Ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo achieved by using an aggregation induced emission (AIE)-based nanoprobe

2016 ◽  
Vol 52 (45) ◽  
pp. 7288-7291 ◽  
Author(s):  
Yong Huang ◽  
Peisheng Zhang ◽  
Meng Gao ◽  
Fang Zeng ◽  
Anjun Qin ◽  
...  
Keyword(s):  
Live Cells ◽  
Aggregation Induced Emission ◽  
Fluorescent Nanoprobe ◽  
Ratiometric Detection

An AIE-based fluorescent nanoprobe was prepared for ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo.

Ratiometric red-emission fluorescence detection of Al3+in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission

The Analyst ◽  
2018 ◽  
Vol 143 (21) ◽  
pp. 5285-5294 ◽  
Author(s):  
Lok Nath Neupane ◽  
Pramod Kumar Mehta ◽  
Semin Oh ◽  
See-Hyoung Park ◽  
Keun-Hyeung Lee
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Fluorescence Detection ◽  
Live Cells ◽  
Red Emission ◽  
Aggregation Induced Emission ◽  
Ratiometric Detection ◽  
Pure Aqueous Solution

A novel peptidyl probe using aggregation induced emission was proposed for the selective ratiometric detection of Al3+ions in pure aqueous solutions and live cells.

A highly selective fluorescent nanoprobe based on AIE and ESIPT for imaging hydrogen sulfide in live cells and zebrafish

2017 ◽  
Vol 1 (5) ◽  
pp. 838-845 ◽  
Author(s):  
Peisheng Zhang ◽  
Xuezheng Nie ◽  
Meng Gao ◽  
Fang Zeng ◽  
Anjun Qin ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Live Cells ◽  
Specific Detection ◽  
Fluorescent Nanoprobe

An AIE/ESIPT-based nanoprobe has been developed for the specific detection and imaging of H2Sin vitroandin vivo.

Activatable Fluorescent Nanoprobe with Aggregation-Induced Emission Characteristics for Selective In Vivo Imaging of Elevated Peroxynitrite Generation

2016 ◽  
Vol 28 (33) ◽  
pp. 7249-7256 ◽  
Author(s):  
Zhegang Song ◽  
Duo Mao ◽  
Simon H. P. Sung ◽  
Ryan T. K. Kwok ◽  
Jacky W. Y. Lam ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Emission Characteristics ◽  
Aggregation Induced Emission ◽  
Fluorescent Nanoprobe

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Sensitive and specific detection of peroxynitrite and in-vivo imaging of inflammation by a "simple" AIE bioprobe

2021 ◽  
Author(s):  
Huilin Xie ◽  
Jingtian Zhang ◽  
Chao Chen ◽  
Feiyi Sun ◽  
Haixiang Liu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescence Enhancement ◽  
Specific Detection ◽  
Aggregation Induced Emission

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO−) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage...

scholarly journals A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Soudeh Moghadasi ◽  
Marischa Elveny ◽  
Heshu Sulaiman Rahman ◽  
Wanich Suksatan ◽  
Abduladheem Turki Jalil ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Ethical Issues ◽  
Therapeutic Potential ◽  
Kidney Diseases ◽  
Experimental Models ◽  
Live Cells ◽  
Target Cells ◽  
Intercellular Interaction ◽  
Micro Rnas

AbstractRecently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs are not related to their differentiation potentials, but rather are exerted through the release of soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, and owning targeting capability and organotropism, exosomes are considered nanocarriers for their possible use in diagnosis and therapy. Exosomes convey functional molecules such as long non-coding RNAs (lncRNAs) and micro-RNAs (miRNAs), proteins (e.g., chemokine and cytokine), and lipids from MSCs to the target cells. They participate in intercellular interaction procedures and enable the repair of damaged or diseased tissues and organs. Findings have evidenced that exosomes alone are liable for the beneficial influences of MSCs in a myriad of experimental models, suggesting that MSC- exosomes can be utilized to establish a novel cell-free therapeutic strategy for the treatment of varied human disorders, encompassing myocardial infarction (MI), CNS-related disorders, musculoskeletal disorders (e.g. arthritis), kidney diseases, liver diseases, lung diseases, as well as cutaneous wounds. Importantly, compared with MSCs, MSC- exosomes serve more steady entities and reduced safety risks concerning the injection of live cells, such as microvasculature occlusion risk. In the current review, we will discuss the therapeutic potential of MSC- exosomes as an innovative approach in the context of regenerative medicine and highlight the recent knowledge on MSC- exosomes in translational medicine, focusing on in vivo researches.

scholarly journals Insights in Behavior of Variably Formulated Alginate-Based Microcapsules for Cell Transplantation

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Pia Montanucci ◽  
Silvia Terenzi ◽  
Claudio Santi ◽  
Ilaria Pennoni ◽  
Vittorio Bini ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Chemical Properties ◽  
Live Cells ◽  
High Performing ◽  
Physical Chemical ◽  
Degenerative Disorders ◽  
Selection Of ◽  
Better Than

Alginate-based microencapsulation of live cells may offer the opportunity to treat chronic and degenerative disorders. So far, a thorough assessment of physical-chemical behavior of alginate-based microbeads remains cloudy. A disputed issue is which divalent cation to choose for a high performing alginate gelling process. Having selected, in our system, high mannuronic (M) enriched alginates, we studied different gelling cations and their combinations to determine their eventual influence on physical-chemical properties of the final microcapsules preparation,in vitroandin vivo. We have shown that used of ultrapure alginate allows for high biocompatibility of the formed microcapsules, regardless of gelation agents, while use of different gelling cations is associated with corresponding variable effects on the capsules’ basic architecture, as originally reported in this work. However, only the final application which the capsules are destined to will ultimately guide the selection of the ideal, specific gelling divalent cations, since in principle there are no capsules that are better than others.

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