Integration of stem cell-derived exosomes with in situ hydrogel glue as a promising tissue patch for articular cartilage regeneration

Nanoscale ◽  
2017 ◽  
Vol 9 (13) ◽  
pp. 4430-4438 ◽  
Author(s):  
Xiaolin Liu ◽  
Yunlong Yang ◽  
Yan Li ◽  
Xin Niu ◽  
Bizeng Zhao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
In Situ Hydrogel ◽  
Articular Cartilage Regeneration

In vivo articular cartilage regeneration through infrapatellar adipose tissue derived stem cell in nanofiber polycaprolactone scaffold

2019 ◽  
Vol 57 ◽  
pp. 49-56 ◽  
Author(s):  
Parviz Vahedi ◽  
Seyedhosein Jarolmasjed ◽  
Hajar Shafaei ◽  
Leila Roshangar ◽  
Jafar Soleimani Rad ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Articular Cartilage Regeneration

scholarly journals Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration

2021 ◽  
Vol 2021 ◽  
pp. 1-25
Author(s):  
Shuangpeng Jiang ◽  
Guangzhao Tian ◽  
Xu Li ◽  
Zhen Yang ◽  
Fuxin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Research Progress ◽  
Therapeutic Effects ◽  
Physical And Mental Health ◽  
Paracrine Effects ◽  
Regeneration Strategies ◽  
Articular Cartilage Regeneration

Injury of articular cartilage can cause osteoarthritis and seriously affect the physical and mental health of patients. Unfortunately, current surgical treatment techniques that are commonly used in the clinic cannot regenerate articular cartilage. Regenerative medicine involving stem cells has entered a new stage and is considered the most promising way to regenerate articular cartilage. In terms of theories on the mechanism, it was thought that stem cell-mediated articular cartilage regeneration was achieved through the directional differentiation of stem cells into chondrocytes. However, recent evidence has shown that the stem cell secretome plays an important role in biological processes such as the immune response, inflammation regulation, and drug delivery. At the same time, the stem cell secretome can effectively mediate the process of tissue regeneration. This new theory has attributed the therapeutic effect of stem cells to their paracrine effects. The application of stem cells is not limited to exogenous stem cell transplantation. Endogenous stem cell homing and in situ regeneration strategies have received extensive attention. The application of stem cell derivatives, such as conditioned media, extracellular vesicles, and extracellular matrix, is an extension of stem cell paracrine theory. On the other hand, stem cell pretreatment strategies have also shown promising therapeutic effects. This article will systematically review the latest developments in these areas, summarize challenges in articular cartilage regeneration strategies involving stem cells, and describe prospects for future development.

Cell-Homing Strategies for in Situ Articular Cartilage Regeneration

2020 ◽  
Author(s):  
Zhen Yang ◽  
Hao Li ◽  
Weimin Guo ◽  
Zhiguo Yuan ◽  
Liwei Fu ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Cell Homing ◽  
Articular Cartilage Regeneration

Current Intelligent Injectable Hydrogels for In Situ Articular Cartilage Regeneration

2019 ◽  
Vol 60 (2) ◽  
pp. 203-225 ◽  
Author(s):  
Youguo Liao ◽  
Qiulin He ◽  
Feifei Zhou ◽  
Jingwei Zhang ◽  
Renjie Liang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Injectable Hydrogels ◽  
Articular Cartilage Regeneration

In Situ Recruitment of Human Bone Marrow-Derived Mesenchymal Stem Cells Using Chemokines for Articular Cartilage Regeneration

2015 ◽  
Vol 24 (6) ◽  
pp. 1067-1083 ◽  
Author(s):  
Min Sung Park ◽  
Yun Hee Kim ◽  
Youngmee Jung ◽  
Soo Hyun Kim ◽  
Jong Chul Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Articular Cartilage Regeneration

scholarly journals Hierarchical macro-microporous WPU-ECM scaffolds combined with Microfracture Promote in Situ Articular Cartilage Regeneration in Rabbits

2021 ◽  
Vol 6 (7) ◽  
pp. 1932-1944
Author(s):  
Mingxue Chen ◽  
YangYang Li ◽  
Shuyun Liu ◽  
Zhaoxuan Feng ◽  
Hao Wang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Articular Cartilage Regeneration

scholarly journals Biofunctionalized Structure and Ingredient Mimicking Scaffolds Achieving Recruitment and Chondrogenesis for Staged Cartilage Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhen Yang ◽  
Hao Li ◽  
Yue Tian ◽  
Liwei Fu ◽  
Cangjian Gao ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Growth Factor ◽  
Chondrogenic Differentiation ◽  
Cartilage Regeneration ◽  
Recruitment Strategy ◽  
Prolonged Release ◽  
Cell Recruitment ◽  
Articular Cartilage Regeneration

It remains scientifically challenging to regenerate injured cartilage in orthopedics. Recently, an endogenous cell recruitment strategy based on a combination of acellular scaffolds and chemoattractants to specifically and effectively recruit host cells and promote chondrogenic differentiation has brought new hope for in situ articular cartilage regeneration. In this study, a transforming growth factor-β3 (TGF-β3)-loaded biomimetic natural scaffold based on demineralized cancellous bone (DCB) and acellular cartilage extracellular matrix (ECM) was developed and found to improve chondral repair by enhancing cell migration and chondrogenesis. The DCB/ECM scaffold has porous microstructures (pore size: 67.76 ± 8.95 μm; porosity: 71.04 ± 1.62%), allowing the prolonged release of TGF-β3 (up to 50% after 42 days in vitro) and infrapatellar fat pad adipose-derived stem cells (IPFSCs) that maintain high cell viability (>96%) and favorable cell distribution and phenotype after seeding onto the DCB/ECM scaffold. The DCB/ECM scaffold itself can also provide a sustained release system to effectively promote IPFSC migration (nearly twofold in vitro). Moreover, TGF-β3 loaded on scaffolds showed enhanced chondrogenic differentiation (such as collagen II, ACAN, and SOX9) of IPFSCs after 3 weeks of culture. After implanting the composite scaffold into the knee joints of rabbits, enhanced chondrogenic differentiation was discovered at 1, 2, and 4 weeks post-surgery, and improved repair of cartilage defects in terms of biochemical, biomechanical, radiological, and histological results was identified at 3 and 6 months post-implantation. To conclude, our study demonstrates that the growth factor (GF)-loaded scaffold can facilitate cell homing, migration, and chondrogenic differentiation and promote the reconstructive effects of in vivo cartilage formation, revealing that this staged regeneration strategy combined with endogenous cell recruitment and pro-chondrogenesis is promising for in situ articular cartilage regeneration.

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