scholarly journals Highly sensitive and selective fluorescence detection of Hg2+ based on turn-on aptamer DNA silver nanoclusters

RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56289-56295 ◽  
Author(s):  
Baozhu Zhang ◽  
Chunying Wei
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Detection ◽  
Silver Nanoclusters ◽  
Fluorescent Biosensor ◽  
Turn On ◽  
Highly Sensitive ◽  
Ag Ncs

A novel turn-on fluorescent biosensor based on C–Hg2+-aptamer-1-DNA-templated silver nanoclusters (Ag NCs) was developed for the quantitative analysis of Hg2+.

scholarly journals Nucleic acid probe based on DNA-templated silver nanoclusters for turn-on fluorescence detection of tumor suppressor gene p53

RSC Advances ◽  
2018 ◽  
Vol 8 (45) ◽  
pp. 25611-25616 ◽  
Author(s):  
Dan Han ◽  
Chunying Wei
Keyword(s):  
Nucleic Acid ◽  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Fluorescence Detection ◽  
P53 Gene ◽  
Suppressor Gene ◽  
Silver Nanoclusters ◽  
Turn On ◽  
Ag Ncs ◽  
Tumor Suppressor Gene P53

“Turn-on” fluorescence detection for p53 gene based on target-triggered opening of hairpin DNA probe and synthesis of DNA-Ag NCs.

scholarly journals An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

RSC Advances ◽  
2020 ◽  
Vol 10 (58) ◽  
pp. 35374-35380
Author(s):  
Baozhu Zhang ◽  
Chunying Wei
Keyword(s):  
Target Recognition ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Ag Nanoclusters ◽  
Ag Ncs

A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs).

scholarly journals A Turn-On Detection of DNA Sequences by Means of Fluorescence of DNA-Templated Silver Nanoclusters via Unique Interactions of a Hydrated Ionic Liquid

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2889 ◽  
Author(s):  
Ye Teng ◽  
Hisae Tateishi-Karimata ◽  
Takaaki Tsuruoka ◽  
Naoki Sugimoto
Keyword(s):  
Ionic Liquid ◽  
Dna Sequences ◽  
Fluorescence Emission ◽  
Silver Nanoclusters ◽  
Dihydrogen Phosphate ◽  
Target Sequence ◽  
Aqueous Buffer ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Ag Ncs

Nucleic acid stability and structure, which are crucial to the properties of fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs), significantly change in ionic liquids. In this work, our purpose was to study DNA-Ag NCs in a buffer containing the hydrated ionic liquid of choline dihydrogen phosphate (choline dhp) to improve fluorescence for application in DNA detection. Due to the stabilisation of an i-motif structure by the choline cation, a unique fluorescence emission—that was not seen in an aqueous buffer—was observed in choline dhp and remained stable for more than 30 days. A DNA-Ag NCs probe was designed to have greater fluorescence intensity in choline dhp in the presence of a target DNA. A turn-on sensing platform in choline dhp was built for the detection of the BRCA1 gene, which is related to familial breast and ovarian cancers. This platform showed better sensitivity and selectivity in distinguishing a target sequence from a mutant sequence in choline dhp than in the aqueous buffer. Our study provides new evidence regarding the effects of structure on properties of fluorescent DNA-Ag NCs and expands the applications of fluorescent DNA-Ag NCs in an ionic liquid because of improved sensitivity and selectivity.

Highly sensitive fluorescence detection of tobacco mosaic virus RNA based on disodium 4,4′-diazidostilbene-2,2′-disulfonate tetrahydrate in situ reaction

2021 ◽  
Author(s):  
Wenbin Wang ◽  
Peipei Li ◽  
Zhixian Zheng ◽  
Di Cheng ◽  
Chengming Dong ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Fluorescence Detection ◽  
In Situ Reaction ◽  
Turn On ◽  
Reducing Ability ◽  
Virus Rna ◽  
Highly Sensitive

A novel fluorescent turn-on method for TMV RNA via the reducing ability of an AA and DES in situ reaction was reported.

A turn-on fluorescent probe for phytic acid based on ferric ion-modulated glutathione-capped silver nanoclusters

2016 ◽  
Vol 8 (34) ◽  
pp. 6382-6387 ◽  
Author(s):  
Q. Chen ◽  
M. Li ◽  
F. Zhang ◽  
R. Li ◽  
G. Chen ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Phytic Acid ◽  
Fluorescence Detection ◽  
Silver Nanoclusters ◽  
Ferric Ion ◽  
Real Samples ◽  
Turn On ◽  
Detection Assay ◽  
High Detection

A sensitive fluorescence detection assay for phytic acid (PA) in real samples was developed based on Fe3+-modulated GSH@AgNCs, with high detection selectivity, repeatability, and stability.

A new label-free and turn-on fluorescence probe for hydrogen peroxide and glucose detection based on DNA–silver nanoclusters

2015 ◽  
Vol 7 (19) ◽  
pp. 7989-7994 ◽  
Author(s):  
Hui-Xia Han ◽  
Xue Tian ◽  
Xiang-Juan Kong ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Sequences ◽  
Fluorescence Enhancement ◽  
Fluorescence Probe ◽  
Silver Nanoclusters ◽  
Enhancement Effect ◽  
Glucose Detection ◽  
Label Free ◽  
Turn On ◽  
Ag Ncs

A label-free and turn-on strategy for H2O2 and glucose detection based on the cleavage of ssDNA by ˙OH and the fluorescence enhancement effect when guanine-rich (G-rich) DNA sequences are in proximity to DNA–silver nanoclusters (DNA–Ag NCs).

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