Vascular Wall ◽  
Protein Ratio ◽  
Perivascular Adipose Tissue ◽  
Aorta Wall ◽  
Chemical Contents

Fiber optic Raman spectroscopy and Raman microscopy were used to investigate alterations in the aorta wall and the surrounding perivascular adipose tissue (PVAT) in the murine model of atherosclerosis (Apoe-/-/Ldlr-/- mice). Both abdominal and thoracic parts of the aorta were studied to account for the heterogenic chemical composition of aorta and its localization-dependent response in progression of atherosclerosis. The average Raman spectra obtained for both parts of aorta cross sections revealed that the chemical composition of intima-media layers along aorta remains relatively homogeneous while the lipid content in the adventitia layer markedly increases with decreasing distance to PVAT. Moreover, our results demonstrate that the increase of the lipid to protein ratio in the aorta wall correlates directly with the increased unsaturation level of lipids in PVAT and these changes occur only in the abdominal, but not in thoracic, aorta. In summary, distinct pathophysiological response in the aortic vascular wall could be uncovered by fiber optic Raman spectroscopy based on simple parameters detecting chemical contents of lipids in PVAT.

scholarly journals Perivascular Adipose Tissue Regulates Vascular Function by Targeting Vascular Smooth Muscle Cells

2020 ◽  
Vol 40 (5) ◽  
pp. 1094-1109 ◽  
Author(s):  
Lin Chang ◽  
Minerva T. Garcia-Barrio ◽  
Y. Eugene Chen
Keyword(s):  
Adipose Tissue ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Vascular Function ◽  
Muscle Cells ◽  
The Body ◽  
Adipose Tissues ◽  
Perivascular Adipose Tissue

Adipose tissues are present at multiple locations in the body. Most blood vessels are surrounded with adipose tissue which is referred to as perivascular adipose tissue (PVAT). Similarly to adipose tissues at other locations, PVAT harbors many types of cells which produce and secrete adipokines and other undetermined factors which locally modulate PVAT metabolism and vascular function. Uncoupling protein-1, which is considered as a brown fat marker, is also expressed in PVAT of rodents and humans. Thus, compared with other adipose tissues in the visceral area, PVAT displays brown-like characteristics. PVAT shows a distinct function in the cardiovascular system compared with adipose tissues in other depots which are not adjacent to the vascular tree. Growing and extensive studies have demonstrated that presence of normal PVAT is required to maintain the vasculature in a functional status. However, excessive accumulation of dysfunctional PVAT leads to vascular disorders, partially through alteration of its secretome which, in turn, affects vascular smooth muscle cells and endothelial cells. In this review, we highlight the cross talk between PVAT and vascular smooth muscle cells and its roles in vascular remodeling and blood pressure regulation.

scholarly journals Detection, counting and characterization of nanoplastics in marine bioindicators: a proof of principle study

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Andrea Valsesia ◽  
Jeremie Parot ◽  
Jessica Ponti ◽  
Dora Mehn ◽  
Rita Marino ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Composition ◽  
Food Chain ◽  
Limit Of Detection ◽  
Polystyrene Spheres ◽  
Biological Matrices ◽  
Regulatory Bodies ◽  
Controlled Exposure ◽  
Proof Of Principle

AbstractPlastic particulates in the environment pose an increasing concern for regulatory bodies due to their potential risk to higher organisms (including humans) as they enter the food chain. Nanoplastics (defined here as smaller than 1 μm) are particularly challenging to detect and analyze at environmentally relevant concentrations and in biological matrices. The tunicate Ciona Robusta is an effective bioindicator for microplastics and nanoplastic contamination in the marine environment, due to its capacity to filter substantial volumes of water and to accumulate particulates. In this proof-of-principle study that demonstrates a complete methodology, following controlled exposure using spiked samples of a model nanoplastic (100 nm diameter polystyrene spheres) the nanoparticles were separated from an enzymatically digested biological matrix, purified and concentrated for analysis. The described method yields an approximate value for nanoplastic concentration in the organism (with a limit of detection of 106 particles/organism, corresponding to 1 ng/g) and provides the chemical composition by Raman spectroscopy. Furthermore, this method can be extended to other biological matrices and used to quantitatively monitor the accumulation of nanoplastics in the environment and food chain.

Sign in / Sign up

Export Citation Format

Share Document