scholarly journals Enhanced antimicrobial effect of berberine in nanogel carriers with cationic surface functionality

2017 ◽  
Vol 5 (38) ◽  
pp. 7885-7897 ◽  
Author(s):  
Mohammed J. Al-Awady ◽  
Adelaide Fauchet ◽  
Gillian M. Greenway ◽  
Vesselin N. Paunov
Keyword(s):  
Cell Wall ◽  
Antimicrobial Effect ◽  
Cationic Polyelectrolyte ◽  
Surface Functionality ◽  
Strong Enhancement ◽  
Antifungal Action ◽  
Electrostatic Adhesion

We demonstrate a strong enhancement in the antimicrobial and antifungal action of berberine encapsulated in nanogel carriers whose surface is functionalised with a cationic polyelectrolyte. The effect comes from the electrostatic adhesion of the cationic nanocarriers on the cell wall.

scholarly journals Dual-functionalised shellac nanocarriers give a super-boost of the antimicrobial action of berberine

2019 ◽  
Vol 1 (2) ◽  
pp. 858-872 ◽  
Author(s):  
Saba S. M. Al-Obaidy ◽  
Gillian M. Greenway ◽  
Vesselin N. Paunov
Keyword(s):  
Cell Walls ◽  
Local Delivery ◽  
Microbial Cell ◽  
Antimicrobial Action ◽  
Surface Functionality ◽  
Strong Enhancement ◽  
Electrostatic Adhesion

We demonstrate a strong enhancement of the antimicrobial action of berberine encapsulated in shellac nanoparticles with dual surface functionality. This is attributed to the electrostatic adhesion of the cationic nanocarriers to the microbial cell walls which leads to their accumulation and local delivery of berberine.

scholarly journals Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans

2021 ◽  
Vol 8 ◽  
Author(s):  
Thomas Voit ◽  
Fabian Cieplik ◽  
Johannes Regensburger ◽  
Karl-Anton Hiller ◽  
Anita Gollmer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Fungal Infections ◽  
Cell Envelope ◽  
Antimicrobial Photodynamic Therapy ◽  
Fungal Cell ◽  
Before And After ◽  
Antifungal Action ◽  
Complete Cell ◽  
Further Development

The antimicrobial photodynamic therapy (aPDT) is a promising approach for the control of microbial and especially fungal infections such as mucosal mycosis. TMPyP [5,10,15, 20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate] is an effective photosensitizer (PS) that is commonly used in aPDT. The aim of this study was to examine the localization of TMPyP in Candida albicans before and after irradiation with visible light to get information about the cellular mechanism of antifungal action of the photodynamic process using this PS. Immediately after incubation of C. albicans with TMPyP, fluorescence microscopy revealed an accumulation of the PS in the cell envelope. After irradiation with blue light the complete cell showed red fluorescence, which indicates, that aPDT is leading to a damage in the cell wall with following influx of PS into the cytosol. Incubation of C. albicans with Wheat Germ Agglutinin (WGA) could confirm the cell wall as primary binding site of TMPyP. The finding that the porphyrin accumulates in the fungal cell wall and does not enter the interior of the cell before irradiation makes it unlikely that resistances can emerge upon aPDT. The results of this study may help in further development and modification of PS in order to increase efficacy against fungal infections such as those caused by C. albicans.

scholarly journals Structure, Cellular Distribution, Antigenicity, and Biological Functions of Fonsecaea pedrosoi Ceramide Monohexosides

2005 ◽  
Vol 73 (12) ◽  
pp. 7860-7868 ◽  
Author(s):  
Leonardo Nimrichter ◽  
Mariana D. Cerqueira ◽  
Eduardo A. Leitão ◽  
Kildare Miranda ◽  
Ernesto S. Nakayasu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Difference ◽  
Structural Diversity ◽  
Fungal Species ◽  
Hydroxyl Group ◽  
Current Evidence ◽  
Fonsecaea Pedrosoi ◽  
Fungal Cell ◽  
Long Chain Base ◽  
Antifungal Action

ABSTRACT Monohexosylceramides (CMHs, or cerebrosides) have been reported as membrane and cell wall constituents of both pathogenic and nonpathogenic fungi, presenting remarkable differences in their ceramide moiety compared to mammalian CMHs. Current evidence suggests that CMHs are involved in fungal differentiation and growth and contribute to host immune response. Here we describe a structural diversity between cerebrosides obtained from different forms of the human pathogen Fonsecaea pedrosoi. The major CMH species produced by conidial forms displayed the same structure previously demonstrated by our group for mycelia, an N-2′-hydroxyhexadecanoyl-1-β-d-glucopyranosyl-9-methyl-4,8-sphingadienine. However, the major cerebroside species purified from sclerotic cells carries an additional hydroxyl group, bound to its long-chain base. The structural difference between cerebrosides from mycelial and sclerotic cells was apparently not relevant for their antigenicity, since they were both recognized at similar levels by sera from individuals with chromoblastomycosis and a monoclonal antibody to a conserved cerebroside structure. Preincubation of fungal cells with anti-CMH monoclonal antibodies had no effect on the interaction of F. pedrosoi sclerotic cells with murine macrophages. In contrast to what has been described for other fungal species, sclerotic bodies are resistant to the antifungal action of anti-CMH antibodies. Immunofluorescence analysis showed that recognition of sclerotic cells by these antibodies only occurs at cell wall regions in which melanization is not evident. Accordingly, melanin removal with alkali results in an increased reaction of fungal cells with anti-CMH antibodies. Our results indicate that cerebroside expression in F. pedrosoi cells is associated with dimorphism and melanin assembly on the fungal cell wall.

scholarly journals The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5463
Author(s):  
Monika Staniszewska ◽  
Łukasz Kuryk ◽  
Aleksander Gryciuk ◽  
Joanna Kawalec ◽  
Marta Rogalska ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Wall ◽  
Cell Membrane ◽  
Chitinolytic Activity ◽  
Irreversible Damage ◽  
Fungal Cell ◽  
Action Mode ◽  
Structure Activity ◽  
Dependent Inhibition ◽  
Antifungal Action

Our study aimed to characterise the action mode of N-phenacyldibromobenzimidazoles against C. albicans and C. neoformans. Firstly, we selected the non-cytotoxic most active benzimidazoles based on the structure–activity relationships showing that the group of 5,6-dibromobenzimidazole derivatives are less active against C. albicans vs. 4,6-dibromobenzimidazole analogues (5e–f and 5h). The substitution of chlorine atoms to the benzene ring of the N-phenacyl substituent extended the anti-C. albicans action (5e with 2,4-Cl2 or 5f with 3,4-Cl2). The excellent results for N-phenacyldibromobenzimidazole 5h against the C. albicans reference and clinical isolate showed IC50 = 8 µg/mL and %I = 100 ± 3, respectively. Compound 5h was fungicidal against the C. neoformans isolate. Compound 5h at 160–4 µg/mL caused irreversible damage of the fungal cell membrane and accidental cell death (ACD). We reported on chitinolytic activity of 5h, in accordance with the patterns observed for the following substrates: 4-nitrophenyl-N-acetyl-β-d-glucosaminide and 4-nitrophenyl-β-d-N,N′,N″-triacetylchitothiose. Derivative 5h at 16 µg/mL: (1) it affected cell wall by inducing β-d-glucanase, (2) it caused morphological distortions and (3) osmotic instability in the C. albicans biofilm-treated. Compound 5h exerted Candida-dependent inhibition of virulence factors.

scholarly journals Boosting the antimicrobial action of vancomycin formulated in shellac nanoparticles of dual-surface functionality

2019 ◽  
Vol 7 (19) ◽  
pp. 3119-3133 ◽  
Author(s):  
Saba S. M. Al-Obaidy ◽  
Ahmed F. Halbus ◽  
Gillian M. Greenway ◽  
Vesselin N. Paunov
Keyword(s):  
Cell Membranes ◽  
Microbial Cell ◽  
Antimicrobial Action ◽  
Surface Functionality ◽  
Strong Enhancement

We demonstrate a strong enhancement of the antimicrobial action of vancomycin encapsulated in shellac nanocarriers with cationic surface functionality which concentrate on the microbial cell membranes.

scholarly journals Comparative Antimicrobial Effect of Boswellia, Lichen, Coffea peel, Asafoetida, Saussurea Costus, Rhatany, Anise and Cumin Extracts on Some Animal Pathogens

2020 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Rehab Mohamed Atta El-Desoukey
Keyword(s):  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Antimicrobial Compound ◽  
Cuminum Cyminum ◽  
Pimpinella Anisum ◽  
Animal Pathogens ◽  
Antifungal Action ◽  
Bacteria And Fungi ◽  
Agar Well Diffusion Method

Numerous plants have so far been utilized for the treatment and the executives of different illnesses since the start of human development. One of the regular issues in the clinical world, spreading of bacterial opposition against anti-infection agents, so one of the most significant strides in microbiological investigates is to locate another antimicrobial compound with insignificant reactions. Because of the nearness of organic dynamic mixes in plant and herbs and its utilization in conventional Objective medication and sustenance, it appears that this plants and herbs contain limit significant antimicrobial. So the aim of this study is to explore the antimicrobial action of Boswellia, Lichen, Coffea peel, Rhatany (Krameria triandra), Cuminum cyminum, Pimpinella anisum, Asafoetida, and Saussurea costus aqueous and solvent extracts on some therapeutically significant bacteria and fungi isolated from animals. Hot and cold aqueous extracts in addition to alcohol extracts of Boswellia, Lichen, Coffea peel, Rhatany (Krameria triandra), Cuminum cyminum, Pimpinella anisum , Asafoetida,and Saussurea costus were assessed for their antimicrobial effect against Streptococcus, Escherichia coli Salmonella, klebsiella and Candida albicans by agar well diffusion method. Every single examined plants and herbs indicated antibacterial impact against examined pathogenic microscopic organisms particularly Rhatany, Asafoetida, Lichen, and Pimpinella anisum. The lesser impact was for Coffea peel. Also the most antifungal action against Candida albican swas for Rhatany. For the most part the viable concentrate was the cold aqueous extract for all analyzed plants and herbs.

Antifungal action of Carica papaya latex: Isolation of fungal cell wall hydrolysing enzymes

Mycoses ◽  
2009 ◽  
Vol 34 (11-12) ◽  
pp. 469-477 ◽  
Author(s):  
R. Giordani ◽  
M. Siepaio ◽  
J. Moulin-Traffort ◽  
P. Régli
Keyword(s):  
Cell Wall ◽  
Carica Papaya ◽  
Fungal Cell Wall ◽  
Fungal Cell ◽  
Antifungal Action ◽  
Papaya Latex ◽  
Carica Papaya Latex

scholarly journals ESSENTIAL OILS, A POSSIBLE SOLUTION TO OVERCOME ANTIMICROBIAL RESISTANCE CRISIS

2015 ◽  
Vol 62 (3) ◽  
pp. 280-283
Author(s):  
Adrian-Vasile Craciun ◽  
◽  
Daniela Chiru ◽  
Otilia Marginean ◽  
◽  
...  
Keyword(s):  
Cell Wall ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Action Spectrum ◽  
Current Medical ◽  
Ideal Solution ◽  
Pathogenic Microorganisms ◽  
Current Medical Practice ◽  
Antifungal Action ◽  
Defensive Mechanisms

Having emerged as an ideal solution against pathogenic microorganisms, antimicrobial medication seems to have lost the battle today. The ability of the living world to survive and adapt exceeds the synthetic products power of action, leading us today to a real crisis of microbial resistance to antibiotics. An unexpected solution may come also from the bosom of nature, by reconsidering the role and place of the essential oils as antimicrobial combat weapons. Defensive mechanisms created by nature, the essential oils demonstrate extraordinary antibacterial, antiviral and antifungal activity. They also help the synthetic antibiotics in their battle. Among the essential oils and the antibiotics, often intervene synergistic or additive interactions. The essential oils favor the antibiotics to penetrate through the cell wall and to extend their action spectrum. Undoubtedly, essential oils can be of crucial help in the current medical practice, through their complex antibacterial, antiviral and antifungal action.

scholarly journals Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers

2018 ◽  
Vol 2 (11) ◽  
pp. 2032-2044 ◽  
Author(s):  
Mohammed J. Al-Awady ◽  
Paul J. Weldrick ◽  
Matthew J. Hardman ◽  
Gillian M. Greenway ◽  
Vesselin N. Paunov
Keyword(s):  
Cell Membrane ◽  
Cell Walls ◽  
Local Delivery ◽  
Microbial Cell ◽  
Antimicrobial Action ◽  
Strong Enhancement ◽  
Acrylate Copolymer ◽  
Electrostatic Adhesion

We demonstrate a strong enhancement of the antimicrobial action of chlorhexidine which is attributed to the electrostatic adhesion of the cationic nanocarriers on the microbial cell walls which leads to their accumulation and local delivery of chlorhexidine directly on to the microbial cell membrane.

scholarly journals Breathing new life into old antibiotics: overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality

Nanoscale ◽  
2019 ◽  
Vol 11 (21) ◽  
pp. 10472-10485 ◽  
Author(s):  
Paul J. Weldrick ◽  
Sammi Iveson ◽  
Matthew J. Hardman ◽  
Vesselin N. Paunov
Keyword(s):  
Antibiotic Resistance ◽  
Cationic Polyelectrolyte ◽  
Antibacterial Resistance ◽  
Surface Functionality

We demonstrate that nanogel carriers for tetracycline and lincomycin coated with a biocompatible cationic polyelectrolyte can overcome the antibiotic resistance against a range of wound isolated pathogens.

Sign in / Sign up

Export Citation Format

Share Document