A new label-free and turn-on fluorescence probe for hydrogen peroxide and glucose detection based on DNA–silver nanoclusters

2015 ◽  
Vol 7 (19) ◽  
pp. 7989-7994 ◽  
Author(s):  
Hui-Xia Han ◽  
Xue Tian ◽  
Xiang-Juan Kong ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Sequences ◽  
Fluorescence Enhancement ◽  
Fluorescence Probe ◽  
Silver Nanoclusters ◽  
Enhancement Effect ◽  
Glucose Detection ◽  
Label Free ◽  
Turn On ◽  
Ag Ncs

A label-free and turn-on strategy for H2O2 and glucose detection based on the cleavage of ssDNA by ˙OH and the fluorescence enhancement effect when guanine-rich (G-rich) DNA sequences are in proximity to DNA–silver nanoclusters (DNA–Ag NCs).

scholarly journals A Turn-On Detection of DNA Sequences by Means of Fluorescence of DNA-Templated Silver Nanoclusters via Unique Interactions of a Hydrated Ionic Liquid

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2889 ◽  
Author(s):  
Ye Teng ◽  
Hisae Tateishi-Karimata ◽  
Takaaki Tsuruoka ◽  
Naoki Sugimoto
Keyword(s):  
Ionic Liquid ◽  
Dna Sequences ◽  
Fluorescence Emission ◽  
Silver Nanoclusters ◽  
Dihydrogen Phosphate ◽  
Target Sequence ◽  
Aqueous Buffer ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Ag Ncs

Nucleic acid stability and structure, which are crucial to the properties of fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs), significantly change in ionic liquids. In this work, our purpose was to study DNA-Ag NCs in a buffer containing the hydrated ionic liquid of choline dihydrogen phosphate (choline dhp) to improve fluorescence for application in DNA detection. Due to the stabilisation of an i-motif structure by the choline cation, a unique fluorescence emission—that was not seen in an aqueous buffer—was observed in choline dhp and remained stable for more than 30 days. A DNA-Ag NCs probe was designed to have greater fluorescence intensity in choline dhp in the presence of a target DNA. A turn-on sensing platform in choline dhp was built for the detection of the BRCA1 gene, which is related to familial breast and ovarian cancers. This platform showed better sensitivity and selectivity in distinguishing a target sequence from a mutant sequence in choline dhp than in the aqueous buffer. Our study provides new evidence regarding the effects of structure on properties of fluorescent DNA-Ag NCs and expands the applications of fluorescent DNA-Ag NCs in an ionic liquid because of improved sensitivity and selectivity.

Triplex molecular beacons for sensitive recognition of melamine based on abasic-site-containing DNA and fluorescent silver nanoclusters

2015 ◽  
Vol 51 (37) ◽  
pp. 7958-7961 ◽  
Author(s):  
Ya Wang ◽  
Qianqian Sun ◽  
Linling Zhu ◽  
Junying Zhang ◽  
Fengyang Wang ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Molecular Beacons ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Abasic Site ◽  
Turn On ◽  
Ag Ncs

A label-free and turn-on strategy based on a triplex molecular beacon for fluorescence melamine recognition with the signaling of Ag NCs was developed.

scholarly journals An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

RSC Advances ◽  
2020 ◽  
Vol 10 (58) ◽  
pp. 35374-35380
Author(s):  
Baozhu Zhang ◽  
Chunying Wei
Keyword(s):  
Target Recognition ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Ag Nanoclusters ◽  
Ag Ncs

A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs).

Silver nanocluster-lightened hybridization chain reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57502-57506 ◽  
Author(s):  
Lin Liu ◽  
Qing Li ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Nucleic Acid ◽  
Silver Nanoclusters ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Silver Nanocluster ◽  
Turn On ◽  
Nucleic Acid Assays

A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

A label-free turn-on fluorescence probe for rapidly distinguishing cysteine over glutathione in water solution

2016 ◽  
Vol 500 ◽  
pp. 1-5 ◽  
Author(s):  
Liqiang Yan ◽  
Zhineng Kong ◽  
Wei Shen ◽  
Wenqi Du ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Water Solution ◽  
Label Free ◽  
Turn On
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