Ratiometric red-emission fluorescence detection of Al3+in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission

The Analyst ◽  
2018 ◽  
Vol 143 (21) ◽  
pp. 5285-5294 ◽  
Author(s):  
Lok Nath Neupane ◽  
Pramod Kumar Mehta ◽  
Semin Oh ◽  
See-Hyoung Park ◽  
Keun-Hyeung Lee
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Fluorescence Detection ◽  
Live Cells ◽  
Red Emission ◽  
Aggregation Induced Emission ◽  
Ratiometric Detection ◽  
Pure Aqueous Solution

A novel peptidyl probe using aggregation induced emission was proposed for the selective ratiometric detection of Al3+ions in pure aqueous solutions and live cells.

Cellular membrane-anchored fluorescent probe with aggregation-induced emission characteristics for selective detection of Cu2+ ions

2017 ◽  
Vol 196 ◽  
pp. 377-393 ◽  
Author(s):  
Danni Liu ◽  
Shenglu Ji ◽  
Heran Li ◽  
Liang Hong ◽  
Deling Kong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Membrane ◽  
Water Solubility ◽  
Live Cells ◽  
Probe Design ◽  
Specific Cell ◽  
Selective Detection ◽  
High Concentration ◽  
Aggregation Induced Emission ◽  
Emissive Probes

The exploration of advanced fluorescent probes that can detect divalent copper (Cu2+) in aqueous environments and even in live organisms is particularly valuable for understanding the occurrence and development of Cu2+-related diseases. In this work, we report the design and synthesis of an aggregation-induced emission luminogen (AIEgen)-based probe (TPE-Py-EEGTIGYG) by integrating an AIEgen, TPE-Py, with a peptide, EEGTIGYG, which can selectively detect Cu2+ in both aqueous solution and live cells. Peptide EEGTIGYG has dual functionality in the probe design, namely improving water solubility and providing specific cell membrane-binding ability. TPE-Py-EEGTIGYG can self-assemble into nanoaggregates at high concentration in aqueous solution (e.g., 25 μM), which possess large fluorescence output due to the restriction of intramolecular rotation of the phenyl rings on TPE-Py. The fluorescence of the TPE-Py-EEGTIGYG nanoaggregates can be significantly quenched by Cu2+ but not by other metal ions, achieving the selective detection of Cu2+ in aqueous media. Furthermore, TPE-Py-EEGTIGYG can exist as a molecular species and is very weakly fluorescent in dilute aqueous solution (e.g., 5 μM), but can however largely switch on its fluorescence upon specifically anchoring onto the cell membrane. The emissive probes on the cell membrane can be used for the detection of Cu2+ ions that move in and out of cells with a fluorescence “turn-off” mode.

scholarly journals Interactions of Mycotoxin Alternariol with Cyclodextrins and its Removal from Aqueous Solution by Beta-Cyclodextrin Bead Polymer

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 428 ◽  
Author(s):  
Fliszár-Nyúl ◽  
Lemli ◽  
Kunsági-Máté ◽  
Szente ◽  
Poór
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Fluorescence Detection ◽  
Quaternary Ammonium ◽  
Chronic Exposure ◽  
Binding Ability ◽  
Promising Tool ◽  
Beta Cyclodextrin ◽  
Modeling Studies

Alternariol is an Alternaria mycotoxin that appears in fruits, tomatoes, oilseeds, and corresponding products. Chronic exposure to it can induce carcinogenic and xenoestrogenic effects. Cyclodextrins (CDs) are ring-shaped molecules built up by glucose units, which form host–guest type complexes with some mycotoxins. Furthermore, insoluble CD polymers seem suitable for the extraction/removal of mycotoxins from aqueous solutions. In this study, the interactions of alternariol with β- and γ-CDs were tested by employing fluorescence spectroscopic and modeling studies. Moreover, the removal of alternariol from aqueous solutions by insoluble β-CD bead polymer (BBP) was examined. Our major observations/conclusions are the following: (1) CDs strongly increased the fluorescence of alternariol, the strongest enhancement was induced by the native γ-CD at pH 7.4. (2) Alternariol formed the most stable complexes with the native γ-CD (logK = 3.2) and the quaternary ammonium derivatives (logK = 3.4–3.6) at acidic/physiological pH and at pH 10.0, respectively. (3) BBP effectively removed alternariol from aqueous solution. (4) The alternariol-binding ability of β-CD polymers was significantly higher than was expected based on their β-CD content. (5) CD technology seems a promising tool to improve the fluorescence detection of alternariol and/or to develop new mycotoxin binders to decrease alternariol exposure.

Ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo achieved by using an aggregation induced emission (AIE)-based nanoprobe

2016 ◽  
Vol 52 (45) ◽  
pp. 7288-7291 ◽  
Author(s):  
Yong Huang ◽  
Peisheng Zhang ◽  
Meng Gao ◽  
Fang Zeng ◽  
Anjun Qin ◽  
...  
Keyword(s):  
Live Cells ◽  
Aggregation Induced Emission ◽  
Fluorescent Nanoprobe ◽  
Ratiometric Detection

An AIE-based fluorescent nanoprobe was prepared for ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo.

Selective red-emission detection for mercuric ions in aqueous solution and cells using a fluorescent probe based on an unnatural peptide receptor

2019 ◽  
Vol 17 (14) ◽  
pp. 3590-3598 ◽  
Author(s):  
Lok Nath Neupane ◽  
Pramod Kumar Mehta ◽  
Joon-Uk Kwon ◽  
See-Hyoung Park ◽  
Keun-Hyeung Lee
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Live Cells ◽  
Red Emission ◽  
Peptide Receptor ◽  
Significant Challenge ◽  
Mercuric Ions ◽  
Emission Detection

The selective ratiometric red-emission detection of Hg2+ions in aqueous buffered solutions and live cells is still a significant challenge.

Selective ratiometric red-emission detection of In3+ in aqueous solutions and in live cells using a fluorescent peptidyl probe and metal chelating agent

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 4031-4040
Author(s):  
Joohee Park ◽  
Hyeongseok Yu ◽  
See-Hyoung Park ◽  
Keun-Hyeung Lee
Keyword(s):  
Aqueous Solutions ◽  
Ground Water ◽  
Detection Method ◽  
Chelating Agent ◽  
Fluorescent Detection ◽  
Live Cells ◽  
Red Emission ◽  
Metal Chelating ◽  
Emission Detection

A ratiometric fluorescent detection method for In3+ in aqueous buffered solution, ground water, and live cells was developed.

Effect of thermal history of aqueous solutions of KCl on the metastable zone width

1984 ◽  
Vol 49 (3) ◽  
pp. 559-569 ◽  
Author(s):  
Jaroslav Nývlt
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Thermal History ◽  
Equilibrium Solubility ◽  
Metastable Zone Width ◽  
Zone Width ◽  
Preceding Experiment ◽  
History Of ◽  
Metastable Zone ◽  
The Given

The metastable zone width of an aqueous solution of KCI was measured as a function of the time and temperature of overheating above the equilibrium solubility temperature. It has been found that when the experiments follow close upon one another, the parameters of the preceding experiment affect the results of the experiment to follow.The results are interpreted in terms of hypotheses advanced in the literature to account for the effect of thermal history of solution. The plausibility and applicability of these hypotheses are assessed for the given cause of aqueous solution of a well soluble electrolyte.

scholarly journals I. Thermo-electric behaviour of aqueous solutions with mercurial electrodes

1879 ◽  
Vol 29 (196-199) ◽  
pp. 472-482 ◽  
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Internal Diameter ◽  
Thermo Electric ◽  
Thin Glass ◽  
Mercury Surface

In order to investigate this subject, I devised and constructed the following apparatus :—A and B are two thin glass basins, 81 millims. internal diameter (= 5,153 sq. millims. of mercury surface), and 6·0 centims. deep; each containing a layer of mercury about 1·0 centim. deep, covered by a layer, about 3 centims. deep, of the aqueous solution to be examined.

scholarly journals Meta-studtite stability in aqueous solutions. Impact of HCO3−, H2O2 and ionizing radiation on dissolution and speciation

2021 ◽  
Author(s):  
Junyi Li ◽  
Zoltán Szabó ◽  
Mats Jonsson
Keyword(s):  
Aqueous Solution ◽  
Ionizing Radiation ◽  
Aqueous Solutions ◽  
Carbonate Complexes ◽  
C Nmr

Four different uranyl-(peroxide)-carbonate complexes were identified during studtite and meta-studtite dissolution in aqueous solution containing 10 mM HCO3− by 13C NMR.

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