scholarly journals Mechanically strong interpenetrating network hydrogels for differential cellular adhesion

RSC Advances ◽  
2017 ◽  
Vol 7 (29) ◽  
pp. 18046-18053 ◽  
Author(s):  
Chong Shen ◽  
Yuyan Li ◽  
Huadi Wang ◽  
Qin Meng
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cellular Adhesion ◽  
Interpenetrating Network ◽  
Tissue Engineering Scaffolds

Hydrogels as “soft-and-wet” materials have been widely used as tissue engineering scaffolds due to their similarity to natural extracellular matrix.

scholarly journals Biomimetic hydrogels with spatial- and temporal-controlled chemical cues for tissue engineering

2020 ◽  
Vol 8 (12) ◽  
pp. 3248-3269 ◽  
Author(s):  
Weilue He ◽  
Max Reaume ◽  
Maureen Hennenfent ◽  
Bruce P. Lee ◽  
Rupak Rajachar
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Chemical Cues ◽  
Tissue Engineering Scaffolds ◽  
Spatiotemporal Characteristics

Biomimetic hydrogels work as tissue engineering scaffolds by recapitulating chemical cues and mimicking spatiotemporal characteristics of the native extracellular matrix.

scholarly journals Polymeric Scaffolds in Tissue Engineering Application: A Review

2011 ◽  
Vol 2011 ◽  
pp. 1-19 ◽  
Author(s):  
Brahatheeswaran Dhandayuthapani ◽  
Yasuhiko Yoshida ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Application ◽  
Biologically Active ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Tissue Engineering Scaffolds ◽  
Matrix Deposition ◽  
Bioactive Agents ◽  
Regenerate Tissue

Current strategies of regenerative medicine are focused on the restoration of pathologically altered tissue architectures by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable interest has been given to biologically active scaffolds which are based on similar analogs of the extracellular matrix that have induced synthesis of tissues and organs. To restore function or regenerate tissue, a scaffold is necessary that will act as a temporary matrix for cell proliferation and extracellular matrix deposition, with subsequent ingrowth until the tissues are totally restored or regenerated. Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA. Various technologies come together to construct porous scaffolds to regenerate the tissues/organs and also for controlled and targeted release of bioactive agents in tissue engineering applications. In this paper, an overview of the different types of scaffolds with their material properties is discussed. The fabrication technologies for tissue engineering scaffolds, including the basic and conventional techniques to the more recent ones, are tabulated.

Osteoblast Adhesion on Tissue Engineering Scaffolds Made by Bio-Manufacturing Techniques

2005 ◽  
Author(s):  
T. Dutta Roy ◽  
J. J. Stone ◽  
W. Sun ◽  
E. H. Cho ◽  
S. J. Lockett ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Focal Adhesion ◽  
Cellular Response ◽  
Focal Adhesions ◽  
Cellular Adhesion ◽  
Tissue Engineering Scaffolds ◽  
3D Scaffolds ◽  
Imaging Results ◽  
Proliferation And Differentiation ◽  
Manufacturing Techniques

Scientific exploration into understanding and developing relationships between three-dimensional (3D) scaffolds prepared by rapid prototyping (RP) and cellular response has focused primarily on end results targeting osteoblast proliferation and differentiation. Here at the National Institute of Standards and Technology (NIST), we take a systems approach to developing relationships between material properties and quantitative biological responses. This study in particular focuses on the screening of parameters controlled by RP techniques and their ability to trigger signalling events leading to cell adhesion. This pioneering research in our group also characterizes the in vitro cell-material interactions of 2D films and 3D scaffolds. From there, one can postulate on contributory factors leading to cell migration, proliferation, and differentiation. In summary, we believe that the quantitative information from this fundamental investigation will enhance our knowledge of the interactions between cells and 3D material interfaces with respect to formation of focal adhesions. This work consists of two sections — the application of imaging techniques for 3D characterization of properties and culturing of osteoblasts for size and shape determination. This includes quantifying the number of focal adhesion sites. We are using 3D RP polycaprolactone (PCL) scaffolds as this surrogate model in which to compare 2D to 3D material performance and cell interactions. Using RP bio-manufacturing techniques to fabricate tissue engineering scaffolds allows for control of pore size, strut size, and layer thickness, therefore providing adjustable parameters to study which can potentially influence, or even dynamically modulate, cellular adhesion. Imaging results after culturing for 24 h showed differences in cell morphology and spreading relative to the different structures. The focal adhesion response also varied, indicating an apparent loss of organization in 3D scaffolds compared to 2D surfaces. See Results and Discussion for details.

scholarly journals Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix

2007 ◽  
pp. S51-S60
Author(s):  
L Koláčná ◽  
J Bakešová ◽  
F Varga ◽  
E Košťáková ◽  
D Lukáš ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Structural Integrity ◽  
Dominant Role ◽  
Three Dimensional ◽  
Biomechanical Properties ◽  
Biophysical Properties ◽  
Tissue Engineering Scaffolds ◽  
Artificial Tissue ◽  
Cell Growth And Proliferation

ECM is composed of different collagenous and non-collagenous proteins. Collagen nanofibers play a dominant role in maintaining the biological and structural integrity of various tissues and organs, including bone, skin, tendon, blood vessels, and cartilage. Artificial collagen nanofibers are increasingly significant in numerous tissue engineering applications and seem to be ideal scaffolds for cell growth and proliferation. The modern tissue engineering task is to develop three-dimensional scaffolds of appropriate biological and biomechanical properties, at the same time mimicking the natural extracellular matrix and promoting tissue regeneration. Furthermore, it should be biodegradable, bioresorbable and non-inflammatory, should provide sufficient nutrient supply and have appropriate viscoelasticity and strength. Attributed to collagen features mentioned above, collagen fibers represent an obvious appropriate material for tissue engineering scaffolds. The aim of this minireview is, besides encapsulation of the basic biochemical and biophysical properties of collagen, to summarize the most promising modern methods and technologies for production of collagen nanofibers and scaffolds for artificial tissue development.

scholarly journals Fabrication of Biodegradable Polyester Nanocomposites by Electrospinning for Tissue Engineering

2011 ◽  
Vol 2011 ◽  
pp. 1-18 ◽  
Author(s):  
Zhi-Cai Xing ◽  
Seung-Jin Han ◽  
Yong-Suk Shin ◽  
Inn-Kyu Kang
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Biodegradable Polymers ◽  
Functional Properties ◽  
Interfacial Adhesion ◽  
Synthetic Polymers ◽  
Biodegradable Polyester ◽  
Tissue Engineering Scaffolds ◽  
Structural And Functional Properties ◽  
Enhanced Properties

Recently, nanocomposites have emerged as an efficient strategy to upgrade the structural and functional properties of synthetic polymers. Polyesters have attracted wide attention because of their biodegradability and biocompatibility. A logic consequence has been the introduction of natural extracellular matrix (ECM) molecules, organic or inorganic nanostructures to biodegradable polymers to produce nanocomposites with enhanced properties. Consequently, the improvement of the interfacial adhesion between biodegradable polymers and natural ECM molecules or nanostructures has become the key technique in the fabrication of nanocomposites. Electrospinning has been employed extensively in the design and development of tissue engineering scaffolds to generate nanofibrous substrates of synthetic biodegradable polymers and to simulate the cellular microenvironment. In this paper, several types of biodegradable polyester nanocomposites were prepared by electrospinning, with the aim of being used as tissue engineering scaffolds. The combination of biodegradable nanofibrous polymers and natural ECM molecules or nanostructures opens new paradigms for tissue engineering applications.

Use of Soft Lithography for Multi-layer MicroMolding (MMM) of 3-D PCL Scaffolds for Tissue Engineering

2004 ◽  
Vol 820 ◽  
Author(s):  
Yang Sun ◽  
Nicholas Ferrell ◽  
Derek J. Hansford
Keyword(s):  
Tissue Engineering ◽  
Soft Lithography ◽  
Cellular Adhesion ◽  
High Porosity ◽  
Grid Pattern ◽  
Tissue Engineering Scaffolds ◽  
Cell Dimensions ◽  
Orthogonal Grid ◽  
Typical Cell ◽  
Micromolding In Capillaries

AbstractTissue engineering scaffolds with precisely controlled geometries, particularly with surface features smaller than typical cell dimensions (1-10μm), can improve cellular adhesion and functionality. In this paper, soft lithography was used to fabricate polydimethylsiloxane (PDMS) stamps of arrays of parallel 5μm wide, 5μm deep grooves separated by 45 μm ridges, and an orthogonal grid of lines with the same geometry. Several methods were compared for the fabrication of 3-D multi-layer polycaprolactone (PCL) scaffolds with precise features. First, micromolding in capillaries (MIMIC) was used to deliver the polymer into the small grooves by capillarity; however the resultant lines were discontinuous and not able to form complete lines. Second, spin coating and oxygen plasma were combined to build 3-D scaffolds with the line pattern. The resultant scaffolds had good alignment and adhesion between layers; however, the upper layer collapsed due to the poor mechanical rigidity. Finally, a new multi-layer micromolding (MMM) method was developed and successfully applied with the grid pattern to fabricate 3-D scaffolds. Scanning electron microscopy (SEM) characterization showed that the multi-layered scaffolds had high porosity and precisely controlled 3-D structures.

Use of Soft Lithography for Multi-layer MicroMolding (MMM) of 3-D PCL Scaffolds for Tissue Engineering

2004 ◽  
Vol 823 ◽  
Author(s):  
Yang Sun ◽  
Nicholas Ferrell ◽  
Derek J. Hansford
Keyword(s):  
Tissue Engineering ◽  
Soft Lithography ◽  
Cellular Adhesion ◽  
High Porosity ◽  
Grid Pattern ◽  
Tissue Engineering Scaffolds ◽  
Cell Dimensions ◽  
Orthogonal Grid ◽  
Typical Cell ◽  
Micromolding In Capillaries

AbstractTissue engineering scaffolds with precisely controlled geometries, particularly with surface features smaller than typical cell dimensions (1-10μm), can improve cellular adhesion and functionality. In this paper, soft lithography was used to fabricate polydimethylsiloxane (PDMS) stamps of arrays of parallel 5μm wide, 5μm deep grooves separated by 45 μm ridges, and an orthogonal grid of lines with the same geometry. Several methods were compared for the fabrication of 3-D multi-layer polycaprolactone (PCL) scaffolds with precise features. First, micromolding in capillaries (MIMIC) was used to deliver the polymer into the small grooves by capillarity; however the resultant lines were discontinuous and not able to form complete lines. Second, spin coating and oxygen plasma were combined to build 3-D scaffolds with the line pattern. The resultant scaffolds had good alignment and adhesion between layers; however, the upper layer collapsed due to the poor mechanical rigidity. Finally, a new multi-layer micromolding (MMM) method was developed and successfully applied with the grid pattern to fabricate 3-D scaffolds. Scanning electron microscopy (SEM) characterization showed that the multi-layered scaffolds had high porosity and precisely controlled 3-D structures.

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